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The mammalian osteopetroses are a pathogenetically heterogeneous group of skeletal disorders characterized by skeletal sclerosis resulting from reduced osteoclast-mediated bone resorption. Using a 51Cr-release microcytotoxicity assay we demonstrated that splenic natural killer (NK) cell activity was significantly reduced in two distinctly different osteopetrotic mutations in the rat, osteopetrosis (op) and toothless (tl). To determine whether this reduction in NK cell-mediated cytotoxicity is caused by decreased cell number and\/or function in these osteopetrotic mutants, we quantitated NK cells by analyzing mononuclear cell suspensions labeled for two-color fluorescence with OX8 and OX 19 monoclonal antibodies in a fluorescence-activated cell sorter. Flow cytometry of these double-labeled cells revealed that the percentage of NK cells (OX8+\/OX19\u2212 subset) in op and tl spleens was not significantly different from that of normal spleens. These results suggest that NK cells in these osteopetrotic mutants are functionally defective. Thus aberrations in osteoclast and NK cell function coexist in these mutations, and their developmental relationships deserve further study.<\/jats:p>","DOI":"10.1002\/jbmr.5650060308","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T17:42:16Z","timestamp":1265218936000},"page":"263-271","source":"Crossref","is-referenced-by-count":13,"title":["Coexistence of reduced function of natural killer cells and osteoclasts in two distinct osteopetrotic mutations in the rat"],"prefix":"10.1093","volume":"6","author":[{"given":"Steven N.","family":"Dr. Popoff","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary E.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Massachusetts School of Medicine, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven B.","family":"Koevary","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Massachusetts School of Medicine, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Sandy C.","family":"Marks","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Massachusetts School of Medicine, Worcester, MA 01655"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1991,3,1]]},"reference":[{"key":"2024012712593168600_bib1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1093\/oxfordjournals.jhered.a108705","article-title":"Three recessive genes for congenital osteopetrosis in the Norway rat","volume":"67","author":"Moutier","year":"1976","journal-title":"J Hered"},{"key":"2024012712593168600_bib2","first-page":"87","article-title":"Osteopetrose 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