{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:35:27Z","timestamp":1790998527000,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["SNFGrant#165510"],"award-info":[{"award-number":["SNFGrant#165510"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JBMR Plus"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteogenesis imperfecta (OI) is a genetic, collagen\u2010related bone disease that increases the incidence of bone fractures. Still, the origin of this brittle mechanical behavior remains unclear. The extracellular matrix (ECM) of OI bone exhibits a higher degree of bone mineralization (DBM), whereas compressive mechanical properties at the ECM level do not appear to be inferior to healthy bone. However, it is unknown if collagen defects alter ECM tensile properties. This study aims to quantify the tensile properties of healthy and OI bone ECM. In three transiliac biopsies (healthy <jats:italic>n<\/jats:italic>\u2009=\u20091, OI type I <jats:italic>n<\/jats:italic>\u2009=\u20091, OI type III <jats:italic>n<\/jats:italic>\u2009=\u20091), 23 microtensile specimens (gauge dimensions 10 \u00d7\u20095 \u00d7\u20092 \u03bcm<jats:sup>3<\/jats:sup>) were manufactured and loaded quasi\u2010statically under tension in vacuum condition. The resulting loading modulus and ultimate strength were extracted. Interestingly, tensile properties in OI bone ECM were not inferior compared to controls. All specimens revealed a brittle failure behavior. Fracture surfaces were graded according to their mineralized collagen fibers (MCF) orientation into axial, mixed, and transversal fracture surface types (FST). Furthermore, tissue mineral density (TMD) of the biopsy cortices was extracted from micro\u2013computed tomogra[hy (\u03bcCT) images. Both FST and TMD are significant factors to predict loading modulus and ultimate strength with an adjusted <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> of 0.556 (<jats:italic>p<\/jats:italic>\u2009=\u20092.65e\u221205) and 0.46 (<jats:italic>p<\/jats:italic>\u2009=\u20092.2e\u221204), respectively. The influence of MCF orientation and DBM on the mechanical properties of the neighboring ECM was further verified with quantitative polarized Raman spectroscopy (qPRS) and site\u2010matched nanoindentation. MCF orientation and DBM were extracted from the qPRS spectrum, and a second mechanical model was developed to predict the indentation modulus with MCF orientation and DBM (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u200967.4%, <jats:italic>p<\/jats:italic>\u2009=\u20097.73e\u221207). The tensile mechanical properties of the cortical bone ECM of two OI iliac crest biopsies are not lower than the one from a healthy and are primarily dependent on MCF orientation and DBM. \u00a9 2023 The Authors. <jats:italic>JBMR Plus<\/jats:italic> published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.<\/jats:p>","DOI":"10.1002\/jbm4.10826","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T06:57:07Z","timestamp":1697093827000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-4184","authenticated-orcid":false,"given":"Michael","family":"Indermaur","sequence":"first","affiliation":[{"name":"ARTORG Center for Biomedical Engineering University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2113-5070","authenticated-orcid":false,"given":"Daniele","family":"Casari","sequence":"additional","affiliation":[{"name":"ARTORG Center for Biomedical Engineering University of Bern  Bern Switzerland"},{"name":"Swiss Federal Laboratories for Material Science and Technology  Thun Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0915-3295","authenticated-orcid":false,"given":"Tatiana","family":"Kochetkova","sequence":"additional","affiliation":[{"name":"Swiss Federal Laboratories for Material Science and Technology  Thun Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2907-3580","authenticated-orcid":false,"given":"Bettina M.","family":"Willie","sequence":"additional","affiliation":[{"name":"Research Centre, Shriners Hospital for Children\u2010Canada, Department of Pediatric Surgery McGill University  Montreal QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8860-4068","authenticated-orcid":false,"given":"Johann","family":"Michler","sequence":"additional","affiliation":[{"name":"Swiss Federal Laboratories for Material Science and Technology  Thun Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6689-2828","authenticated-orcid":false,"given":"Jakob","family":"Schwiedrzik","sequence":"additional","affiliation":[{"name":"Swiss Federal Laboratories for Material Science and Technology  Thun 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