{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:52:10Z","timestamp":1765975930796,"version":"3.48.0"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["1424\/22"],"award-info":[{"award-number":["1424\/22"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Intervertebral disc (IVD) degeneration is a leading contributor to chronic low back pain, imposing a substantial global burden. Native IVD tissue relies on sulfated glycosaminoglycans (sGAG) to maintain hydration and mechanical function through fixed negative charges and associated osmotic pressure. With aging, sGAG depletion leads to matrix disorganization, dehydration, and pathological nerve ingrowth\u2014hallmarks of disc degeneration. Synthetic GAG analog hydrogels have recently gained attention as biomimetic candidates for disc repair, owing to their structural resemblance and fixed charge density similarity to native sGAGs. Beyond their biomechanical role, these GAG analogs are hypothesized to act as neuroinhibitory barriers. In this study, we evaluated the neuroinhibitory capability of GAG analogs at varying crosslinking densities (0.5%\u20132%), using SH\u2010SY5Y neuroblastoma cells. Grown on GAG analogs, neurite extension was markedly suppressed, with average neurite lengths &lt;\u200910\u2009\u03bcm, compared to 79.3\u2009\u00b1\u200955.8 and 157.1\u2009\u00b1\u2009103.8 \u03bcm in control cultures. Importantly, cell viability remained high (75%\u201392%), irrespective of the presence of exogenous chondroitin sulfate (CS). These findings suggest that, beyond mimicking the mechanical properties of native NP, GAG analogs can also recapitulate the neuroinhibitory roles of native sGAG, underscoring their promise as therapeutic biomaterials for IVD regeneration and the prevention of pain\u2010related nerve infiltration.<\/jats:p>","DOI":"10.1002\/jbma.70009","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T13:16:17Z","timestamp":1764854177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biomimetic Glycosaminoglycan Analog Hydrogels Inhibit Neurite Outgrowth While Supporting Neuronal Cell Viability"],"prefix":"10.1002","volume":"113","author":[{"given":"Khyal","family":"Sumaya","sequence":"first","affiliation":[{"name":"Department of Biotechnology Engineering Braude College of Engineering  Karmiel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dar","family":"Segev","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Engineering Braude College of Engineering  Karmiel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haim S.","family":"Mordechai","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering &amp; Mechatronics Ariel University  Ariel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malka","family":"Shilo","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Ariel University  Ariel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirit","family":"Sharabi","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering &amp; Mechatronics Ariel University  Ariel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarit S.","family":"Sivan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Engineering Braude College of Engineering  Karmiel Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12178-014-9253-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-014-3305-z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000146101.53784.b1"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3233\/BIR-1985-22205"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208109160234"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-013-2767-8"},{"issue":"10","key":"e_1_2_11_8_1","first-page":"1298","article-title":"The Pathogenesis of Degeneration of the Intervertebral Disc and Emerging Therapies in the Management of Back Pain","volume":"94","author":"Hughes S. 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