{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:58:55Z","timestamp":1791197935474,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[1991,12,1]],"date-time":"1991-12-01T00:00:00Z","timestamp":691545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AR-18063"],"award-info":[{"award-number":["AR-18063"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["28933"],"award-info":[{"award-number":["28933"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We tested acid and basic fibroblast growth factor (aFGF and bFGF), members of the heparin binding FGF family, for their ability to stimulate bone resorption as measured by the release of previously incorporated 45Ca from cultured fetal rat long bones in the presence and absence of heparin. Purified low-molecular-weight heparin (LMW heparin) at 5\u2013125 \u03bcg\/ml had no direct stimulatory effect. There was little effect from aFGF (10\u221211-10\u22128 M) alone, but increased resorption was observed in the presence of LMW heparin. With bFGF, increased bone resorption was observed at 10\u22129 M but not at 10\u22128 M. The stimulatory effects of aFGF and bFGF in the presence of LMW heparin were not blocked by the addition of indomethacin (10\u22126 M), which blocks prostaglandin production, or hydroxyurea (10\u22123 M), which blocks DNA synthesis. However, pretreatment with aphidicolin (3 \u00d7 10\u22125 M), a potent inhibitor of DNA synthesis, blocked the effect of acid FGF and diminished the effect of bFGF. These results indicate that both aFGF and bFGF can stimulate bone resorption by a prostaglandin-independent mechanism, particularly in the presence of heparin. The activation of FGF-mediated bone resorption by heparin could play a role in producing the osteoporosis that has been described with heparin therapy and mastocytosis.<\/jats:p>","DOI":"10.1002\/jbmr.5650061206","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T17:41:12Z","timestamp":1265218872000},"page":"1301-1305","source":"Crossref","is-referenced-by-count":68,"title":["Effects of acid and basic fibroblast growth factor and heparin on resorption of cultured fetal rat long bones"],"prefix":"10.1093","volume":"6","author":[{"given":"Hollis A.","family":"Simmons","sequence":"first","affiliation":[{"name":"Pfizer Central Research, Groton, Connecticut"},{"name":"Division of Endocrinology and Metabolism, University of Connecticut Health Center, Farmington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence G.","family":"Raisz","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, University of Connecticut Health Center, Farmington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1991,12,1]]},"reference":[{"key":"2024012712572965900_bib1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1210\/edrv-8-2-95","article-title":"Structural characterization and biological functions of fibroblast growth factor","volume":"8","author":"Gospodarowicz","year":"1987","journal-title":"Endocr Rev"},{"key":"2024012712572965900_bib2","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1096\/fasebj.1.6.3315806","article-title":"Fibroblast growth factors","volume":"1","author":"Thomas","year":"1987","journal-title":"FASEB J"},{"key":"2024012712572965900_bib3","first-page":"575","article-title":"The heparin-binding (fibroblast) growth factor family of proteins","volume":"58","author":"Burgess","year":"1989","journal-title":"FASEB J"},{"key":"2024012712572965900_bib4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1172\/JCI112807","article-title":"Effects of endothelial cell growth factor on bone remodelling in vitro","volume":"79","author":"Canalis","year":"1987","journal-title":"J Clin Invest"},{"key":"2024012712572965900_bib5","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1210\/endo-121-6-1917","article-title":"Growth stimulation of rat calvaria osteoblastic cells by acidic fibroblast growth factor","volume":"121","author":"Rodan","year":"1987","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib6","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1172\/JCI113490","article-title":"Effects of basic fibroblast growth factor on bone formation in vitro","volume":"81","author":"Canalis","year":"1988","journal-title":"J Clin Invest"},{"key":"2024012712572965900_bib7","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1210\/endo-123-1-98","article-title":"Regulation of bovine bone cell proliferation by fibroblast growth and transforming growth factor \u03b2","volume":"123","author":"Globus","year":"1988","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib8","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1210\/endo-125-4-2118","article-title":"Effects of fibro-blast-growth factors on deoxyribonucleic acid and collagen synthesis in rat parietal bone cells","volume":"125","author":"McCarthy","year":"1989","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib9","doi-asserted-by":"crossref","first-page":"12665","DOI":"10.1016\/S0021-9258(18)67143-1","article-title":"Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-sepharose","volume":"261","author":"Hauschka","year":"1986","journal-title":"J Biol Chem"},{"key":"2024012712572965900_bib10","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1210\/endo-124-3-1539","article-title":"Cultured bovine bone cells synthesize basic fibroblast growth factor and store it in their extracellular matrix","volume":"124","author":"Globus","year":"1989","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib11","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0169-6009(89)90078-0","article-title":"An acidic fibroblast growth factor stimulates DNA synthesis, inhibits collagen and alkaline phosphatase synthesis and induces resorption in bone","volume":"7","author":"Shen","year":"1989","journal-title":"Bone Miner"},{"key":"2024012712572965900_bib12","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1126\/science.147.3656.407","article-title":"Heparin enhancement of factors stimulating bone resorption in tissue culture","volume":"147","author":"Goldhaber","year":"1965","journal-title":"Science"},{"key":"2024012712572965900_bib13","first-page":"691","article-title":"The stimulating by heparin and parathyroid hormone of bone resorption in tissue culture","volume":"6","author":"Wolinsky","year":"1970","journal-title":"Isr J Med Sci"},{"key":"2024012712572965900_bib14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/BF02555042","article-title":"Effects of heparin on bone formation in cultured fetal rat calvaria","volume":"46","author":"Hurley","year":"1990","journal-title":"Calcif Tissue Int"},{"key":"2024012712572965900_bib15","first-page":"261","article-title":"Heparin osteoporosis: Histomorphometric analysis of bone biopsy","volume":"11","author":"Megard","year":"1982","journal-title":"Nouv Presse Med"},{"key":"2024012712572965900_bib16","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1097\/00003086-198511000-00028","article-title":"The mast cell and bone","volume":"200","author":"McKenna","year":"1985","journal-title":"Clin Orthop"},{"key":"2024012712572965900_bib17","first-page":"960","article-title":"Expression in Escherichia coli of a chemically synthesized gene for biologically active bovine acidic fibroblast growth factor","volume":"5","author":"Linemeyer","year":"1987","journal-title":"Biotechnology"},{"key":"2024012712572965900_bib18","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/0076-6879(91)98012-U","article-title":"Cloning, recombinant expression and characterization of basic fibroblast growth factor","volume":"198","author":"Thompson","year":"1990","journal-title":"Methods Enzymol"},{"key":"2024012712572965900_bib19","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1210\/endo-85-3-446","article-title":"Effect of phosphate, calcium, and magnesium on bone resorption and hormonal responses in tissue culture","volume":"85","author":"Raisz","year":"1969","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib20","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1172\/JCI112517","article-title":"Effects of DNA and prostaglandin synthesis inhibitors on the stimulation of bone resorption by epidermal growth factor in fetal rat long-bone cultures","volume":"77","author":"Lorenzo","year":"1986","journal-title":"J Clin Invest"},{"key":"2024012712572965900_bib21","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1056\/NEJM198512053132305","article-title":"Comparing the means of several groups","volume":"313","author":"Godfrey","year":"1985","journal-title":"N Engl J Med"},{"issue":"Suppl. 2","key":"2024012712572965900_bib22","article-title":"Basic-and acidic fibroblast growth factor stimulate prostaglandin production and bone resorption in neonatal mouse calvaria (abstract 135)","volume":"46","author":"Feyen","year":"1990","journal-title":"Calcif Tissue Int"},{"key":"2024012712572965900_bib23","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/0090-6980(89)90071-3","article-title":"Biphasic effects of nonsteroidal antiinflammatory drugs on prostaglandin production by cultured rat calvariae","volume":"37","author":"Raisz","year":"1989","journal-title":"Prostaglandins"},{"key":"2024012712572965900_bib24","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1210\/endo-123-5-2194","article-title":"Interleukin-1 in combination with transforming growth factor-\u03b1 produces enhanced bone resorption in vitro","volume":"123","author":"Lorenzo","year":"1988","journal-title":"Endocrinology"},{"key":"2024012712572965900_bib25","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/jcp.1041380202","article-title":"Heparin potentiates the action of acidic fibroblast growth factor by prolonging its biological half-life","volume":"138","author":"Damon","year":"1989","journal-title":"J Cell Physiol"},{"key":"2024012712572965900_bib26","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1002\/jcp.1041400306","article-title":"Stabilization by heparin of acidic fibroblast growth factor mitogenicity for human endothelial cells in vitro","volume":"140","author":"Mueller","year":"1989","journal-title":"J Cell Physiol"},{"key":"2024012712572965900_bib27","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1083\/jcb.111.4.1651","article-title":"Heparin and heparan sulfate increase the radius of diffusion and action of basic fibroblast growth factor","volume":"111","author":"Flaumenhaft","year":"1990","journal-title":"J Cell Biol"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650061206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/6\/12\/1301\/56432269\/5650061206.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/6\/12\/1301\/56432269\/5650061206.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T14:53:12Z","timestamp":1710341592000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/6\/12\/1301-1305\/7502269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12,1]]},"references-count":27,"journal-issue":{"issue":"12","published-online":{"date-parts":[[1991,12,1]]},"published-print":{"date-parts":[[1991,12,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650061206","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1991,12,1]]},"published":{"date-parts":[[1991,12,1]]}}}