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In this study, \u03b2\u2010cyclodextrin\u2010encapsulated\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene (\u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene) was formulated and investigated as a promising strategy for wound management. The inclusion complex was successfully synthesized via a simple approach and thoroughly characterized, confirming enhanced physicochemical stability, encapsulation efficiency, and thermal stability. The \u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene nanoparticles exhibited a high encapsulation efficiency of 91.97%\u2009\u00b1\u20093.42%. Antimicrobial assessments demonstrated significantly higher antibacterial efficacy of \u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene compared to free\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene against both Gram\u2010positive and Gram\u2010negative bacteria. A significant 90\u2010fold decrease in the minimum inhibitory concentration (MIC) was recorded for the most susceptible bacterium,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), with values decreasing from 111.2\u2009mg\/mL for free\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene to 1.24\u2009mg\/mL for the \u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene complex. In\u00a0vivo, wound healing studies revealed faster wound closure, reduced inflammation, and improved tissue regeneration. Gene expression analysis demonstrated modulation of key markers such as IL\u20106, MMP3, BAX, VEGF, and TGF\u2010\u03b21, supporting the inclusion complex's (\u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene) role in regulating inflammation, apoptosis, and angiogenesis. Histological and immunohistochemical evaluations confirmed enhanced tissue architecture and cellular response in \u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene\u2010treated mice. These results underscore the potential of \u03b2CD\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010limonene as a stable, biocompatible, and highly effective therapy, offering a natural platform for wound care and related biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/jbma.70016","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:35:07Z","timestamp":1767382507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["\u03b2\u2010Cyclodextrin Inclusion Complex of\n                    <scp>d<\/scp>\n                    \u2010Limonene: A Promising Biocompatible Formula for Enhanced Wound Healing\u2014In Vitro and In\u00a0Vivo Studies"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8783-4515","authenticated-orcid":false,"given":"Mostafa","family":"Fytory","sequence":"first","affiliation":[{"name":"Department of Chemistry School of Sciences &amp; 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Engineering, The American University in Cairo  New Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amany A.","family":"Aboomeirah","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Sciences &amp; Engineering, The American University in Cairo  New Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihad","family":"El\u2010Qassas","sequence":"additional","affiliation":[{"name":"Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology  Giza Egypt"},{"name":"Zoology Department, Faculty of Science Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Tombelli","sequence":"additional","affiliation":[{"name":"Istituto di Fisica Applicata Nello Carrara \u2013 CNR  Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3781-9709","authenticated-orcid":false,"given":"Osama M.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Physiology Division, Zoology Department, Faculty of Science Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagwa","family":"El\u2010Badri","sequence":"additional","affiliation":[{"name":"Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2047-4222","authenticated-orcid":false,"given":"Hassan Mohamed El\u2010Said","family":"Azzazy","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Sciences &amp; 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