{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:25:16Z","timestamp":1760189116211,"version":"3.37.3"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1987,12,1]],"date-time":"1987-12-01T00:00:00Z","timestamp":565315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["(AM19278)"],"award-info":[{"award-number":["(AM19278)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Roxane Laboratories"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Many clinicians continue to prefer dihydrotachysterol (DHT) as the initial vitamin D agent of choice in hypoparathyroidism and renal osteodystrophy because of its long history of efficacy and safety. Assessment of the factors influencing the clinical response to DHT treatment should include measurement of vitamin D metabolite profiles, but investigators have heretofore been unable to measure 1,25(OH)2D because levels have been found to be falsely elevated when employing the chick intestinal cytosol receptor assay. After converting from the chick cytosol receptor assay to the calf thymus receptor assay for measuring 1,25(OH)2D, we did not note falsely elevated levels of 1,25(OH)2D in DHT-treated patients. The design of this study, therefore, was aimed at determining whether or not the calf thymus receptor measured authentic 1,25(OH)2D in such patients. We controlled for the possibility that freezing and thawing or prolonged storage might have either lowered 1,25(OH)2D levels or degraded a metabolite(s) of DHT that would have otherwise been recognized as \u201c1,25(OH)2D\u201d by the calf receptor. Similarly, technical differences between the two assays, source of thymus, and potential interference by other cytosolic proteins were eliminated as causes for the difference between the 1,25(OH)2D levels in the two assays. Our experiments do not provide an explanation for why the thymus receptor does not \u201csee\u201d the interfering metabolite(s) of DHT. This could reflect either a tissue difference or perhaps a species difference. Our results do provide the first opportunity to expand the investigation of the metabolic effects of DHT therapy to include changes in intrinsic 1,25(OH)2D metabolism.<\/jats:p>","DOI":"10.1002\/jbmr.5650020613","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T18:20:25Z","timestamp":1265221225000},"page":"567-570","source":"Crossref","is-referenced-by-count":3,"title":["1,25(OH)2D levels in dihydrotachysterol-treated patients: Influence on 1,25(OH)2D assays"],"prefix":"10.1093","volume":"2","author":[{"given":"Arlene","family":"Taylor","sequence":"first","affiliation":[{"name":"Metabolic Bone Disease Laboratory, Alfred I. DuPont Institute, Wilmington, DE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Norman","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics, Children's Hospital of Philadelphia and Jefferson Medical College, Philadelphia, PA"},{"name":"Metabolic Bone Disease Laboratory, Alfred I. DuPont Institute, Wilmington, DE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1987,12,1]]},"reference":[{"key":"2024012712153279900_bib1","first-page":"1","article-title":"The treatment of renal-glomerular osteodystrophy.","volume":"30","author":"Dent","year":"1961","journal-title":"Quart J Med"},{"key":"2024012712153279900_bib2","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1056\/NEJM196704202761603","article-title":"Comparison between crystalline dihydrotachysterol and calciferol in patients requiring pharmacologic vitamin D therapy.","volume":"276","author":"Harrison","year":"1967","journal-title":"N Engl J Med"},{"key":"2024012712153279900_bib3","first-page":"31","article-title":"Therapy of renal osteodystrophy with dihydrotachysterol in non-dialyzed patients.","volume":"13","author":"Teredesai","year":"1980","journal-title":"Clin Nephrol"},{"key":"2024012712153279900_bib4","first-page":"311","article-title":"The early detection and treatment of renal osteodystrophy.","volume":"5","author":"Cordy","year":"1981","journal-title":"Mineral Electrolyte Metab"},{"key":"2024012712153279900_bib5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.2165\/00003495-198121040-00001","article-title":"Pharmacology and therapeutic use of vitamin D and its analogues.","volume":"21","author":"Pierides","year":"1981","journal-title":"Drugs"},{"key":"2024012712153279900_bib6","first-page":"1031","article-title":"The effects of dihydrotachysterol therapy on the measurement of plasma 1,25(OH)2-vitamin D in humans.","volume":"93","author":"Gray","year":"1979","journal-title":"J Lab Clin Med"},{"key":"2024012712153279900_bib7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1042\/bj1820055","article-title":"Determination of vitamin D and its metabolites in plasma from normal and anephric man.","volume":"182","author":"Shepard","year":"1979","journal-title":"Biochem J"},{"key":"2024012712153279900_bib8","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1210\/jcem-58-1-91","article-title":"A microassay for 1,25-dihydroxyvitamin D not requiring high performance liquid chromatography: application to clinical studies.","volume":"58","author":"Reinhardt","year":"1984","journal-title":"J Clin Endocrinol Metab"},{"key":"2024012712153279900_bib9","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0024-3205(75)90256-8","article-title":"Nuclear and cytoplasmic binding components for vitamin D metabolites.","volume":"16","author":"Brumbaugh","year":"1975","journal-title":"Life Sci"},{"key":"2024012712153279900_bib10","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1210\/jcem-50-1-152","article-title":"Biochemical properties of the 1\u03b1,25-dihydroxyvitamin D3 cytosol receptors from human and chicken intestinal mucosa.","volume":"50","author":"Wecksler","year":"1980","journal-title":"J Clin Endocrinol Metab"},{"key":"2024012712153279900_bib11","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/0006-291X(82)91812-5","article-title":"1,25-Dihydroxyvitamin D3 receptor in bovine thymus gland.","volume":"106","author":"Reinhardt","year":"1982","journal-title":"Biochem Biophys Res Commun"},{"key":"2024012712153279900_bib12","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/S0140-6736(64)90229-6","article-title":"A comparison between A.T. 10 and pure dihydrotachysterol in controlling hypoparathyroidism.","volume":"2","author":"Dent","year":"1964","journal-title":"Lancet"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650020613","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/2\/6\/567\/56429944\/5650020613.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/2\/6\/567\/56429944\/5650020613.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T14:49:14Z","timestamp":1710341354000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/2\/6\/567-570\/7501672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,12,1]]},"references-count":12,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1987,12,1]]},"published-print":{"date-parts":[[1987,12,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650020613","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"type":"print","value":"0884-0431"},{"type":"electronic","value":"1523-4681"}],"subject":[],"published-other":{"date-parts":[[1987,12,1]]},"published":{"date-parts":[[1987,12,1]]}}}