{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T01:47:30Z","timestamp":1781228850352,"version":"3.54.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T00:00:00Z","timestamp":1519084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01\u2010AG039355"],"award-info":[{"award-number":["P01\u2010AG039355"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010AR051517"],"award-info":[{"award-number":["R01\u2010AR051517"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21\u2010AR062346"],"award-info":[{"award-number":["R21\u2010AR062346"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10RR027668"],"award-info":[{"award-number":["S10RR027668"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["asbmr.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>\u2003<\/jats:title>\n                    <jats:p>Type I collagen is the most abundant extracellular matrix protein in bone and other connective tissues and plays key roles in normal and pathological bone formation as well as in connective tissue disorders and fibrosis. Although much is known about the collagen biosynthetic pathway and its regulatory steps, the mechanisms by which it is assembled extracellularly are less clear. We have generated GFPtpz and mCherry-tagged collagen fusion constructs for live imaging of type I collagen assembly by replacing the \u03b12(I)-procollagen N-terminal propeptide with GFPtpz or mCherry. These novel imaging probes were stably transfected into MLO-A5 osteoblast-like cells and fibronectin-null mouse embryonic fibroblasts (FN-null-MEFs) and used for imaging type I collagen assembly dynamics and its dependence on fibronectin. Both fusion proteins co-precipitated with \u03b11(I)-collagen and remained intracellular without ascorbate but were assembled into \u03b11(I) collagen-containing extracellular fibrils in the presence of ascorbate. Immunogold-EM confirmed their ultrastuctural localization in banded collagen fibrils. Live cell imaging in stably transfected MLO-A5 cells revealed the highly dynamic nature of collagen assembly and showed that during assembly the fibril networks are continually stretched and contracted due to the underlying cell motion. We also observed that cell-generated forces can physically reshape the collagen fibrils. Using co-cultures of mCherry- and GFPtpz-collagen expressing cells, we show that multiple cells contribute collagen to form collagen fiber bundles. Immuno-EM further showed that individual collagen fibrils can receive contributions of collagen from more than one cell. Live cell imaging in FN-null-MEFs expressing GFPtpz-collagen showed that collagen assembly was both dependent upon and dynamically integrated with fibronectin assembly. These GFP-collagen fusion constructs provide a powerful tool for imaging collagen in living cells and have revealed novel and fundamental insights into the dynamic mechanisms for the extracellular assembly of collagen. \u00a9 2018 American Society for Bone and Mineral Research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbmr.3409","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T14:49:40Z","timestamp":1519224580000},"page":"1166-1182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs"],"prefix":"10.1093","volume":"33","author":[{"given":"Yongbo","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Biomedical SciencesTexas A&M University College of DentistryDallasTXUSA"},{"name":"Department of Oral and Craniofacial 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DentistryUniversity of MissouriKansas City, Kansas CityMOUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A","family":"Grillo","sequence":"additional","affiliation":[{"name":"Department of Oral and Craniofacial SciencesSchool of DentistryUniversity of MissouriKansas City, Kansas CityMOUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A","family":"Veno","sequence":"additional","affiliation":[{"name":"Department of Oral and Craniofacial SciencesSchool of DentistryUniversity of MissouriKansas City, Kansas CityMOUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Dusevich","sequence":"additional","affiliation":[{"name":"Department of Oral and Craniofacial SciencesSchool of DentistryUniversity of MissouriKansas City, Kansas CityMOUSA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte L","family":"Phillips","sequence":"additional","affiliation":[{"name":"Departments of 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