{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:59:20Z","timestamp":1789675160709,"version":"4.0.1"},"reference-count":79,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T00:00:00Z","timestamp":1638489600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JBMR Plus"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The assay of vitamin D that began in the 1970s with the quantification of one or two metabolites, 25\u2010OH\u2010D or 1,25\u2010(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D, continues to evolve with the emergence of liquid chromatography tandem mass spectrometry (LC\u2010MS\/MS) as the technique of choice. This highly accurate, specific, and sensitive technique has been adopted by many fields of endocrinology for the measurement of multiple other components of the metabolome, and its advantage is that it not only makes it feasible to assay 25\u2010OH\u2010D or 1,25\u2010(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D but also other circulating vitamin D metabolites in the vitamin D metabolome. In the process, this broadens the spectrum of vitamin D metabolites, which the clinician can use to evaluate the many complex genetic and acquired diseases of calcium and phosphate homeostasis involving vitamin D. Several examples are provided in this review that additional metabolites (eg, 24,25\u2010(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    , 25\u2010OH\u2010D\n                    <jats:sub>3<\/jats:sub>\n                    \u201026,23\u2010lactone, and 1,24,25\u2010(OH)\n                    <jats:sub>3<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    ) or their ratios with the main forms offer valuable additional diagnostic information. This approach illustrates that biomarkers of disease can also include metabolites devoid of biological activity. Herein, a case is presented that the decision to switch to a LC\u2010MS\/MS technology permits the measurement of a larger number of vitamin D metabolites simultaneously and does not need to lead to a dramatic increase in cost or complexity because the technique uses a highly versatile tandem mass spectrometer with plenty of reserve analytical capacity. Physicians are encouraged to consider adding this rapidly evolving technique aimed at evaluating the wider vitamin D metabolome toward streamlining their approach to calcium\u2010 and phosphate\u2010related disease states. \u00a9 2021 The Authors.\n                    <jats:italic>JBMR Plus<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.\n                  <\/jats:p>","DOI":"10.1002\/jbm4.10581","type":"journal-article","created":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T05:20:36Z","timestamp":1636694436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Diagnostic Aspects of Vitamin D: Clinical Utility of Vitamin D Metabolite Profiling"],"prefix":"10.1093","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7402-3473","authenticated-orcid":false,"given":"Glenville","family":"Jones","sequence":"first","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University  Kingston Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0163-2205","authenticated-orcid":false,"given":"Martin","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences Queen's University  Kingston Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.04.070184.002425"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)93278-3"},{"key":"e_1_2_9_4_1","first-page":"200","article-title":"Structure\u2010function relationships in the vitamin D endocrine system","volume":"16","author":"Bouillon R","year":"1995","journal-title":"Endocr Rev."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00273-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-381978-9.10015-0"},{"key":"e_1_2_9_7_1","unstructured":"Vitamin D External Quality Assessment Scheme (DEQAS) [Internet]. Charing Cross Hospital London. Available at:http:\/\/www.deqas.org."},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra070553"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4306"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-33-3-554"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085035"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/46.10.1657"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2007.tb00348.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/24.2.287"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9026862"},{"key":"e_1_2_9_16_1","unstructured":"CarterG Jones J Walker Eet al. DEQAS annual reports 2017 [Internet]. Charing Cross Hospital London. Available at:http:\/\/www.deqas.org."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2010.03.010"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.121483"},{"key":"e_1_2_9_19_1","first-page":"32","article-title":"Vitamin D standardization program (VDSP). Vitamin D status as an international issue: national surveys and the problem of standardization","volume":"243","author":"Sempos CT","year":"2012","journal-title":"Scand J Clin Lab Invest Suppl."},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.07.021"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.17-0182"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1820055"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90153-K"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-4388"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.806901"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3642"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2012.185827"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3135"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008760"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.05.018"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI98093"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.11.003"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2620173"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00430a051"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2018.12.001"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1103864"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2011.05.003"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.1.196"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-2704"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-2175"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b01861"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.17-0183"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.234955"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)90976-8"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.11.6411"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01680.x"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(80)90404-X"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(87)90078-X"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/88.2.582S"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43408-X"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(82)90181-9"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650050609"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900259"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1050391"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1982.tb00725.x"},{"key":"e_1_2_9_56_1","unstructured":"BurtLA KaufmannM JonesG BillingtonEO BoydSK&HanleyDAIs bone loss in the Calgary vitamin D study mediated by 1 25\u2010(OH)2D3 or its catabolite 1 24 25\u2010(OH)3D3? Measurements of the vitamin D metabolome. Abstract for oral presentation presented at: American Society for Bone and Mineral Research Annual Meeting; September 30\u2013October 3 2021; San Diego CA."},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.105500"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2014.09.014"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0620427"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.17-0087"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-63-4-954"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-0710"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(80)90449-X"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1308"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00826a022"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106168"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402490101"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112636"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-00295"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1502602"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2015.07.023"},{"key":"e_1_2_9_72_1","unstructured":"HeijboerAAbstract: economics of vitamin D testing: comparison of LC\u2010MS\/MS and antibody\u2010based methods. Presented at: Third International Conference on Controversies in Vitamin D; Giustina A Bilezikian JP organizers. 2020; Gubbio Italy."},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.04.020"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2018.06.031"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa238"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090818"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4426"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2018.03.026"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-071812-161203"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-4387"}],"container-title":["JBMR Plus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10581","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm4.10581","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10581","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:56:41Z","timestamp":1710410201000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmrplus\/article\/7479518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":79,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jbm4.10581"],"URL":"https:\/\/doi.org\/10.1002\/jbm4.10581","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JBM4.10581\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10581\/v1\/review1","asserted-by":"object"}]},"ISSN":["2473-4039","2473-4039"],"issn-type":[{"value":"2473-4039","type":"print"},{"value":"2473-4039","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"2021-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10581"}}