{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:52:14Z","timestamp":1787064734762,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,2,11]],"date-time":"2023-02-11T00:00:00Z","timestamp":1676073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19K10288"],"award-info":[{"award-number":["JP19K10288"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19K19159"],"award-info":[{"award-number":["JP19K19159"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20H03888"],"award-info":[{"award-number":["JP20H03888"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K10094"],"award-info":[{"award-number":["JP20K10094"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K10178"],"award-info":[{"award-number":["JP20K10178"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K10043"],"award-info":[{"award-number":["JP21K10043"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K17089"],"award-info":[{"award-number":["JP21K17089"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K10170"],"award-info":[{"award-number":["JP22K10170"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JBMR Plus"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mesenchymal stem cell (MSC) therapy is a promising approach to curing bone diseases and disorders. In treating genetic bone disorders, MSC therapy is local or systemic transplantation of isolated and in vitro proliferated MSC rather than bone marrow transplantation. Recent evidence showed that bone marrow MSC engraftment to bone regeneration has been controversial in animal and human studies. Here, our modified bone marrow transplantation (BMT) method solved this problem. Like routine BMT, our modified method involves three steps: (i) isolation of bone marrow cells from the donor, (ii) whole\u2010body lethal irradiation to the recipient, and (iii) injection of isolated bone marrow cells into irradiated recipient mice via the tail vein. The significant modification is imported at the bone marrow isolation step. While the bone marrow cells are flushed out from the bone marrow with the medium in routine BMT, we applied the enzymes\u2019 (collagenase type 4 and dispase) integrated medium to wash out the bone marrow cells. Then, cells were incubated in enzyme integrated solution at 37\u00b0C for 10\u00a0minutes. This modification designated BMT as collagenase\u2010integrated BMT (c\u2010BMT). Notably, successful engraftment of bone marrow MSC to the new bone formation, such as osteoblasts and chondrocytes, occurs in c\u2010BMT mice, whereas routine BMT mice do not recruit bone marrow MSC. Indeed, flow cytometry data showed that c\u2010BMT includes a higher proportion of LepR\n                    <jats:sup>+<\/jats:sup>\n                    , CD51\n                    <jats:sup>+<\/jats:sup>\n                    , or RUNX2\n                    <jats:sup>+<\/jats:sup>\n                    non\u2010hematopoietic cells than BMT. These findings suggested that c\u2010BMT is a time\u2010efficient and more reliable technique that ensures the disaggregation and collection of bone marrow stem cells and engraftment of bone marrow MSC to the recipient. Hence, we proposed that c\u2010BMT might be a promising approach to curing genetic bone disorders. \u00a9 2023 The Authors.\n                    <jats:italic>JBMR Plus<\/jats:italic>\n                    published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.\n                  <\/jats:p>","DOI":"10.1002\/jbm4.10722","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T06:50:37Z","timestamp":1674456637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enzyme\u2010Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0312-2778","authenticated-orcid":false,"given":"Hotaka","family":"Kawai","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"May Wathone","family":"Oo","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyofumi","family":"Takabatake","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikue","family":"Tosa","sequence":"additional","affiliation":[{"name":"Department of Regenerative Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"},{"name":"Cartilage Biology and Regenerative Medicine Laboratory, College of Dental Medicine Columbia University Irving Medical Center  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamin","family":"Soe","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Htoo Shwe","family":"Eain","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Sanou","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeko","family":"Fushimi","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Sukegawa","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"},{"name":"Department of Oral and Maxillofacial Surgery Kagawa Prefectural Central Hospital  Takamatsu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takarada","family":"Takeshi","sequence":"additional","affiliation":[{"name":"Department of Regenerative Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Nagatsuka","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-69194-7_2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095919"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.585402"},{"issue":"13","key":"e_1_2_9_5_1","first-page":"2899","article-title":"Bone marrow cells differentiation into organ cells using stem cell therapy","volume":"20","author":"Yang YJ","year":"2016","journal-title":"Eur Rev Med Pharmacol Sci."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00268"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000469704"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.5.1227"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-287565"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132252399"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000159029.48678.93"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.24605"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.148960"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14010137"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.24370"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2485\/jhtb.29.91"},{"issue":"6","key":"e_1_2_9_17_1","first-page":"6628","article-title":"Differentiation and roles of bone marrow\u2010derived cells on the tumor microenvironment of oral squamous cell carcinoma","volume":"18","author":"Anqi C","year":"2019","journal-title":"Oncol Lett."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.06.008"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2485\/jhtb.30.347"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2016.1183667"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10783"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11885"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3530"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1439-y"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2018.01.005"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt410"},{"issue":"5","key":"e_1_2_9_27_1","first-page":"899","article-title":"Effect of TGF\u2010\u03b21\/SDF\u20101\/CXCR4 signal on BM\u2010MSCs homing in rat heart of ischemia\/perfusion injury","volume":"20","author":"Zhang SJ","year":"2016","journal-title":"Eur Rev Med Pharmacol Sci."},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3584-0_7"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199611)32:3<333::AID-JBM5>3.0.CO;2-T"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.02.134"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404626101"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-115725"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-017-0490-4"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3417"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8906945"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.798604"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-12-4452"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2020.12.029"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3432"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2632959100"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2013.36"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2012.08.003"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2010.04.008"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.51946"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc19127"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-02001-1"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0311"}],"container-title":["JBMR Plus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10722","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbm4.10722","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm4.10722","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:57:53Z","timestamp":1710410273000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmrplus\/article\/7479126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,11]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/jbm4.10722"],"URL":"https:\/\/doi.org\/10.1002\/jbm4.10722","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JBM4.10722\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JBM4.10722\/v2\/review2","asserted-by":"object"}]},"ISSN":["2473-4039","2473-4039"],"issn-type":[{"value":"2473-4039","type":"print"},{"value":"2473-4039","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,11]]},"assertion":[{"value":"2022-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10722"}}