{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:35:17Z","timestamp":1761762917507,"version":"3.37.3"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1986,4,1]],"date-time":"1986-04-01T00:00:00Z","timestamp":512697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"crossref","award":["AM 19855"],"award-info":[{"award-number":["AM 19855"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1986,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mounting experimental evidence indicates that osteoblasts may be cellular intermediaries in the local activation of bone remodeling. To elucidate the role of these cells in activation, we examined the effects of prostaglandins (PGs), known resorption stimulators, on cell shape and intercellular junctional relationships in osteoblasts cultured from rat fetal calvaria. Exposure to PGE2 and PGE1, promoters of bone resorption, rapidly (within 20 min) converted the osteoblasts from a flattened to a stellate shape (shape change), and markedly increased the appearance of intercellular (gap) junctions within 10 min. Both effects were directly related to the prostaglandin concentration, as little as 1 nM being effective. PGE1, but not PGB1, PGF1\u03b1, PGD2, and PGF2\u03b1, mimicked the substantial effect of PGE2 on shape change. Shape change and gap junction formation appear to arise independently. PTH, an inducer of shape change, did not affect the number of gap junctions appreciably. Colchicine, a microtubule polymerization inhibitor, and trifluoperazine, an inhibitor of calmodulin action, blunted PGE2-mediated shape change but not the effect of PGE2 on gap junctions. Shape change and gap junction formation may be important events in local activation, shape changes in surface osteoblasts serving to expose bone surfaces which are chemotactic for osteoclasts and gap junctions propagating locally initiated activation messages.<\/jats:p>","DOI":"10.1002\/jbmr.5650010302","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T18:28:13Z","timestamp":1265221693000},"page":"243-249","source":"Crossref","is-referenced-by-count":62,"title":["Prostaglandins change cell shape and increase intercellular gap junctions in osteoblasts cultured from rat fetal calvaria"],"prefix":"10.1093","volume":"1","author":[{"given":"Victor","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Medicine, Washington University at Jewish Hospital, St. Louis, MO 63110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard","family":"Rifas","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University at Jewish Hospital, St. Louis, MO 63110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail","family":"Kohler","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University at Jewish Hospital, St. Louis, MO 63110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William A.","family":"Peck","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University at Jewish Hospital, St. Louis, MO 63110"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1986,4,1]]},"reference":[{"key":"2024012712001608300_bib1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF02409454","article-title":"Role of osteoblasts in hormonal control of bone resorption\u2014a hypothesis","volume":"33","author":"Rodan","year":"1981","journal-title":"Calcif Tissue Int"},{"key":"2024012712001608300_bib2","first-page":"244","volume-title":"Bone and Mineral Research, Annual 2","author":"Rodan","year":"1984"},{"key":"2024012712001608300_bib3","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1083\/jcb.99.6.1901","article-title":"Osteoclast formation from mononuclear phagocytes: role of bone-forming cells","volume":"99","author":"Burger","year":"1984","journal-title":"J Cell Biol"},{"key":"2024012712001608300_bib4","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0304-4165(84)90409-4","article-title":"A factor synthesized by rabbit periosteal fibroblast stimulated bone resorption and collagenase production by connective tissue cells in vitro","volume":"800","author":"Heath","year":"1984","journal-title":"Biochim Biophys Acta"},{"key":"2024012712001608300_bib5","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1677\/joe.0.1020281","article-title":"Effect of PTH and calcitonin on the cytoplasmic spreading of isolated osteoclasts","volume":"102","author":"Chambers","year":"1984","journal-title":"J Endocrinol"},{"key":"2024012712001608300_bib6","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/BF00218146","article-title":"Experimental study of changes in osteoblastic shape induced by calcitonin and parathyroid extract in an organ culture system","volume":"169","author":"Jones","year":"1976","journal-title":"Cell Tissue Res"},{"key":"2024012712001608300_bib7","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1126\/science.1273593","article-title":"Bone cells in culture: morphologic transformation by hormones","volume":"192","author":"Miller","year":"1976","journal-title":"Science"},{"key":"2024012712001608300_bib8","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0014-4827(83)90070-8","article-title":"A primary role for microfilaments but not microtubules in hormone-induced cytoplasmic retraction","volume":"143","author":"Aubin","year":"1983","journal-title":"Exp Cell Res"},{"key":"2024012712001608300_bib9","first-page":"460","article-title":"Visualization of cytoskeletal elements in rat calvaria and ROS osteoblastic cells: effects of 1,25(OH)2D3 and PTH","volume":"36","author":"Gronowicz","year":"1984","journal-title":"Calcif Tissue Int"},{"key":"2024012712001608300_bib10","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1146\/annurev.ph.47.030185.001433","article-title":"Physiology and pharmacology of gap junctions","volume":"47","author":"Spray","year":"1985","journal-title":"Annu Rev Physiol."},{"key":"2024012712001608300_bib11","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1177\/00220345790580042101","article-title":"Cell-to-cell communication of osteoblasts","volume":"58","author":"Jeansonne","year":"1979","journal-title":"J Dent Res"},{"key":"2024012712001608300_bib12","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/BF02405331","article-title":"Adriamycin inhibits PTH-mediated but not PGE2-mediated stimulation of cAMP formation in isolated bone cells","volume":"36","author":"Kohler","year":"1984","journal-title":"Calcif Tissue Int"},{"key":"2024012712001608300_bib13","first-page":"2681","article-title":"Gap junction structure and cell-to-cell coupling regulation. Is there a calmodulin involvement?","volume":"43","author":"Peracchia","year":"1984","journal-title":"Fed Proc"},{"key":"2024012712001608300_bib14","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0090-6980(75)80076-1","article-title":"Stimulation of bone resorption by various prostaglandins in organ culture","volume":"10","author":"Dietrich","year":"1975","journal-title":"Prostaglandins"},{"key":"2024012712001608300_bib15","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1111\/j.1749-6632.1975.tb19243.x","article-title":"Reciprocal effects of cAMP and cGMP on microtubule-dependent release of lysosomal enzymes","volume":"253","author":"Weissman","year":"1975","journal-title":"Ann NY Acad Sci"},{"key":"2024012712001608300_bib16","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0092-8674(74)90089-0","article-title":"An electron microscope study of the effects of dibutyryl cyclic AMP on Chinese hamster ovary cells","volume":"2","author":"Porter","year":"1974","journal-title":"Cell"},{"key":"2024012712001608300_bib17","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF01870566","article-title":"Hormonal regulation of cell junction permeability: upregulation by catecholamine and PGE2","volume":"70","author":"Radu","year":"1982","journal-title":"J Membr Biol"},{"key":"2024012712001608300_bib18","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev.ph.47.030185.002005","article-title":"Physiological roles of permeable junctions: some possibilities","volume":"47","author":"Sheridan","year":"1985","journal-title":"Annu Rev Physiol"},{"key":"2024012712001608300_bib19","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/BF02409482","article-title":"Morphological evidence of gap junctions between bone cells","volume":"33","author":"Doty","year":"1981","journal-title":"Calcif Tissue Int"},{"key":"2024012712001608300_bib20","first-page":"681","article-title":"Gap junction formation in myometrium: control by estrogen, progesterone, and prostaglandins","volume":"288","author":"Garfield","year":"1980","journal-title":"Am J Physiol"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650010302","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/1\/3\/243\/56429657\/5650010302.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/1\/3\/243\/56429657\/5650010302.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T14:48:37Z","timestamp":1710341317000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/1\/3\/243-249\/7501594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,4,1]]},"references-count":20,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1986,4,1]]},"published-print":{"date-parts":[[1986,4,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650010302","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"type":"print","value":"0884-0431"},{"type":"electronic","value":"1523-4681"}],"subject":[],"published-other":{"date-parts":[[1986,6,1]]},"published":{"date-parts":[[1986,4,1]]}}}