{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T06:15:11Z","timestamp":1770617711138,"version":"3.49.0"},"reference-count":62,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021FYC2400800"],"award-info":[{"award-number":["2021FYC2400800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006249","name":"Donghua University","doi-asserted-by":"publisher","award":["2232023D\u201009"],"award-info":[{"award-number":["2232023D\u201009"]}],"id":[{"id":"10.13039\/501100006249","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Volumetric muscle loss (VML) presents a significant clinical challenge, exacerbated by the chronic inflammation and oxidative stress that impair muscle regeneration. To address this, we developed an injectable hydrogel for muscle repair by enzymatically crosslinking gelatin covalently grafted with ibuprofen (Gel\u2010IBU) using microbial transglutaminase (mTG). Ibuprofen was covalently grafted to gelatin via carbodiimide chemistry to obtain the Gel\u2010IBU conjugate, which was then prepared into an injectable hydrogel by mTG crosslinking. Solubilized Gel\u2010IBU showed good injectability and underwent sol\u2013gel transition in the presence of mTG within 5\u2009min at 37\u00b0C to form a hydrogel. The resulting hydrogel exhibited good adhesiveness to muscle tissue, facilitated by liquid penetration and mTG\u2010mediated crosslinking with tissue proteins. Sustained release of IBU endowed the hydrogel with potent inflammation\u2010modulating capacity, promoting macrophage polarization toward the anti\u2010inflammatory M2 phenotype, suppressing pro\u2010inflammatory cytokines, and reducing reactive oxygen species (ROS) and nitric oxide (NO) oxidative products. In a murine VML model, the Gel\u2010IBU hydrogel effectively attenuated local inflammation and enhanced tissue regeneration, demonstrating significant promise for clinical muscle regeneration.<\/jats:p>","DOI":"10.1002\/jbma.70014","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T19:26:26Z","timestamp":1765394786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ibuprofen\u2010Grafted Gelatin Hydrogel Modulates Inflammatory Response and Promotes Muscle Regeneration"],"prefix":"10.1002","volume":"113","author":[{"given":"Jieying","family":"Li","sequence":"first","affiliation":[{"name":"Shanghai Engineering Research Center of Nano\u2010Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering Donghua University  Shanghai PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangheng","family":"Guan","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Nano\u2010Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering Donghua University  Shanghai PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinglei","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Nano\u2010Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering Donghua University  Shanghai PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-024-03541-w"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.07.038"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.12519"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.948819"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202105883"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2023.0240"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbaf059"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01145"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.07.168"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S504924"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-019-0054-8"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2017.01.005"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.03.064"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.09.039"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.3c01784"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202304087"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compositesb.2022.110311"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202310483"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.06.022"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202002152"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45764-4"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.4c00882"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.4c00410"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000005704"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.120909"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn507488s"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c04376"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b17543"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b04956"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbaf089"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c02542"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112216"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-007-0013-x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6782"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0230-3"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030835"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/805172"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202506476"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-018-0027-8"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0455-7"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.650289"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2018.06.058"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.37758"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.10.009"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/abfff2"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.10.067"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42825-020-00043-y"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/15502783.2024.2302046"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2025.123268"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2009.08.014"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.06.026"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2020.04.006"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4761427"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.12.041"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.04.006"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.12.029"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3BM60198F"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-025-01698-x"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S516533"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7906"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1185-7"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2024.122174"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbma.70014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:43:52Z","timestamp":1765975432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbma.70014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":62,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/jbma.70014"],"URL":"https:\/\/doi.org\/10.1002\/jbma.70014","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70014"}}