{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:40:06Z","timestamp":1790617206230,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In order to determine whether the renin-angiotensin system (RAS) has any physiologic function in bone metabolism, mice lacking the gene encoding the major angiotensin II receptor isoform, AT1a, were studied using micro CT scanning, histomorphometric, and biochemical techniques. Three-dimensional (3D) micro CT analysis of the tibial metaphysis revealed that both male and female AT1a knockout mice exhibited an increased trabecular bone volume along with increased trabecular number and connectivity. Histomorphometric analysis of the tibial metaphysis indicated that the parameters of bone formation as well as resorption were increased, which was also supported by elevated serum osteocalcin and carboxy-terminal collagen crosslink (CTX) concentrations in the AT1a-deficient mice. Osteoclastogenesis and osteoblastogenesis assays in ex vivo cultures, however, did not reveal any intrinsic alterations in the differentiation potential of AT1a-deficient cells. Quantitative RT-PCR using RNA isolated from the tibia and femur revealed that the receptor activator of NF-\u03baB ligand (RANKL)\/osteoprotegerin (OPG) ratio and the expression of stromal cell-derived factor (SDF)1\u03b1 were increased, whereas that of SOST was decreased in AT1a-deficient bone, which may account for the increased bone resorption and formation, respectively. AT1a-deficient mice also displayed a lean phenotype with reduced serum leptin levels. They maintained high bone mass with advancing age, and were protected from bone loss induced by ovariectomy. Collectively, the data suggest that RAS has a physiologic function in bone remodeling, and that signaling through AT1a negatively regulates bone turnover and bone mass. \u00a9 2011 American Society for Bone and Mineral Research<\/jats:p>","DOI":"10.1002\/jbmr.501","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T20:54:13Z","timestamp":1314910453000},"page":"2959-2966","source":"Crossref","is-referenced-by-count":48,"title":["Physiological function of the angiotensin AT1a receptor in bone remodeling"],"prefix":"10.1093","volume":"26","author":[{"given":"Keiko","family":"Kaneko","sequence":"first","affiliation":[{"name":"Department of Bone and Joint Disease, National Center for Geriatrics and Gerontology (NCGG), Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Radiology, Nagasaki University School of Medicine, Nagasaki, 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Center, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki, Japan"},{"name":"Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoji","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Bone and Joint Disease, National Center for Geriatrics and Gerontology (NCGG), Aichi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,9,1]]},"reference":[{"issue":"5","key":"2024020804160052800_bib1","doi-asserted-by":"crossref","first-page":"E686","DOI":"10.1152\/ajpendo.00552.2003","article-title":"Osteogenic regulation of vascular calcification: an early perspective","volume":"286","author":"Vattikuti","year":"2004","journal-title":"Am J Physiol Endocrinol 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