{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:35Z","timestamp":1786977995748,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The in\u00a0vitro expansion of mesenchymal stromal cells (MSCs) is essential to produce clinically relevant quantities of cells while preserving therapeutic potential. Currently, premature senescence of the MSCs during in\u00a0vitro expansion is a significant limitation that inflates costs and reduces the efficacy of treatments. Culture environments that maintain MSC properties during in\u00a0vitro expansion are urgently needed. In this study, we explored the use of the decellularized extracellular matrix (dECM) deposited from different sources of MSCs for induced pluripotent stem cell (iPSC\u2010MSC) expansion. Specifically, we compared dECMs derived from primary bone marrow (BMSCs), a placenta\u2010derived MSC cell line (DMSC23s), and iPSC\u2010MSCs, against conventional substrates including tissue culture plastic (TCP), collagen type I, fibronectin, and Matrigel. We demonstrated for the first time that iPSC\u2010MSCs deposit substantially greater amounts of dECM than BMSCs and DMSC23s (35\u2010 and 2\u2010fold, respectively). Additionally, the dECM produced by the iPSC\u2010MSCs demonstrated superior properties in promoting MSC proliferation and lineage\u2010specific differentiation. Furthermore, enzyme\u2010linked immunosorbent assays revealed that dECM culture could modulate the MSC secretome. These findings suggest that iPSC\u2010MSCs and their ECM are suitable for optimizing and upscaling the in\u00a0vitro expansion of iPSC\u2010MSCs, offering potential advantages in regenerative therapies.<\/jats:p>","DOI":"10.1002\/jbma.70010","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T14:51:18Z","timestamp":1764773478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decellularized Extracellular Matrix Produced by\n                    <scp>iPSC<\/scp>\n                    \u2010Derived\n                    <scp>MSCs<\/scp>\n                    Promotes\n                    <scp>iPSC<\/scp>\n                    \u2010\n                    <scp>MSC<\/scp>\n                    Proliferation and Differentiation and Regulates Secreted Factors"],"prefix":"10.1002","volume":"113","author":[{"given":"Tao","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Graeme Clark Institute, The University of Melbourne  Parkville Victoria Australia"},{"name":"Pregnancy Research Centre, Department of Maternal\u2010Fetal Medicine Royal Women's Hospital  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Graeme Clark Institute, The University of Melbourne  Parkville Victoria Australia"},{"name":"Pregnancy Research Centre, Department of Maternal\u2010Fetal Medicine Royal Women's Hospital  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Grant","sequence":"additional","affiliation":[{"name":"Pregnancy Research Centre, Department of Maternal\u2010Fetal Medicine Royal Women's Hospital  Parkville Victoria Australia"},{"name":"Department of Obstetrics and Gynaecology Royal Women's Hospital, The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kilian","family":"Kelly","sequence":"additional","affiliation":[{"name":"Cynata Therapeutics  Cremorne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Graeme Clark Institute, The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bill","family":"Kalionis","sequence":"additional","affiliation":[{"name":"Pregnancy Research Centre, Department of Maternal\u2010Fetal Medicine Royal Women's Hospital  Parkville Victoria Australia"},{"name":"Department of Obstetrics and Gynaecology Royal Women's Hospital, The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel E.","family":"Heath","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Graeme Clark Institute, The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.04.014"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2013.50"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2013.0477"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0876-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1050-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1455-y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.141945.112"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.07.003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0137-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12839"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240802217011"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4935"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.5b01566"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2018.03.003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00808"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40883-018-0080-0"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36557"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2017.07.002"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.12.017"},{"issue":"5","key":"e_1_2_9_23_1","first-page":"1760","article-title":"Transferable Matrixes Produced From Decellularized Extracellular Matrix Promote Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells and Facilitate Scale\u2010Up","volume":"4","author":"Kusuma G. D.","year":"2018","journal-title":"ACS Biomaterials Science & Engineering"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0235-6"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.10.003"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.081"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2024.08.022"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmedt.2022.834123"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.11.049"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1TB00268F"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1091157"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2022.0227"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/aa895c"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171488"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7TB01298E"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.02.002"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxq030"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2012.04.003"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/app8030412"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152809788462635"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2011.01.034"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040391"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12313"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.24.021605.090737"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0071"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.05.002"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2013.0043"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2015.09.001"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1432"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0359"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.5c00411"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2024.06.037"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-024-01086-7"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbma.70010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:43:59Z","timestamp":1765975439000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbma.70010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":53,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/jbma.70010"],"URL":"https:\/\/doi.org\/10.1002\/jbma.70010","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-07-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70010"}}