{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:43:53Z","timestamp":1760121833966},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1990,5,1]],"date-time":"1990-05-01T00:00:00Z","timestamp":641520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We report the presence of reverse transcriptase activity in the supernatant of long-term culture of mononuclear blood cells (monocytes and lymphocytes) isolated from a 27-year-old patient suffering from benign osteopetrosis. The enzyme was purified to homogeneity according to the technique of Chandra and Steel, by chromatography, first on DEAE-cellulose (DE 52) and then on phosphocellulose (P11). After purification, the enzyme was characterized biochemically for its template specificity and ionic requirements. The purified enzyme was able to transcribe poly(rA) \u00b7\u00b7(dT)12-18 and poly(rC) \u00b7\u00b7(dG)12-18 very efficiently and had a marked preference for Mg2+ ions over Mn2+ ions. The pattern of ionic dependency for this enzyme is similar to that of reverse transcriptases purified from human lymphotropic viruses. The patient was tested and found seronegative for HIV-1, HIV-2, and HTLV-I and seropositive (immunoglobulin C ) for cytomegalovirus. Epstein-Barr virus nuclear antigens (EBNA) were detected in the patient's B lymphocytes. Since reverse transcriptase is the hallmark of retroviruses, we suggest that a retrovirus may be involved in the etiology of osteopetrosis.<\/jats:p>","DOI":"10.1002\/jbmr.5650050503","type":"journal-article","created":{"date-parts":[[2010,2,24]],"date-time":"2010-02-24T19:39:36Z","timestamp":1267040376000},"page":"425-435","source":"Crossref","is-referenced-by-count":10,"title":["Retroviral expression in mononuclear blood cells isolated from a patient with osteopetrosis (albers-schonberg disease)"],"prefix":"10.1093","volume":"5","author":[{"given":"L. M.","family":"Labat","sequence":"first","affiliation":[{"name":"CNRS, Institut Biomedical des Cordeliers. Universitk Pierre et Marie Curie. Laboratoire de Physiopathologie Osseuse, 15, rue de l'Ecole de Medecine, 75270 Paris, Cedex 06, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.-F.","family":"Bringuier","sequence":"additional","affiliation":[{"name":"CNRS, Institut Biomedical des Cordeliers. Universitk Pierre et Marie Curie. Laboratoire de Physiopathologie Osseuse, 15, rue de l'Ecole de Medecine, 75270 Paris, Cedex 06, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Chandra","sequence":"additional","affiliation":[{"name":"Klinikum der Johann-Wolfgang-Goethe-Universitat, Gustav-Embden-Zentrum der Biologischen Chemie, Abteilung fur Molecular-biologie Theodor-Stern-Kai 7, 6ooo Frankfurt Main 70, West Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Chandra","sequence":"additional","affiliation":[{"name":"Klinikum der Johann-Wolfgang-Goethe-Universitat, Gustav-Embden-Zentrum der Biologischen Chemie, Abteilung fur Molecular-biologie Theodor-Stern-Kai 7, 6ooo Frankfurt Main 70, West Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. 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