{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:35:55Z","timestamp":1762673755403,"version":"3.37.3"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1986,8,1]],"date-time":"1986-08-01T00:00:00Z","timestamp":523238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of health","doi-asserted-by":"crossref","award":["AM31263"],"award-info":[{"award-number":["AM31263"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1986,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diterpene forskolin which increases 3\u2032,5\u2032-cyclic adenosine monophosphate concentrations (cAMP) in intact cells by directly activating the enzyme adenyl cyclase, was examined for its ability to alter bone resorption in fetal rat long bone cultures. After 48 h, forskolin inhibited resorption at 1.0 and 10 \u03bc However, after 120 h, it had a small stimulatory effect at 1.0 \u03bcM and no net effect on resorption at 10 \u03bc Isobutylmethylxanthine (IBMX), which elevates cAMP levels in cells by inhibiting the enzyme 3\u2032,5\u2032-cyclic adenosine monophosphate phosphodiesterase, produced a resorptive response which was slightly different from that of forskolin. After both 48 and 120 h, IBMX at 0.1 mM stimulated resorption while at 1.0 mM, it had only inhibitory effects. In bones which were stimulated to resorb with either parathyroid hormone or 1,25(OH)2 vitamin D, forskolin inhibited resorption. The inhibitory effects of forskolin on hormonally stimulated resorption were transient in cultures treated with 1.0 \u03bcM but were sustained with 10 \u03bcM. Inhibitory responses to forskolin did not appear to result from toxicity since they were completely reversed when forskolin was removed from the media.<\/jats:p><jats:p>These results imply that agents which increases 3\u2032,5\u2032-cyclic adenosine monophosphate concentrations in bone activate two resorptive pathways: one which is inhibitory and another which is stimulatory.<\/jats:p>","DOI":"10.1002\/jbmr.5650010403","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T18:26:52Z","timestamp":1265221612000},"page":"313-317","source":"Crossref","is-referenced-by-count":15,"title":["Forskolin has both stimulatory and inhibitory effects on bone resorption in fetal rat long bone cultures"],"prefix":"10.1093","volume":"1","author":[{"given":"Joseph A.","family":"Dr. Lorenzo","sequence":"first","affiliation":[{"name":"University of Connecticut Health Center, Farmington, CT 06032"},{"name":"The Division of Endocrinology and Metabolism, The Newington Veterans Administration Medical Center, Newington, CT 06111"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Sousa","sequence":"additional","affiliation":[{"name":"University of Connecticut Health Center, Farmington, CT 06032"},{"name":"The Division of Endocrinology and Metabolism, The Newington Veterans Administration Medical Center, Newington, CT 06111"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy","family":"Quinton","sequence":"additional","affiliation":[{"name":"University of Connecticut Health Center, Farmington, CT 06032"},{"name":"The Division of Endocrinology and Metabolism, The Newington Veterans Administration Medical Center, Newington, CT 06111"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1986,8,1]]},"reference":[{"key":"2024012712013265300_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/BF02408047","article-title":"Cyclic AMP as a second messenger in the skeletal actions of parathyroid hormone: a decade-old hypothesis","volume":"29","author":"Peck","year":"1979","journal-title":"Calcif Tissue Int"},{"key":"2024012712013265300_bib2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1172\/JCI105117","article-title":"Bone resorption in tissue culture: factors influencing the response to parathyroid hormone","volume":"44","author":"Raisz","year":"1965","journal-title":"J Clin Invest"},{"key":"2024012712013265300_bib3","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0090-6980(75)80076-1","article-title":"Stimulation of bone resorption by various prostaglandins in organ culture","volume":"10","author":"Dietrich","year":"1975","journal-title":"Prostaglandins"},{"key":"2024012712013265300_bib4","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1210\/endo-84-4-761","article-title":"Activation of skeletal adenyl cyclase by parathyroid hormone in vitro","volume":"84","author":"Chase","year":"1969","journal-title":"Endocrinology"},{"year":"1981","author":"Martin","key":"2024012712013265300_bib5"},{"volume-title":"I.C.S. 511 Hormonal Control of Calcium Metabolism","author":"Conn","key":"2024012712013265300_bib5"},{"key":"2024012712013265300_bib6","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1126\/science.175.4023.768","article-title":"1,25-dehydroxycholccalciferol, a potent stimulator of bone resorption in tissue culture","volume":"175","author":"Raisz","year":"1972","journal-title":"Science"},{"key":"2024012712013265300_bib7","first-page":"89","article-title":"Cyclic nucleotides in bone and mineral metabolism. 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