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In every experiment exposure of cells to high medium Ca (2.0\u20132.5 mM) provoked an increased release of both peptides that was highly correlated (r = 0.73). With other test substances, also, changes in both hormones occurred in parallel. The Ca-channel activator, BAY-K-8644 (10 \u03bcM) increased secretion, and this was inhibited by the Ca channel blocker, nitrendipine (10 \u03bcM). The Ca2+ ionophore, ionomycin (5 \u03bcM), increased release, and the calmodulin-Ca channel inhibitor, phenytoin (100 \u03bcM), inhibited Ca-induced release. The active 4\u03b2 isomer of phorbol-12,13-didecanoate (0.1 \u03bcM), but not the inactive 4\u03b1 isomer, increased secretion. The findings suggest that pathways mediating C cell secretion include plasma membrane Ca channels, intracellular [Ca2+], calmodulin, and protein kinase C. The results show that the secretory process in rat C cells involves the release of CGRP as well as CT.<\/jats:p>","DOI":"10.1002\/jbmr.5650040118","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T13:10:10Z","timestamp":1265202610000},"page":"129-134","source":"Crossref","is-referenced-by-count":12,"title":["Cosecretion of calcitonin and calcitonin gene-related peptide from cultured rat medullary thyroid C cells"],"prefix":"10.1093","volume":"4","author":[{"given":"Patricia K.","family":"Seitz","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77550"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cary W.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 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