{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T19:22:15Z","timestamp":1788376935874,"version":"build-2803163510"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T00:00:00Z","timestamp":1361318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Osteoclast (OCL) precursors from many Paget's disease (PD) patients express measles virus nucleocapsid protein (MVNP) and are hypersensitive to 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3; also know as calcitriol). The increased 1,25-(OH)2D3 sensitivity is mediated by transcription initiation factor TFIID subunit 12 (TAF12), a coactivator of the vitamin D receptor (VDR), which is present at much higher levels in MVNP-expressing OCL precursors than normals. These results suggest that TAF12 plays an important role in the abnormal OCL activity in PD. However, the molecular mechanisms underlying both 1,25-(OH)2D3's effects on OCL formation and the contribution of TAF12 to these effects in both normals and PD patients are unclear. Inhibition of TAF12 with a specific TAF12 antisense construct decreased OCL formation and OCL precursors' sensitivity to 1,25-(OH)2D3 in PD patient bone marrow samples. Further, OCL precursors from transgenic mice in which TAF12 expression was targeted to the OCL lineage (tartrate-resistant acid phosphatase [TRAP]-TAF12 mice), formed OCLs at very low levels of 1,25-(OH)2D3, although the OCLs failed to exhibit other hallmarks of PD OCLs, including receptor activator of NF-\u03baB ligand (RANKL) hypersensitivity and hypermultinucleation. Chromatin immunoprecipitation (ChIP) analysis of OCL precursors using an anti-TAF12 antibody demonstrated that TAF12 binds the 24-hydroxylase (CYP24A1) promoter, which contains two functional vitamin D response elements (VDREs), in the presence of 1,25-(OH)2D3. Because TAF12 directly interacts with the cyclic adenosine monophosphate\u2013dependent activating transcription factor 7 (ATF7) and potentiates ATF7-induced transcriptional activation of ATF7-driven genes in other cell types, we determined whether TAF12 is a functional partner of ATF7 in OCL precursors. Immunoprecipitation of lysates from either wild-type (WT) or MVNP-expressing OCL with an anti-TAF12 antibody, followed by blotting with an anti-ATF7 antibody, or vice versa, showed that TAF12 and ATF7 physically interact in OCLs. Knockdown of ATF7 in MVNP-expressing cells decreased cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1) induction by 1,25-(OH)2D3, as well as TAF12 binding to the CYP24A1 promoter. These results show that ATF7 interacts with TAF12 and contributes to the hypersensitivity of OCL precursors to 1,25-(OH)2D3 in PD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/jbmr.1884","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T21:25:07Z","timestamp":1361395507000},"page":"1489-1500","source":"Crossref","is-referenced-by-count":15,"title":["Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease"],"prefix":"10.1093","volume":"28","author":[{"given":"Jumpei","family":"Teramachi","sequence":"first","affiliation":[{"name":"Department of Medicine, Hematology OncologyIndiana UniversityIndianapolis, INUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Hiruma","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyUniversity of Pittsburgh and the Center for Bone Biology at University of Pittsburgh Medical CenterPittsburgh, PAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichi","family":"Ishizuka","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyUniversity of Pittsburgh and the Center for Bone Biology at University of Pittsburgh Medical CenterPittsburgh, PAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisako","family":"Ishizuka","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyUniversity of Pittsburgh and the Center for Bone Biology at University of Pittsburgh Medical CenterPittsburgh, PAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques P","family":"Brown","sequence":"additional","affiliation":[{"name":"Laval University, CHUQ-CHUL Research CentreQuebec, QCCanada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u00ebtitia","family":"Michou","sequence":"additional","affiliation":[{"name":"Laval University, CHUQ-CHUL Research CentreQuebec, QCCanada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiling","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyUniversity of Pittsburgh and the Center for Bone Biology at University of Pittsburgh Medical CenterPittsburgh, PAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah L","family":"Galson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyUniversity of Pittsburgh and the Center for Bone Biology at University of Pittsburgh Medical CenterPittsburgh, PAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A","family":"Subler","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular GeneticsVirginia Commonwealth UniversityRichmond, VAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of PathologyCollege of Physician and Surgeons, Columbia UniversityNew York, NYUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W","family":"Dempster","sequence":"additional","affiliation":[{"name":"Department of PathologyCollege of Physician and Surgeons, Columbia UniversityNew York, NYUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolene J","family":"Windle","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular GeneticsVirginia Commonwealth UniversityRichmond, VAUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G David","family":"Roodman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyIndiana UniversityIndianapolis, INUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyoshi","family":"Kurihara","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology OncologyIndiana UniversityIndianapolis, INUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,2,20]]},"reference":[{"key":"2024020318383564800_jbmr1884-bib-0001","volume-title":"Pathophysiology and treatment of Paget's disease of bone","author":"Kanis","year":"1998","edition":"2nd ed"},{"key":"2024020318383564800_jbmr1884-bib-0002","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1097\/00003086-198704000-00012","article-title":"Dynamic radiologic patterns of Paget's disease of bone","volume":"217","author":"Maldague","year":"1987","journal-title":"Clin Orthop Relat Res."},{"key":"2024020318383564800_jbmr1884-bib-0003","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1172\/JCI114565","article-title":"Atypical multinucleated 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