{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T08:31:21Z","timestamp":1783240281251,"version":"3.54.6"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[1992,9,1]],"date-time":"1992-09-01T00:00:00Z","timestamp":715305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["DK42424"],"award-info":[{"award-number":["DK42424"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["DK45227"],"award-info":[{"award-number":["DK45227"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"name":"St. Louis Shriners Hospital","award":["15952"],"award-info":[{"award-number":["15952"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Deflazacort, a synthetic glucocorticoid reported to have bone-sparing properties in vivo, and Cortisol were compared for their effects on bone formation in vitro. Deflazacort and Cortisol were studied for their effects on DNA and collagen synthesis in cultures of intact fetal rat calvariae and of osteoblast-enriched (Ob) cells from 21- to 22-day-old fetal rat parietal bone. Both steroids were also examined for their effects on skeletal insulin-like growth factor (IGF) I production, which is decreased by Cortisol and appears relevant to its mode of action. After 24 h of culture, deflazacort and Cortisol had limited effects on the parameters studied, although Cortisol at 100 nM decreased [3H]proline incorporation into collagen in intact calvariae. In contrast, after 72 h deflazacort and Cortisol at 1\u2013100 nM inhibited the incorporation of [3H]thymidine into DNA and at 100 nM decreased the incorporation of [3H]proline into collagen and noncollagen protein in intact calvariae. Deflazacort and Cortisol at 10\u20131000 nM decreased calvarial collagen degradation to a similar extent. Both steroids had a similar activity, and at 100 nM for 72 h they decreased IGF-I production by calvariae; however, Cortisol at 10 nM was somewhat more effective than deflazacort in decreasing IGF-I levels. Deflazacort and Cortisol had analogous effects in Ob cell cultures. After 24 h of treatment, deflazacort at 100\u20131000 nM and Cortisol at 10\u20131000 nM decreased the labeling of DNA, and both steroids at 100\u20131000 nM caused a similar decrease in [3H]proline incorporation into collagen in Ob cells. In addition, deflazacort and Cortisol at 100 nM decreased IGF-I levels by about 60% in Ob cell cultures. In conclusion, deflazacort and Cortisol have similar inhibitory actions on bone DNA and collagen synthesis, collagen degradation, and IGF-I concentrations in vitro.<\/jats:p>","DOI":"10.1002\/jbmr.5650070912","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T17:09:36Z","timestamp":1265216976000},"page":"1085-1092","source":"Crossref","is-referenced-by-count":35,"title":["Effects of deflazacort on aspects of bone formation in cultures of intact calvariae and osteoblast-enriched cells"],"prefix":"10.1093","volume":"7","author":[{"given":"Ernesto","family":"Dr. Canalis","sequence":"first","affiliation":[{"name":"Departments of Research, Medicine, and Orthopedics, Saint Francis Hospital and Medical Center, Hartford, Connecticut, and the University of Connecticut School of Medicine, Farmington, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Avioli","sequence":"additional","affiliation":[{"name":"Division of Bone and Mineral Metabolism, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1992,9,1]]},"reference":[{"key":"2024013112175054400_bib1","doi-asserted-by":"crossref","first-page":"352","DOI":"10.7326\/0003-4819-112-5-352","article-title":"Glucocorticoid-induced osteoporosis: Pathogenesis and management","volume":"112","author":"Lukert","year":"1990","journal-title":"Ann Intern Med"},{"key":"2024013112175054400_bib2","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1172\/JCI109506","article-title":"Altered mineral metabolism in glucocorticoid-induced osteopenia: Effect of 25-hydroxyvitamin D administration","volume":"64","author":"Hahn","year":"1979","journal-title":"J Clin Invest"},{"key":"2024013112175054400_bib3","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/BF02405325","article-title":"Effects of prednisone and deflazacort on mineral metabolism and parathyroid hormone activity in humans","volume":"36","author":"Gennari","year":"1984","journal-title":"Calcif Tissue Int"},{"key":"2024013112175054400_bib4","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1210\/endo-112-3-931","article-title":"Effect of glucocorticoids on type I collagen synthesis, alkaline phosphatase activity and deoxyribonucleic acid content in cultured rat calvariae","volume":"112","author":"Canalis","year":"1983","journal-title":"Endocrinology"},{"key":"2024013112175054400_bib5","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1210\/endo-126-3-1569","article-title":"Cortisol inhibits the synthesis of insulin-like growth factor I in skeletal cells","volume":"126","author":"McCarthy","year":"1990","journal-title":"Endocrinology"},{"key":"2024013112175054400_bib6","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1210\/endo-122-1-22","article-title":"Isolation and characterization of insulin-like growth factor I (somatomedin-C) from cultures of fetal rat calvariae","volume":"122","author":"Canalis","year":"1988","journal-title":"Endocrinology"},{"key":"2024013112175054400_bib7","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1210\/endo-124-1-301","article-title":"Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures","volume":"124","author":"McCarthy","year":"1989","journal-title":"Endocrinology"},{"key":"2024013112175054400_bib8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/BF02556669","article-title":"Establishment of the relative antiinflammatory potency of deflazacort and prednisone in polymyalgia rheumatica","volume":"41","author":"Lund","year":"1987","journal-title":"Calcif Tissue Int"},{"key":"2024013112175054400_bib9","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1365-2125.1986.tb05165.x","article-title":"In vitro immunosuppressive potency of deflazacort, a new bone-sparing corticosteroid on T lymphocytes. 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