{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:44:26Z","timestamp":1779306266380,"version":"3.51.4"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T00:00:00Z","timestamp":1421020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Signal peptide-CUB-EGF domain-containing protein 2 (SCUBE2) belongs to a secreted and membrane-tethered multidomain SCUBE protein family composed of three members found in vertebrates and mammals. Recent reports suggested that zebrafish scube2 could facilitate sonic hedgehog (Shh) signaling for proper development of slow muscle. However, whether SCUBE2 can regulate the signaling activity of two other hedgehog ligands (Ihh and Dhh), and the developmental relevance of the SCUBE2-induced hedgehog signaling in mammals remain poorly understood. In this study, we first showed that as compared with SCUBE1 or SCUBE3, SCUBE2 is the most potent modulator of IHH signaling in vitro. In addition, gain and loss-of-function studies demonstrated that SCUBE2 exerted an osteogenic function by enhancing Ihh-stimulated osteoblast differentiation in the mouse mesenchymal progenitor cells. Consistent with these in vitro studies and the prominent roles of Ihh in coordinating skeletogenesis, genetic ablation of Scube2 (\u2013\/\u2013) caused defective endochondral bone formation and impaired Ihh-mediated chondrocyte differentiation and proliferation as well as osteoblast differentiation of \u2013\/\u2013 bone-marrow mesenchymal stromal-cell cultures. Our data demonstrate that Scube2 plays a key regulatory role in Ihh-dependent endochondral bone formation. \u00a9 2015 American Society for Bone and Mineral Research.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/jbmr.2451","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T13:35:36Z","timestamp":1421069736000},"page":"1255-1267","source":"Crossref","is-referenced-by-count":45,"title":["Disruption of <i>Scube2<\/i> Impairs Endochondral Bone Formation"],"prefix":"10.1093","volume":"30","author":[{"given":"Yuh-Charn","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan"},{"name":"Graduate Institute of Life SciencesNational Defense Medical CenterTaipeiTaiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve R.","family":"Roffler","sequence":"additional","affiliation":[{"name":"Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Ting","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruey-Bing","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of PharmacologySchool of MedicineNational Yang-Ming UniversityTaipeiTaiwan"},{"name":"Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan"},{"name":"Graduate Institute of Life SciencesNational Defense Medical CenterTaipeiTaiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,1,12]]},"reference":[{"key":"2024020319051312100_jbmr2451-bib-0001","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1042\/BJ20090341","article-title":"Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans","volume":"422","author":"Tsai","year":"2009","journal-title":"Biochem J"},{"key":"2024020319051312100_jbmr2451-bib-0002","doi-asserted-by":"crossref","first-page":"46364","DOI":"10.1074\/jbc.M207410200","article-title":"Identification of a novel family of cell-surface proteins expressed in human vascular endothelium","volume":"277","author":"Yang","year":"2002","journal-title":"J Biol Chem"},{"key":"2024020319051312100_jbmr2451-bib-0003","doi-asserted-by":"crossref","first-page":"27039","DOI":"10.1074\/jbc.M111.244418","article-title":"Domain and functional analysis of a novel breast tumor suppressor protein","volume":"286","author":"Lin","year":"2011","journal-title":"SCUBE2. 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