{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T10:58:46Z","timestamp":1776855526930,"version":"3.51.2"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1991,6,1]],"date-time":"1991-06-01T00:00:00Z","timestamp":675734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Medical Research and the Natural Sciences"},{"name":"Engineering Research Councils of Canada"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ascorbate (reduced vitamin C) is required for bone formation. We have shown previously that both the osteoblast-like cell line ROS 17\/2.8 and primary cultures of rat calvarial cells possess a saturable, Na+-dependent uptake system for L-ascorbate (J Membr Biol 111:83\u201391, 1989). The purpose of the present study was to investigate the specificity of this transport system for organic anions and its sensitivity to transport inhibitors. Initial rates of ascorbate uptake were measured by incubating ROS 17\/2.8 cells with [L-14C]ascorbate at 37\u00b0C. Uptake of [L-14C]ascorbate (5 \u03bcM) was inhibited 98 \u00b1 1% by coincubation with unlabeled L-ascorbate (3 mM) and 48 \u00b1 4% by salicylate (3 mM), but it was not affected by 3 mM formate, lactate, pyruvate, gluconate, oxalate, malonate, or succinate. Uptake of the radiolabeled vitamin also was not affected by acute (1 minute) exposure of the cells to the Na+ transport inhibitors amiloride and ouabain or the glucose transport inhibitor cytochalasin B. In contrast, anion transport inhibitors rapidly (&amp;lt; 1 minute) and reversibly blocked [L-14C]ascorbate uptake. In order of potency, these drugs were 4,4\u2032-diisothiocyanostilbene-2,2\u2032-disulfonic acid (DIDS) = sulfinpyrazone &amp;gt; furosemide = 4-acetamido-4\u2032-isothiocyanostilbene-2,2\u2032-disulfonic acid (SITS). These findings indicate that the ascorbate transporter is relatively specific for the ascorbate anion, since other organic anions (with the exception of salicylate) did not compete with ascorbate for uptake. Rapid and reversible inhibition by the impermeant antagonists DIDS and SITS suggests that they interact directly with the ascorbate transporter, consistent with location of the transport system in the plasma membrane.<\/jats:p>","DOI":"10.1002\/jbmr.5650060613","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T17:44:29Z","timestamp":1265219069000},"page":"623-629","source":"Crossref","is-referenced-by-count":18,"title":["Ascorbate uptake by ROS 17\/2.8 osteoblast-like cells: Substrate specificity and sensitivity to transport inhibitors"],"prefix":"10.1093","volume":"6","author":[{"given":"S. Jeffrey","family":"Dr. Dixon","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Western Ontario, London, Ontario, Canada, N6A 5C1"},{"name":"Division of Oral Biology, Faculty of Dentistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Kulaga","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N 0W0"},{"name":"Division of Oral Biology, Faculty of Dentistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa M.","family":"Jaworski","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John X.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1991,6,1]]},"reference":[{"issue":"Suppl. 289","key":"2024012712555655500_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1748-1716.1966.tb03661.x","article-title":"Autoradiographic studies on the distribution of C14-labelled ascorbic acid and dehydroascorbic acid","volume":"70","author":"Hammarstr\u00f6m","year":"1966","journal-title":"Acta Physiol Scand"},{"key":"2024012712555655500_bib2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/BF02547284","article-title":"The effects of ascorbic acid deficiency on calcium and collagen metabolism in cultured fetal rat bones","volume":"17","author":"Chen","year":"1975","journal-title":"Calcif Tissue Res"},{"key":"2024012712555655500_bib3","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1007\/BF02619629","article-title":"Ion-transporting ATPases and matrix mineralization in cultured osteoblastlike cells","volume":"20","author":"Anderson","year":"1984","journal-title":"In Vitro"},{"key":"2024012712555655500_bib4","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/BF02556874","article-title":"Mineralized bone nodules formed in vitro from enzymatically released calvaria cell populations","volume":"38","author":"Bellows","year":"1986","journal-title":"Calcif Tissue Int"},{"key":"2024012712555655500_bib5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/jcp.1041430203","article-title":"Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells","volume":"143","author":"Aronow","year":"1990","journal-title":"J Cell Physiol"},{"key":"2024012712555655500_bib6","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0169-6009(89)90061-5","article-title":"Expression of the chondrogenic phenotype by mineralizing cultures of embryonic chick calvarial bone cells","volume":"7","author":"Berry","year":"1989","journal-title":"Bone Miner"},{"key":"2024012712555655500_bib7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1056\/NEJM197907053010104","article-title":"The biosynthesis of collagen and its disorders","volume":"301","author":"Prockop","year":"1979","journal-title":"N Engl J Med"},{"key":"2024012712555655500_bib8","doi-asserted-by":"crossref","first-page":"17281","DOI":"10.1016\/S0021-9258(18)71489-0","article-title":"Ascorbic acid induces alkaline phosphatase, type X collagen, and calcium deposition in cultured chick chondrocytes","volume":"264","author":"Leboy","year":"1989","journal-title":"J Biol Chem"},{"key":"2024012712555655500_bib9","doi-asserted-by":"crossref","first-page":"21346","DOI":"10.1016\/S0021-9258(19)30086-9","article-title":"Induction of mineral deposition by primary cultures of chicken growth plate chondrocytes in ascorbate-containing media. 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