{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T04:08:15Z","timestamp":1792382895505,"version":"4.4.0"},"reference-count":90,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004954","name":"Augustinus Fonden","doi-asserted-by":"publisher","award":["19\u20102370"],"award-info":[{"award-number":["19\u20102370"]}],"id":[{"id":"10.13039\/501100004954","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007405","name":"Hjerteforeningen","doi-asserted-by":"publisher","award":["19\u2010R132\u2010A9209\u201022141"],"award-info":[{"award-number":["19\u2010R132\u2010A9209\u201022141"]}],"id":[{"id":"10.13039\/100007405","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007405","name":"Hjerteforeningen","doi-asserted-by":"publisher","award":["16\u2010R107\u2010A6720\u201022012"],"award-info":[{"award-number":["16\u2010R107\u2010A6720\u201022012"]}],"id":[{"id":"10.13039\/100007405","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JBMR Plus"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vascular calcification and bone disorder progress simultaneously in chronic kidney disease (CKD). Still, how the complex pathological mechanisms are linked is only sparsely understood. Up to now, the focus has been on the disturbed bone metabolism in developing vascular calcification. However, our group has recently demonstrated that vascular calcification has negative effects on bone formation and mineralization as shown in the bone of normal recipient rats transplanted with the calcified aorta from CKD rats. In the present in vitro study, the hypothesis of a direct crosstalk between the vasculature and bone was examined. Calcified aortas from 5\/6 nephrectomized rats and normal aortas from control rats were excised and incubated ex vivo. The calcified aorta secreted large amounts of sclerostin, dickkopf\u20101 (Dkk1), and activin A. Both normal and calcified aortas secreted frizzle\u2010related protein 4 (SFRP4). Aorta rings were co\u2010incubated with the osteoblast\u2010like cell line UMR\u2010106. The calcified aorta strongly inhibited calcium crystal formation in UMR\u2010106 cells, together with a significant upregulation of the mineralization inhibitors osteopontin and progressive ankylosis protein homolog (ANKH). The strong stimulation of osteopontin was blocked by lithium chloride, indicating involvement of Wnt\/\u03b2\u2010catenin signaling. The present in vitro study shows detrimental effects of the calcified aorta on bone cell mineralization. These findings support the hypothesis of an active role of the calcified vasculature in the systemic CKD\u2013mineral and bone disorder (CKD\u2010MBD), resulting in a pathological vascular\u2013bone tissue crosstalk. \u00a9 2022 The Authors.\n                    <jats:italic>JBMR Plus<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.\n                  <\/jats:p>","DOI":"10.1002\/jbm4.10610","type":"journal-article","created":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T11:34:52Z","timestamp":1643801692000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Calcified Vasculature in Chronic Kidney Disease Secretes Factors that Inhibit Bone Mineralization"],"prefix":"10.1093","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4012-1002","authenticated-orcid":false,"given":"Maria L.","family":"Mace","sequence":"first","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Gravesen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Herlev Hospital University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Nordholm","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Nephrology, Herlev Hospital University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soeren","family":"Egstrand","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Nephrology, Herlev Hospital University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marya","family":"Morevati","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Olgaard","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Lewin","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Nephrology, Herlev Hospital University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-6009(92)90925-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030964"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02390833"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-007-0539-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050711"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.10.1974"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3830"},{"issue":"12","key":"e_1_2_8_9_1","first-page":"S16","article-title":"Epidemiology of cardiovascular disease in chronic renal disease","volume":"9","author":"Foley RN","year":"1998","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-007-9004-6"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003920070107"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm660"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.086942"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.8.1926"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-005-0250-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000414"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4203"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfaa006"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13010026"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9182078"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2018.00196"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2017.01.023"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882-020-02192-y"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.59"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvy010"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00472.2015"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00528.2017"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.02.002"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013080818"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.06550810"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.07670712"},{"issue":"2","key":"e_1_2_8_32_1","first-page":"284","article-title":"Secreted frizzled\u2010related protein 4 predicts progression of autosomal dominant polycystic kidney disease","volume":"31","author":"Zschiedrich S","year":"2016","journal-title":"Nephrol Dial Transplant"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.16.9420"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-019-02291-2"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190820"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201936"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223001101"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0252554"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2020.06.034"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031454"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.4.2.5851"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3074"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.5.537"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1086\/318811"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11070428"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2369-14-219"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.4287"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9595-4"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs466"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfv043"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1159\/000501205"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176411"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2786"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124240"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2018.07.003"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-109001"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365513.2015.1087045"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701116200"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1272\/jnms.77.4"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2021.05.005"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8080com"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.511799"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413274200"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/35077108"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.304"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2018.00170"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.16.8455"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8552"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1630"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2372"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2011.04315.x"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-3419"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1888"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfr270"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-015-3127-9"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2372"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882-019-1452-5"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.060719"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1159\/000093277"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2021.04.001"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000611"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.07.039"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.06.016"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2019.08.001"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000095676.90936.80"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.050686"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1402396"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2019.115121"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1708322"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002406"}],"container-title":["JBMR Plus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10610","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm4.10610","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10610","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:57:00Z","timestamp":1710410220000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmrplus\/article\/7479591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":90,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/jbm4.10610"],"URL":"https:\/\/doi.org\/10.1002\/jbm4.10610","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JBM4.10610\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v2\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10610\/v1\/review1","asserted-by":"object"}]},"ISSN":["2473-4039","2473-4039"],"issn-type":[{"value":"2473-4039","type":"print"},{"value":"2473-4039","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"2021-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10610"}}