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Osteoclasts are multinucleated giant cells with a bone\u2010resorbing function that play a critical role in regulating skeletal homeostasis. Osteoclast differentiation is characterized by dramatic changes in morphology and gene expression following receptor activator of nuclear factor\u2010kappa\u2010\u0392 ligand (RANKL) stimulation. We performed single\u2010cell RNA\u2010sequencing analyses of human and murine osteoclast\u2010lineage cells (OLCs) and found that OLCs in the mitotic phase do not differentiate into mature osteoclasts. We also identified a guanosine triphosphatase (GTPase) family member, RAB38, as a highly expressed molecule in both human and murine osteoclast clusters;\n                    <jats:italic>RAB38<\/jats:italic>\n                    gene expression is associated with dynamic changes in histone modification and transcriptional regulation. Silencing\n                    <jats:italic>Rab38<\/jats:italic>\n                    expression by using short hairpin RNA (shRNA) inhibited osteoclast differentiation and maturation. In summary, we established an integrated fate map of human and murine osteoclastogenesis; this will help identify therapeutic targets in bone diseases. \u00a9 2022 The Authors.\n                    <jats:italic>JBMR Plus<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.\n                  <\/jats:p>","DOI":"10.1002\/jbm4.10631","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T13:56:04Z","timestamp":1650376564000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Interspecies Single\u2010Cell\n                    <scp>RNA<\/scp>\n                    \u2010Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets"],"prefix":"10.1093","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8912-7483","authenticated-orcid":false,"given":"Yasunori","family":"Omata","sequence":"first","affiliation":[{"name":"Department of Internal Medicine 3, Rheumatology and Immunology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg (FAU) and Universit\u00e4tsklinikum Erlangen  Erlangen Germany"},{"name":"Department of Orthopaedic Surgery, Faculty of Medicine The University of Tokyo  Tokyo Japan"},{"name":"Bone and Cartilage Regenerative Medicine, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4217-1548","authenticated-orcid":false,"given":"Hiroyuki","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Faculty of Medicine The University of Tokyo  Tokyo Japan"},{"name":"Center for Disease Biology and Integrative Medicine 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