{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:23:20Z","timestamp":1790511800355,"version":"4.1.0"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1993,1,1]],"date-time":"1993-01-01T00:00:00Z","timestamp":725846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Ministry of Education, Science and Culture of Japan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Severe deficiency of osteoclasts in op\/op mice, caused by the absence of functional macrophage colony-stimulating factor (M-CSF), is cured by daily injections of purified recombinant human M-CSF (rhM-CSF). In this study, we found that a single injection of 5 \u03bcg rhM-CSF is enough for recruitment of osteoclasts in mutant mice. Osteoclast number increased during the period between 2 and 4 days after the single rhM-CSF injection. When YM175, a new derivative of bisphosphonate, was administered to the mice 4 days after rhM-CSF injection or later, osteoclasts disappeared by 3 days after YM175 administration. However, a significant number of osteoclasts were detected even at 3 days after YM175 administration when YM175 was administered 3 days after rhM-CSF injection or earlier. These results indicate that YM175 is cytotoxic only to functioning osteoclasts and that recruitment of osteoclasts is finished 4 days after a single rhM-CSF injection. The osteoclasts actively resorbed bone trabeculae for a prolonged period, demonstrating that M-CSF is not requisite for the functioning of mature osteoclasts.<\/jats:p>","DOI":"10.1002\/jbmr.5650080107","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:44:27Z","timestamp":1265215467000},"page":"45-50","source":"Crossref","is-referenced-by-count":62,"title":["Transient recruitment of osteoclasts and expression of their function in osteopetrotic (<i>op\/op<\/i>) mice by a single injection of macrophage colony-stimulating factor"],"prefix":"10.1093","volume":"8","author":[{"given":"Hiroaki","family":"Kodama","sequence":"first","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ohu University School of Dentistry, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moriaki","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ohu University School of Dentistry, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumpei","family":"Niida","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Fukushima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Hakeda","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy, Meikai University School of Dentistry, Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Biomedical Research Laboratories II, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, 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