{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:56:12Z","timestamp":1773424572788,"version":"3.50.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T00:00:00Z","timestamp":1707523200000},"content-version":"vor","delay-in-days":2497,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["102215\/2\/13\/2"],"award-info":[{"award-number":["102215\/2\/13\/2"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000883","name":"University of Bristol","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000883","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/I025751\/1 and BB\/I025263\/1"],"award-info":[{"award-number":["BB\/I025751\/1 and BB\/I025263\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>\u2003<\/jats:title>\n                  <jats:p>Genetic and environmental determinants of skeletal phenotypes such as bone mineral density (BMD) may converge through the epigenome, providing a tool to better understand osteoporosis pathophysiology. Because the epigenetics of BMD have been largely unexplored in humans, we performed an epigenome-wide association study (EWAS) of BMD. We undertook a large-scale BMD EWAS using the Infinium HumanMethylation450 array to measure site-specific DNA methylation in up to 5515 European-descent individuals (NDiscovery\u2009=\u20094614, NValidation\u2009=\u2009901). We associated methylation at multiple cytosine-phosphate-guanine (CpG) sites with dual-energy X-ray absorptiometry (DXA)-derived femoral neck and lumbar spine BMD. We performed sex-combined and stratified analyses, controlling for age, weight, smoking status, estimated white blood cell proportions, and random effects for relatedness and batch effects. A 5% false-discovery rate was used to identify CpGs associated with BMD. We identified one CpG site, cg23196985, significantly associated with femoral neck BMD in 3232 females (p\u2009=\u20097.9\u2009\u00d7\u200910\u221211) and 4614 females and males (p\u2009=\u20093.0\u2009\u00d7\u200910\u22128). cg23196985 was not associated with femoral neck BMD in an additional sample of 474 females (p\u2009=\u20090.64) and 901 males and females (p\u2009=\u20090.60). Lack of strong consistent association signal indicates that among the tested probes, no large-effect epigenetic changes in whole blood associated with BMD, suggesting future epigenomic studies of musculoskeletal traits measure DNA methylation in a different tissue with extended genome coverage. \u00a9 2017 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/jbmr.3148","type":"journal-article","created":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T11:32:41Z","timestamp":1491823961000},"page":"1644-1650","source":"Crossref","is-referenced-by-count":56,"title":["Epigenome-wide Association of DNA Methylation in Whole Blood With Bone Mineral Density"],"prefix":"10.1093","volume":"32","author":[{"given":"John A","family":"Morris","sequence":"first","affiliation":[{"name":"Lady Davis Institute for Medical ResearchDepartment of MedicineJewish General HospitalMcGill UniversityMontrealCanada"},{"name":"Department of Human GeneticsMcGill UniversityMontrealCanada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Chien","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Twin Research and Genetic EpidemiologyKing's College LondonLondonUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roby","family":"Joehanes","sequence":"additional","affiliation":[{"name":"Department of MedicineBeth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA"},{"name":"Institute for Aging ResearchHebrew SeniorLifeBostonMAUSA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zheng","sequence":"additional","affiliation":[{"name":"MRC Integrative Epidemiology UnitUniversity of BristolBristolUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Trajanoska","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine and EpidemiologyErasmus Medical CenterRotterdamNetherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette","family":"Soerensen","sequence":"additional","affiliation":[{"name":"Department of Clinical GeneticsOdense University HospitalOdenseDenmark"},{"name":"The Danish Twin Registry and The Danish Aging Research CenterEpidemiology, Biostatistics, and BiodemographyInstitute of Public HealthUniversity of Southern DenmarkOdenseDenmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Forgetta","sequence":"additional","affiliation":[{"name":"Lady Davis Institute for Medical ResearchDepartment of MedicineJewish General HospitalMcGill UniversityMontrealCanada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Edgar","family":"Castillo-Fernandez","sequence":"additional","affiliation":[{"name":"Department of Twin Research and Genetic EpidemiologyKing's College LondonLondonUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morten","family":"Frost","sequence":"additional","affiliation":[{"name":"Endocrine Research UnitKMEBUniversity of Southern DenmarkOdenseDenmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim D","family":"Spector","sequence":"additional","affiliation":[{"name":"Department of Twin Research and Genetic EpidemiologyKing's College LondonLondonUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaare","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry and PharmacologyOdense University HospitalOdenseDenmark"},{"name":"Department of Clinical GeneticsOdense University HospitalOdenseDenmark"},{"name":"The Danish Twin Registry and The Danish Aging Research CenterEpidemiology, Biostatistics, and BiodemographyInstitute of Public HealthUniversity of Southern DenmarkOdenseDenmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lene","family":"Christiansen","sequence":"additional","affiliation":[{"name":"The Danish Twin Registry and The Danish Aging Research CenterEpidemiology, Biostatistics, and BiodemographyInstitute of Public HealthUniversity of Southern DenmarkOdenseDenmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Rivadeneira","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine and EpidemiologyErasmus Medical CenterRotterdamNetherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan H","family":"Tobias","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research UnitUniversity of BristolBristolUK"},{"name":"MRC Integrative Epidemiology UnitUniversity of BristolBristolUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M","family":"Evans","sequence":"additional","affiliation":[{"name":"The University of Queensland Diamantina InstituteTranslational Research InstitutePrincess Alexandra HospitalBrisbaneAustralia"},{"name":"MRC Integrative Epidemiology UnitUniversity of BristolBristolUK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas P","family":"Kiel","sequence":"additional","affiliation":[{"name":"Department of MedicineBeth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA"},{"name":"Institute for Aging ResearchHebrew SeniorLifeBostonMAUSA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Hsiang","family":"Hsu","sequence":"additional","affiliation":[{"name":"Broad Institute of MIT and HarvardCambridgeMAUSA"},{"name":"Department of MedicineBeth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA"},{"name":"Institute for Aging ResearchHebrew SeniorLifeBostonMAUSA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J Brent","family":"Richards","sequence":"additional","affiliation":[{"name":"Departments of Epidemiology and Biostatistics and MedicineMcGill UniversityMontrealCanada"},{"name":"Department of Twin Research and Genetic EpidemiologyKing's College LondonLondonUK"},{"name":"Lady Davis Institute for Medical ResearchDepartment of MedicineJewish General HospitalMcGill UniversityMontrealCanada"},{"name":"Department of Human GeneticsMcGill UniversityMontrealCanada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordana T","family":"Bell","sequence":"additional","affiliation":[{"name":"Department of Twin Research and Genetic EpidemiologyKing's College LondonLondonUK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,4,10]]},"reference":[{"issue":"3","key":"2024021014200933200_jbmr3148-bib-0001","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1359\/jbmr.061113","article-title":"Incidence and economic burden of osteoporosis-related fractures in the United States, 2005-2025","volume":"22","author":"Burge","year":"2007","journal-title":"J Bone Miner Res"},{"issue":"1\u20132","key":"2024021014200933200_jbmr3148-bib-0002","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s11657-013-0136-1","article-title":"Osteoporosis in 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