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Woese Institute for Genomic Biology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023610","name":"Cancer Center at Illinois","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023610","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Glioblastoma is the most common primary malignant brain tumor with a 5\u2010year survival rate &lt;\u20095%. The standard of care involves surgical resection followed by treatment with the alkylating agent temozolomide (TMZ). GBM cells that evade surgery eventually become resistant to TMZ and lead to recurrence of tumors in patients. With only four drugs currently FDA\u2010approved for GBM treatment, there is a need for a clinically relevant model capable of accelerating the identification of new therapies. Microgels are microscale (~10\u20131000\u2009\u03bcm) hydrogel particles that can be used to encapsulate cells in a tailorable 3D matrix. Microdroplets offer short diffusion lengths relative to conventional hydrogel constructs (&gt;\u20091\u2009mm) to limit spatial distributions of hypoxia and potentially screen therapeutics in a controlled and physiologically relevant environment. Here, we establish a method to encapsulate GBM cells in gelatin and polyethylene glycol (PEG) microgels. We show that microgel composition can affect cell morphology and further, that collections of GBM\u2010laden hydrogels can be used to quantify the effect of single versus metronomic doses of TMZ. GBM metabolic activity is maintained in microgel culture and GBM cells display drug response kinetics similar to previously established literature using macro\u2010scale hydrogel constructs. Finally, we show microgels can be integrated with a liquid handler to enable high\u2010throughput screening using cell\u2010laden microgels.<\/jats:p>","DOI":"10.1002\/jbma.70043","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T04:51:34Z","timestamp":1770612694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hydrogel Microdroplet Based Glioblastoma Drug Screening Platform"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0529-3047","authenticated-orcid":false,"given":"Brittany A.","family":"Payan","sequence":"first","affiliation":[{"name":"Department of Bioengineering University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika Carrillo Diaz","family":"De Leon","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tejasvi","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar B.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department Chemical and Biomolecular Engineering University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishnu V.","family":"Krishnamurthy","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences High\u2010Throughput Screening Facility University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Mora\u2010Boza","sequence":"additional","affiliation":[{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta Georgia USA"},{"name":"George W. Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta Georgia USA"},{"name":"C\u00daRAM Research Ireland Centre for Medical Devices University of Galway  Galway Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6602-2518","authenticated-orcid":false,"given":"Andr\u00e9s J.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology  Atlanta Georgia USA"},{"name":"George W. Woodruff School of Mechanical Engineering Georgia Institute of Technology  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan A. C.","family":"Harley","sequence":"additional","affiliation":[{"name":"Department of Bioengineering University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Department Chemical and Biomolecular Engineering University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Cancer Center at Illinois University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noad149"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1188\/16.CJON.S1.2-8"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043330"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00840"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.105780"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14133161"},{"issue":"3","key":"e_1_2_11_8_1","article-title":"Current FDA\u2010Approved Therapies for High\u2010Grade Malignant Gliomas","volume":"9","author":"Fisher J. 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