{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:17:09Z","timestamp":1791303429933,"version":"4.1.0"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,9,6]],"date-time":"2014-09-06T00:00:00Z","timestamp":1409961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81171705"],"award-info":[{"award-number":["81171705"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81190133"],"award-info":[{"award-number":["81190133"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["XDA01030404"],"award-info":[{"award-number":["XDA01030404"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"crossref","award":["12411951100"],"award-info":[{"award-number":["12411951100"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"crossref","award":["12410708600"],"award-info":[{"award-number":["12410708600"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>\u2003<\/jats:title>\n                    <jats:p>Emerging evidence indicates that microRNAs (miRNAs) play essential roles in regulating osteoblastogenesis and bone formation. However, the role of miRNA in osteoblast mechanotransduction remains to be defined. In this study, we aimed to investigate whether miRNAs regulate mechanical stimulation\u2013triggered osteoblast differentiation and bone formation through modulation of Runx2, the master transcription factor for osteogenesis. We first investigated the role of mechanical loading both in a mouse model and in an osteoblast culture system and the outcomes clearly demonstrated that mechanical stimuli can regulate osteogenesis and bone formation both in vivo and in vitro. Using bioinformatic analyses and subsequent confirmation by quantitative real-time PCR (qRT-PCR), we found that multiple miRNAs that potentially target Runx2 were responding to in vitro mechanical stimulation, among which miR-103a was fully characterized. miR-103a and its host gene PANK3 were both downregulated during cyclic mechanical stretch (CMS)-induced osteoblast differentiation, whereas Runx2 protein expression was upregulated. Overexpression of miR-103a significantly decreased and inhibition of miR-103a increased Runx2 protein level, suggesting that miR-103a acts as an endogenous attenuator of Runx2 in osteoblasts. Mutation of putative miR-103a binding sites in Runx2 mRNA abolishes miR-103a\u2013mediated repression of the Runx2 3\u2032-untranslated region (3\u2032UTR) luciferase reporter activity, suggesting that miR-103a binds to Runx2 3\u2032UTR. Osteoblast marker gene profiling and osteogenic phenotype assays demonstrated that miR-103a negatively correlates with CMS-induced osteogenesis. Further, the perturbation of miR-103a also has a significant effect on osteoblast activity and matrix mineralization. More importantly, we found an inhibitory role of miR-103a in regulating bone formation in hindlimb unloading mice, and pretreatment with antagomir-103a partly rescued the osteoporosis caused by mechanical unloading. Taken together, our data suggest that miR-103a is the first identified mechanosensitive miRNA that regulates osteoblast differentiation by directly targeting Runx2, and therapeutic inhibition of miR-103a may be an efficient anabolic strategy for skeletal disorders caused by pathological mechanical loading. \u00a9 2014 American Society for Bone and Mineral Research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbmr.2352","type":"journal-article","created":{"date-parts":[[2014,9,6]],"date-time":"2014-09-06T04:54:54Z","timestamp":1409979294000},"page":"330-345","source":"Crossref","is-referenced-by-count":148,"title":["microRNA-103a Functions as a Mechanosensitive microRNA to Inhibit Bone Formation Through Targeting Runx2"],"prefix":"10.1093","volume":"30","author":[{"given":"Bin","family":"Zuo","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JunFeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)Chinese Academy of Sciences (CAS) and Shanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChuanDong","family":"Wang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)Chinese Academy of Sciences (CAS) and Shanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoYing","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)Chinese Academy of Sciences (CAS) and Shanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GuiQuan","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)Chinese Academy of Sciences (CAS) and Shanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pathology and Laboratory MedicineThe University of Western Australia PerthAustralia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoLing","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS)Chinese Academy of Sciences (CAS) and Shanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoDong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Xinhua HospitalShanghai JiaoTong University School of Medicine (SJTUSM)ShanghaiChina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,9,6]]},"reference":[{"key":"2024020319195514900_jbmr2352-bib-0001","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nature01660","article-title":"Control of osteoblast function and regulation of bone mass","volume":"423","author":"Harada","year":"2003","journal-title":"Nature."},{"key":"2024020319195514900_jbmr2352-bib-0002","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/nm1593","article-title":"Skeletal remodeling in health and disease","volume":"13","author":"Zaidi","year":"2007","journal-title":"Nat Med."},{"key":"2024020319195514900_jbmr2352-bib-0003","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1359\/jbmr.2001.16.5.918","article-title":"Effects of loading frequency on mechanically induced bone formation","volume":"16","author":"Hsieh","year":"2001","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0004","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1359\/jbmr.2002.17.3.493","article-title":"Noninvasive loading of the murine tibia: an in vivo model for the study of mechanotransduction","volume":"17","author":"Gross","year":"2002","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0005","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1359\/jbmr.2002.17.3.481","article-title":"A comparison of type I collagen, fibronectin, and vitronectin in supporting adhesion of mechanically strained osteoblasts","volume":"17","author":"Lacouture","year":"2002","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0006","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1359\/jbmr.2002.17.4.593","article-title":"Human osteoblasts' proliferative responses to strain and 17beta-estradiol are mediated by the estrogen receptor and the receptor for insulin-like growth factor I","volume":"17","author":"Cheng","year":"2002","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0007","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S8756-3282(02)00979-1","article-title":"Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways","volume":"32","author":"Kapur","year":"2003","journal-title":"Bone."},{"key":"2024020319195514900_jbmr2352-bib-0008","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1002\/jcb.22007","article-title":"Low magnitude and high frequency mechanical loading prevents decreased bone formation responses of 2T3 preosteoblasts","volume":"106","author":"Patel","year":"2009","journal-title":"J Cell Biochem."},{"key":"2024020319195514900_jbmr2352-bib-0009","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1359\/jbmr.2002.17.8.1452","article-title":"Activation of extracellular signal-regulated kinase is involved in mechanical strain inhibition of RANKL expression in bone stromal cells","volume":"17","author":"Rubin","year":"2002","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0010","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.molmed.2009.03.001","article-title":"Mechanotransduction in osteoblast regulation and bone disease","volume":"15","author":"Papachroni","year":"2009","journal-title":"Trends Mol Med."},{"key":"2024020319195514900_jbmr2352-bib-0011","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1007\/s00198-008-0597-z","article-title":"Integrins, insulin like growth factors, and the skeletal response to load","volume":"19","author":"Bikle","year":"2008","journal-title":"Osteoporos Int."},{"key":"2024020319195514900_jbmr2352-bib-0012","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1016\/j.febslet.2007.04.037","article-title":"Load application induces changes in the expression levels of Sox-9. FGFR-3 and VEGF in condylar chondrocytes","volume":"581","author":"Papadopoulou","year":"2007","journal-title":"FEBS Lett."},{"key":"2024020319195514900_jbmr2352-bib-0013","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1677\/joe.1.06880","article-title":"Effect of mechanical loading on insulin-like growth factor-I gene expression in rat tibia","volume":"192","author":"Reijnders","year":"2007","journal-title":"J Endocrinol."},{"key":"2024020319195514900_jbmr2352-bib-0014","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.bone.2004.11.014","article-title":"Muscle atrophy and bone loss after 90 days' bed rest and the effects of flywheel resistive exercise and pamidronate: results from the LTBR study","volume":"36","author":"Rittweger","year":"2005","journal-title":"Bone."},{"key":"2024020319195514900_jbmr2352-bib-0015","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1196\/annals.1324.007","article-title":"Bone cell survival in microgravity: evidence that modeled microgravity increases osteoblast sensitivity to apoptogens","volume":"1027","author":"Bucaro","year":"2004","journal-title":"Ann N Y Acad Sci."},{"key":"2024020319195514900_jbmr2352-bib-0016","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1359\/jbmr.091041","article-title":"Capacity of omega-3 fatty acids or eicosapentaenoic acid to counteract weightlessness-induced bone loss by inhibiting NF-kappaB activation: from cells to bed rest to astronauts","volume":"25","author":"Zwart","year":"2010","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0017","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1152\/japplphysiol.91530.2008","article-title":"Bone marrow fat accumulation after 60 days of bed rest persisted 1 year after activities were resumed along with hemopoietic stimulation: the Women International Space Simulation for Exploration study","volume":"107","author":"Trudel","year":"2009","journal-title":"J Appl Physiol."},{"key":"2024020319195514900_jbmr2352-bib-0018","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1152\/japplphysiol.01064.2012","article-title":"Bone metabolism and nutritional status during 30-day head-down-tilt bed rest","volume":"113","author":"Morgan","year":"2012","journal-title":"J Appl Physiol."},{"key":"2024020319195514900_jbmr2352-bib-0019","doi-asserted-by":"crossref","first-page":"C148","DOI":"10.1152\/ajpcell.00524.2003","article-title":"Influence of increased mechanical loading by hypergravity on the microtubule cytoskeleton and prostaglandin E2 release in primary osteoblasts","volume":"289","author":"Searby","year":"2005","journal-title":"Am J Physiol Cell Physiol."},{"key":"2024020319195514900_jbmr2352-bib-0020","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.abb.2008.02.028","article-title":"The response of bone to mechanical loading and disuse: fundamental principles and influences on osteoblast\/osteocyte homeostasis","volume":"473","author":"Skerry","year":"2008","journal-title":"Arch Biochem Biophys."},{"key":"2024020319195514900_jbmr2352-bib-0021","doi-asserted-by":"crossref","first-page":"e83662","DOI":"10.1371\/journal.pone.0083662","article-title":"Microdamage caused by fatigue loading in human cancellous bone: relationship to reductions in bone biomechanical performance","volume":"8","author":"Lambers","year":"2013","journal-title":"PLoS One."},{"key":"2024020319195514900_jbmr2352-bib-0022","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/nature02871","article-title":"The functions of animal microRNAs","volume":"431","author":"Ambros","year":"2004","journal-title":"Nature."},{"key":"2024020319195514900_jbmr2352-bib-0023","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cell.2010.09.008","article-title":"MicroRNAs and cellular phenotypy","volume":"143","author":"Kosik","year":"2010","journal-title":"Cell."},{"key":"2024020319195514900_jbmr2352-bib-0024","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs: genomics, biogenesis, mechanism, and function","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell."},{"key":"2024020319195514900_jbmr2352-bib-0025","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell."},{"key":"2024020319195514900_jbmr2352-bib-0026","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nrg1379","article-title":"MicroRNAs: small RNAs with a big role in gene regulation","volume":"5","author":"Hannon","year":"2004","journal-title":"Nat Rev Genet."},{"key":"2024020319195514900_jbmr2352-bib-0027","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1126\/science.1111444","article-title":"Ribo-gnome: the big world of small RNAs","volume":"309","author":"Zamore","year":"2005","journal-title":"Science."},{"key":"2024020319195514900_jbmr2352-bib-0028","doi-asserted-by":"crossref","first-page":"20794","DOI":"10.1073\/pnas.0909311106","article-title":"A microRNA regulatory mechanism of osteoblast differentiation","volume":"106","author":"Inose","year":"2009","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0029","doi-asserted-by":"crossref","first-page":"13906","DOI":"10.1073\/pnas.0804438105","article-title":"A microRNA signature for a BMP2-induced osteoblast lineage commitment program","volume":"105","author":"Li","year":"2008","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0030","doi-asserted-by":"crossref","first-page":"19879","DOI":"10.1073\/pnas.1007698107","article-title":"A network connecting Runx2, SATB2, and the miR-23a\u223c27a\u223c 24-2 cluster regulates the osteoblast differentiation program","volume":"107","author":"Hassan","year":"2010","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0031","doi-asserted-by":"crossref","first-page":"9863","DOI":"10.1073\/pnas.1018493108","article-title":"A program of microRNAs controls osteogenic lineage progression by targeting transcription factor Runx2","volume":"108","author":"Zhang","year":"2011","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0032","doi-asserted-by":"crossref","first-page":"6139","DOI":"10.1073\/pnas.1016758108","article-title":"MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo","volume":"108","author":"Eskildsen","year":"2011","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0033","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/ng1015","article-title":"Core-binding factor beta interacts with Runx2 and is required for skeletal development","volume":"32","author":"Yoshida","year":"2002","journal-title":"Nat Genet."},{"key":"2024020319195514900_jbmr2352-bib-0034","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/ng1050","article-title":"Cbfbeta interacts with Runx2 and has a critical role in bone development","volume":"32","author":"Kundu","year":"2002","journal-title":"Nat Genet."},{"key":"2024020319195514900_jbmr2352-bib-0035","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1152\/japplphysiol.00969.2001","article-title":"Hindlimb unloading rodent model: technical aspects","volume":"92","author":"Morey-Holton","year":"2002","journal-title":"J Appl Physiol."},{"key":"2024020319195514900_jbmr2352-bib-0036","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1152\/japplphysiol.00498.2002","article-title":"Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice","volume":"94","author":"Wu","year":"2003","journal-title":"J Appl Physiol."},{"key":"2024020319195514900_jbmr2352-bib-0037","author":"Harris","year":"1997"},{"key":"2024020319195514900_jbmr2352-bib-0038","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/jbmr.5650100203","article-title":"Development and characterization of a conditionally immortalized human fetal osteoblastic cell line","volume":"10","author":"Harris","year":"1995","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0039","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/S0021-9290(98)00114-6","article-title":"Differential effect of steady versus oscillating flow on bone cells","volume":"31","author":"Jacobs","year":"1998","journal-title":"J Biomech."},{"key":"2024020319195514900_jbmr2352-bib-0040","doi-asserted-by":"crossref","first-page":"5651","DOI":"10.1016\/j.biomaterials.2006.07.027","article-title":"Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics","volume":"27","author":"Sun","year":"2006","journal-title":"Biomaterials."},{"key":"2024020319195514900_jbmr2352-bib-0041","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0021-9290(99)00210-9","article-title":"Quantifying the strain history of bone: spatial uniformity and self-similarity of low-magnitude strains","volume":"33","author":"Fritton","year":"2000","journal-title":"J Biomech."},{"key":"2024020319195514900_jbmr2352-bib-0042","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1054\/arth.2000.6626","article-title":"A stem design change to reduce peak cement strains at the tip of cemented total hip arthroplasty","volume":"15","author":"Estok","year":"2000","journal-title":"J Arthroplasty."},{"key":"2024020319195514900_jbmr2352-bib-0043","first-page":"184","article-title":"Strain amplification in the bone mechanosensory system","volume":"316","author":"Cowin","year":"1998","journal-title":"Am J Med Sci."},{"key":"2024020319195514900_jbmr2352-bib-0044","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/S0021-9290(01)00107-5","article-title":"A model for strain amplification in the actin cytoskeleton of osteocytes due to fluid drag on pericellular matrix","volume":"34","author":"You","year":"2001","journal-title":"J Biomech."},{"issue":"249","key":"2024020319195514900_jbmr2352-bib-0045","doi-asserted-by":"crossref","first-page":"RE12","DOI":"10.1126\/stke.2492004re12","article-title":"Signal transduction and mechanical stress","volume":"2004","author":"Hughes-Fulford","year":"2004","journal-title":"Sci STKE."},{"key":"2024020319195514900_jbmr2352-bib-0046","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1359\/jbmr.2003.18.1.58","article-title":"A short pulse of mechanical force induces gene expression and growth in MC3T3-E1 osteoblasts via an ERK 1\/2 pathway","volume":"18","author":"Hatton","year":"2003","journal-title":"J Bone Miner Res."},{"key":"2024020319195514900_jbmr2352-bib-0047","doi-asserted-by":"crossref","first-page":"20715","DOI":"10.1074\/jbc.M703224200","article-title":"Wnt\/beta-catenin signaling is a component of osteoblastic bone cell early responses to load-bearing and requires estrogen receptor alpha","volume":"282","author":"Armstrong","year":"2007","journal-title":"J Biol Chem."},{"key":"2024020319195514900_jbmr2352-bib-0048","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/stem.288","article-title":"MicroRNA-204 regulates Runx2 protein expression and mesenchymal progenitor cell differentiation","volume":"28","author":"Huang","year":"2010","journal-title":"Stem Cells."},{"key":"2024020319195514900_jbmr2352-bib-0049","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/nature10112","article-title":"MicroRNAs 103 and 107 regulate insulin sensitivity","volume":"474","author":"Trajkovski","year":"2011","journal-title":"Nature."},{"key":"2024020319195514900_jbmr2352-bib-0050","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.ymgme.2007.03.011","article-title":"Energizing miRNA research: a review of the role of miRNAs in lipid metabolism, with a prediction that miR-103\/107 regulates human metabolic pathways","volume":"91","author":"Wilfred","year":"2007","journal-title":"Mol Genet Metab."},{"key":"2024020319195514900_jbmr2352-bib-0051","doi-asserted-by":"crossref","first-page":"4446","DOI":"10.1093\/nar\/gks025","article-title":"Dataset integration identifies transcriptional regulation of microRNA genes by PPAR\u03b3 in differentiating mouse 3T3-L1 adipocytes","volume":"40","author":"John","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"2024020319195514900_jbmr2352-bib-0052","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/nature04303","article-title":"Silencing of microRNAs in vivo with \u2018antagomirs\u2019","volume":"438","author":"Kr\u00fctzfeldt","year":"2005","journal-title":"Nature."},{"key":"2024020319195514900_jbmr2352-bib-0053","doi-asserted-by":"crossref","first-page":"3234","DOI":"10.1073\/pnas.0914825107","article-title":"Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth","volume":"107","author":"Wang","year":"2010","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0054","doi-asserted-by":"crossref","first-page":"3240","DOI":"10.1073\/pnas.0914882107","article-title":"MicroRNA-19a mediates the suppressive effect of laminar flow on cyclin D1 expression in human umbilical vein endothelial cells","volume":"107","author":"Qin","year":"2010","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2024020319195514900_jbmr2352-bib-0055","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1186\/1471-2164-13-154","article-title":"MicroRNA modulate alveolar epithelial response to cyclic stretch","volume":"13","author":"Yehya","year":"2012","journal-title":"BMC Genomics."},{"key":"2024020319195514900_jbmr2352-bib-0056","doi-asserted-by":"crossref","first-page":"29336","DOI":"10.1074\/jbc.M110.101147","article-title":"Mechanical stretch up-regulates microRNA-26a and induces human airway smooth muscle hypertrophy by suppressing glycogen synthase kinase-3\u03b2","volume":"285","author":"Mohamed","year":"2010","journal-title":"J Biol Chem."},{"key":"2024020319195514900_jbmr2352-bib-0057","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1093\/eurjhf\/hfs143","article-title":"Mechanical stretch via transforming growth factor-\u03b21 activates microRNA208a to regulate endoglin expression in cultured rat cardiac myoblasts","volume":"15","author":"Shyu","year":"2013","journal-title":"Eur J Heart Fail."},{"key":"2024020319195514900_jbmr2352-bib-0058","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.1096\/fj.11-185132","article-title":"MiR-365: a mechanosensitive microRNA stimulates chondrocyte differentiation through targeting histone deacetylase 4","volume":"25","author":"Guan","year":"2011","journal-title":"FASEB J."},{"key":"2024020319195514900_jbmr2352-bib-0059","doi-asserted-by":"crossref","first-page":"4315","DOI":"10.1038\/sj.onc.1207676","article-title":"Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression","volume":"23","author":"Stein","year":"2004","journal-title":"Oncogene."},{"key":"2024020319195514900_jbmr2352-bib-0060","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0092-8674(00)80259-7","article-title":"Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development","volume":"89","author":"Otto","year":"1997","journal-title":"Cell."},{"key":"2024020319195514900_jbmr2352-bib-0061","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1074\/jbc.M509430200","article-title":"TNF promotes RUNX2 degradation through up-regulation of SMURF1 and SMURF2 in osteoblasts","volume":"281","author":"Kaneki","year":"2006","journal-title":"J Biol Chem."},{"key":"2024020319195514900_jbmr2352-bib-0062","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nm.3026","article-title":"miR-214 targets ATF4 to inhibit bone formation","volume":"19","author":"Wang","year":"2013","journal-title":"Nat Med."},{"key":"2024020319195514900_jbmr2352-bib-0063","doi-asserted-by":"crossref","first-page":"3666","DOI":"10.1172\/JCI39832","article-title":"A novel microRNA targeting HDAC5 regulates osteoblast differentiation in mice and contributes to primary osteoporosis in humans","volume":"119","author":"Li","year":"2009","journal-title":"J Clin Invest."},{"key":"2024020319195514900_jbmr2352-bib-0064","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1038\/ncb2211","article-title":"Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism","volume":"13","author":"Jordan","year":"2011","journal-title":"Nat Cell Biol."},{"key":"2024020319195514900_jbmr2352-bib-0065","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1038\/emboj.2011.249","article-title":"Bidirectional integrative regulation of Cav1.2 calcium channel by microRNA miR-103: role in pain","volume":"30","author":"Favereaux","year":"2011","journal-title":"EMBO J."},{"key":"2024020319195514900_jbmr2352-bib-0066","doi-asserted-by":"crossref","first-page":"e30221","DOI":"10.1371\/journal.pone.0030221","article-title":"Identification of miRNA-103 in the cellular fraction of human peripheral blood as a potential biomarker for malignant mesothelioma\u2014a pilot study","volume":"7","author":"Weber","year":"2012","journal-title":"PLoS One."},{"key":"2024020319195514900_jbmr2352-bib-0067","doi-asserted-by":"crossref","first-page":"2630","DOI":"10.1210\/en.2006-1372","article-title":"In vivo analysis of Wnt signaling in bone","volume":"148","author":"Glass","year":"2007","journal-title":"Endocrinology."},{"key":"2024020319195514900_jbmr2352-bib-0068","doi-asserted-by":"crossref","first-page":"849","DOI":"10.4065\/80.7.849","article-title":"Significant reduction in risk of falls and back pain in osteoporotic-kyphotic women through a Spinal Proprioceptive Extension Exercise Dynamic (SPEED) program","volume":"80","author":"Sinaki","year":"2005","journal-title":"Mayo Clin Proc."},{"issue":"1","key":"2024020319195514900_jbmr2352-bib-0069","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1083\/jcb.200105052","article-title":"Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures","volume":"155","author":"Liu","year":"2001","journal-title":"J Cell Biol."},{"key":"2024020319195514900_jbmr2352-bib-0070","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.abb.2008.02.030","article-title":"Transcription factors controlling osteoblastogenesis","volume":"473","author":"Marie","year":"2008","journal-title":"Arch Biochem Biophys."},{"key":"2024020319195514900_jbmr2352-bib-0071","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1001\/archinte.164.10.1084","article-title":"Benefits of 2 years of intense exercise on bone density, physical fitness, and blood lipids in early postmenopausal osteopenic women: results of the Erlangen Fitness Osteoporosis Prevention Study (EFOPS)","volume":"164","author":"Kemmler","year":"2004","journal-title":"Arch Intern Med."},{"issue":"385","key":"2024020319195514900_jbmr2352-bib-0072","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1097\/00003086-200104000-00030","article-title":"Effects of high-frequency, low-magnitude mechanical stimulus on bone healing","author":"Wolf","year":"2001","journal-title":"Clin Orthop Relat Res."},{"key":"2024020319195514900_jbmr2352-bib-0073","doi-asserted-by":"crossref","first-page":"S36","DOI":"10.1007\/s00198-004-1728-9","article-title":"Mechanics and mechano-biology of fracture healing in normal and osteoporotic bone","author":"Augat","year":"2005","journal-title":"Osteoporos Int."},{"key":"2024020319195514900_jbmr2352-bib-0074","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1302\/0301-620X.67B4.4030869","article-title":"The influence of induced micromovement upon the healing of experimental tibial fractures","volume":"67","author":"Goodship","year":"1985","journal-title":"J Bone Joint Surg Br."},{"key":"2024020319195514900_jbmr2352-bib-0075","doi-asserted-by":"crossref","first-page":"139","DOI":"10.2165\/00007256-200838020-00004","article-title":"Mechanotransduction in human bone: in vitro cellular physiology that underpins bone changes with exercise","volume":"38","author":"Scott","year":"2008","journal-title":"Sports Med."}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.2352","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/30\/2\/330\/56569149\/jbmr2352.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/30\/2\/330\/56569149\/jbmr2352.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T10:52:54Z","timestamp":1710327174000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/30\/2\/330-345\/7599348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,6]]},"references-count":75,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2014,9,6]]},"published-print":{"date-parts":[[2015,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.2352","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2015,2,1]]},"published":{"date-parts":[[2014,9,6]]}}}