{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:25:03Z","timestamp":1760189103013},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[1992,8,1]],"date-time":"1992-08-01T00:00:00Z","timestamp":712627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>It is not feasible to use in vivo tetracycline double labeling to study bone formation in biopsies taken during the emergency fixation of fractures. We therefore compared the trabecular localization and extent of osteoblastic alkaline phosphatase (AP) perimeters with tetracycline and osteoid perimeters in iliac crest biopsies from 7 women with postmenopausal osteoporosis and 13 women without metabolic bone disease. Fresh biopsies were chilled to -70\u00b0C, and triplicate serial unfixed undecalcified cryostat sections were cut and reacted for AP, stained for osteoid, or mounted unstained. At individual remodeling sites, the mineralizing perimeter (M.Pm) was measured as the extent of a double or single label accompanied by \u2265 1 lamella of osteoid and \u2265 1 lamella of mineralized matrix between the mineralization front and the adjacent label. Osteoid perimeters (O.Pm) and AP perimeters (AP.Pm) were also measured. In each biopsy there was good agreement between the location of AP and bone formation (x statistic, range 0.71\u20131.0). The overall sensitivity and specificity of AP as an indicator of the location of bone formation were 0.963 and 0.902, respectively. At the level of the basic multicellular unit, in those samples in which &amp;gt;3 active BMUs were found, there was (1) significant positive correlation between the M.Pm and both AP.Pm and AP-positive O.Pm (except 1 patient) and (2) no significant difference between the M.Pm and AP-positive O.Pm (17 of 18 patients and 18 of 18 patients at the tissue level). At the tissue level, the line of regression of AP-positive O.Pm on M.Pm (calculated either as label 2 extent or conventionally as dL + 1\/2sL extent) was not significantly different from the line of identity. The presence of osteoblastic AP activity in biopsies from normal and osteoporotic patients distinguishes osteoid currently contributing to bone formation and should be useful for the study of bone formation in unlabeled bone samples from such individuals. Further studies are necessary to establish the broader applicability of this technique, particularly when a mineralization defect is involved, as in osteomalacia.<\/jats:p>","DOI":"10.1002\/jbmr.5650070807","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T17:09:02Z","timestamp":1265216942000},"page":"905-912","source":"Crossref","is-referenced-by-count":23,"title":["Relationship between the location of osteoblastic alkaline phosphatase activity and bone formation in human iliac crest bone"],"prefix":"10.1093","volume":"7","author":[{"given":"Jeremy N.","family":"Bradbeer","sequence":"first","affiliation":[{"name":"Bone Disease Research Group and Section of Medical Statistics. MRC Clinical Research Centre and Northwick Park Hospital, Middlesex, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan M.","family":"Zanelli","sequence":"additional","affiliation":[{"name":"Bone Disease Research Group and Section of Medical Statistics. MRC Clinical Research Centre and Northwick Park Hospital, Middlesex, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Academic Department of Obstetrics and Gynaecology, Royal Free Hospital, London, England"},{"name":"Bone Disease Research Group and Section of Medical Statistics. MRC Clinical Research Centre and Northwick Park Hospital, Middlesex, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephine","family":"Pearson","sequence":"additional","affiliation":[{"name":"Bone Disease Research Group and Section of Medical Statistics. MRC Clinical Research Centre and Northwick Park Hospital, Middlesex, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Reeve","sequence":"additional","affiliation":[{"name":"Bone Disease Research Group and Section of Medical Statistics. MRC Clinical Research Centre and Northwick Park Hospital, Middlesex, England"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,8,1]]},"reference":[{"key":"2024013113112319500_bib1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1136\/bmj.289.6444.517","article-title":"Impaired osteoblast function in osteoporosis: Comparison between calcium balance and dynamic histomorphometry.","volume":"289","author":"Arlot","year":"1984","journal-title":"Br Med J Clin Res"},{"key":"2024013113112319500_bib2","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1002\/jbmr.5650020604","article-title":"The assessment of bone formation and bone resorption in osteoporosis: A comparison between tetracycline-based iliac histomorphometry and whole body 85Sr kinetics.","volume":"2","author":"Reeve","year":"1987","journal-title":"J Bone Miner Res"},{"key":"2024013113112319500_bib3","first-page":"320","article-title":"Mechanism of mineral formation in bone.","volume":"60","author":"Anderson","year":"1989","journal-title":"Lab Invest"},{"key":"2024013113112319500_bib4","first-page":"175","volume-title":"Bone and Mineral Research 6","author":"Whyte","year":"1989"},{"key":"2024013113112319500_bib5","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF00493318","article-title":"The significance of a differential distribution of phosphomonoesterases on bone surfaces after prolonged demineralisation.","volume":"62","author":"Vilmann","year":"1979","journal-title":"Histochemistry"},{"key":"2024013113112319500_bib6","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1177\/17.12.799","article-title":"Distribution of acid and alkaline phosphatase activity in undemineralised sections of rat tibial diaphysis.","volume":"17","author":"Wergedal","year":"1969","journal-title":"J Histochem Cytochem"},{"key":"2024013113112319500_bib7","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1210\/jcem-66-5-951","article-title":"Concurrent assays of circulating bone gla-protein and bone alkaline phosphatase: Effects of sex, age, and metabolic bone disease.","volume":"66","author":"Duda","year":"1988","journal-title":"J Clin Endocrinol Metab"},{"key":"2024013113112319500_bib8","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/8756-3282(89)90061-6","article-title":"Comparative metabolic enzymatic activity in trabecular as against cortical osteoblasts.","volume":"10","author":"Dodds","year":"1989","journal-title":"Bone"},{"key":"2024013113112319500_bib9","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/BF02555966","article-title":"Histomorphometry of fresh frozen iliac crest bone biopsies.","volume":"44","author":"Carter","year":"1989","journal-title":"Calcif Tissue Int"},{"key":"2024013113112319500_bib10","volume-title":"Statistical Methods for Rates and Proportions","author":"Fleiss","year":"1981"},{"key":"2024013113112319500_bib11","volume-title":"Statistical Methods in Medical Research","author":"Armitage","year":"1987"},{"key":"2024013113112319500_bib12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/8756-3282(85)90047-X","article-title":"A classification of in vivo bone labels after double labeling in canine bones.","volume":"6","author":"Hori","year":"1985","journal-title":"Bone"},{"key":"2024013113112319500_bib13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0221-8747(83)90049-8","article-title":"Identification of the mineralization front: Comparison of a modified toluidine blue stain with tetracycline fluorescence.","volume":"5","author":"Villanueva","year":"1983","journal-title":"Metab Bone Dis"},{"key":"2024013113112319500_bib14","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/BF02557675","article-title":"Relationship between toluidine blue-stained calcification fronts and tetracycline-labeled surfaces in normal human iliac crest biopsies.","volume":"37","author":"Compston","year":"1985","journal-title":"Calcif Tissue Int"},{"key":"2024013113112319500_bib15","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/BF01880415","article-title":"Trabecular and endocortical bone remodelling in postmenopausal osteoporosis: Comparison with normal postmenopausal woman.","volume":"1","author":"Arlot","year":"1990","journal-title":"Osteoporosis Int"},{"issue":"Suppl. 2","key":"2024013113112319500_bib16","article-title":"Frequency distribution of tetracycline based measurements: Implications for the interpretation of bone formation indices in the absence of double labelled surfaces.","volume":"5","author":"Foldes","year":"1990","journal-title":"J Bone Miner Res"},{"key":"2024013113112319500_bib17","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/8756-3282(89)90062-8","article-title":"Label escape theory revisited: The effects of resting periods and section thickness.","volume":"10","author":"Martin","year":"1989","journal-title":"Bone"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650070807","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/7\/8\/905\/56487817\/5650070807.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/7\/8\/905\/56487817\/5650070807.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T14:54:23Z","timestamp":1710341663000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/7\/8\/905-912\/7500658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8,1]]},"references-count":17,"journal-issue":{"issue":"8","published-online":{"date-parts":[[1992,8,1]]},"published-print":{"date-parts":[[1992,8,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650070807","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1992,8,1]]},"published":{"date-parts":[[1992,8,1]]}}}