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However, bone fragility has emerged as a side effect in some but not in all human studies. Because use of SGLT2 inhibitors in humans affects mineral metabolism, we investigated the long\u2010term effects of genetic loss of Sglt2 function on bone and mineral metabolism in mice.\n                    <jats:italic>Slc5a2<\/jats:italic>\n                    nonsense mutation in Sweet Pee (SP) mice results in total loss of Sglt2 function. We collected urine, serum, and bone samples from 15\u2010week\u2010old and 25\u2010week\u2010old wild\u2010type (WT) and SP mice fasted from food overnight. We measured parameters of renal function and mineral metabolism and we assessed bone growth, microarchitecture, and mineralization. As expected, 15\u2010week\u2010old and 25\u2010week\u2010old SP mice showed increased glucosuria, and normal kidney function compared to age\u2010matched WT mice. At 15\u2009weeks, SP mice did not show alterations in mineral metabolism parameters. At 25\u2009weeks, SP mice showed reduced fasting 24\u2010hour urinary calcium excretion and increased fractional excretion of phosphate, but normal serum calcium and phosphate, parathyroid hormone (PTH), vitamin D (1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D), and fibroblast growth factor (FGF23) levels. At 25\u2009weeks, but not at 15\u2009weeks, SP mice showed reduced body weight compared to WT. This was associated with reduced femur length at 25\u2009weeks, suggesting impaired skeletal growth. SP mice did not show trabecular or cortical bone microarchitectural modifications but showed reduced cortical bone mineral density compared to WT mice at 25\u2009weeks. These results suggest that loss of Sglt2 function in mice in the absence of T2DM does not alter regulatory hormones FGF23, PTH, and 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D, but may contribute to bone fragility over the long term. Future studies are required to determine how loss of Sglt2 function impacts bone fragility in T2DM. \u00a9 2021 The Authors.\n                    <jats:italic>JBMR Plus<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.\n                  <\/jats:p>","DOI":"10.1002\/jbm4.10526","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T07:10:18Z","timestamp":1624000218000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Long\u2010Term Effects of\n                    <scp>Sglt2<\/scp>\n                    Deletion on Bone and Mineral Metabolism in Mice"],"prefix":"10.1093","volume":"5","author":[{"given":"Claire","family":"Gerber","sequence":"first","affiliation":[{"name":"Division of Nephrology and Hypertension, Feinberg School of Medicine Northwestern University  Chicago IL USA"},{"name":"Center for 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