{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:02:20Z","timestamp":1791028940519,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1993,2,1]],"date-time":"1993-02-01T00:00:00Z","timestamp":728524800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Imperial Cancer Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study details the investigation of induction of retractile shape change in the osteoclast through inhibition of adhesion between osteoclasts and matrix with (1) peptide analogs bearing an Arg-Gly-Asp (RGD) sequence, (2) antibodies to the integrin \u03b1v\u03b23 vitronectin receptor, and (3) the RGD-containing snake venom peptide echistatin. Osteoclast retraction on dentin has been demonstrated for GRGDSP peptide, in contrast to the inactivity of the analog containing the conservative RGE sequence modification. An osteoclast adhesion assay employing rat or chick bone cells and serum-coated glass coverslips as substrate was developed for routine evaluation of inhibition of adhesion. Antibodies F4 and F11 to the \u03b23 chain of rat vitronectin receptor were effective at submicromolar concentrations in rat osteoclasts (IC50 0.29 and 0.05 \u03bcM, respectively), whereas MAb 23C6 to human\/chick vitronectin receptor was somewhat less effective against chick osteoclasts (ICso 1.6 \u03bcM). A rank order of RGD analog activity (mean IC50, \u03bcM) in the serum-coated glass adhesion assay was derived for the linear peptides GRGDSP (201 \u03bcM), GRGDTP (180 \u03bcM), Ac-RGDS-NH2 (84 \u03bcM), Ac-RGDV-NH2 (68 \u03bcM), RGDV (43 \u03bcM), GRGDS (38 \u03bcM), and RGDS (26 \u03bcM). The two most potent short peptides were the cyclic analog SK&amp;F 106760 Ac-S,S-cyclo-(Cys-(N\u03b1Me)Arg-Gly-Asp-Pen)-NH2 (IC50 7.0 \u03bcM), and the Telios peptide H-Gly-S,S-cyclo-(Pen-Gly-Arg-Gly-Asp-Ser-Pro-Cys)-Ala-OH (IC50 6.6 \u03bcM). The snake venom peptide echistatin was the most potent substance evaluated in the serum-coated glass assay (IC50 0.78 nM) employing either rat or chick osteoclasts. Specificity control peptides fibronectin CS1 (ligand for VLA-4), fibrinogen H12 (alternate ligand for gpIIb\/IIIa), and Iaminin cell binding fragment YIGSR were inactive up to 800 \u03bcM. Comparison of SK&amp;F 106760 and the Telios peptide as inhibitors of platelet aggregation (IC50 0.36 and 10.1 \u03bcM, respectively) and inhibitors of L8 skeletal muscle cell adhesion to vitronectin (IC50 67.2 and 12.3 \u03bcM, respectively) suggests that the Telios peptide is nonselective whereas SK&amp;F 106760 may be selective with regard to \u03b23 integrins. This study demonstrates that structural modification in RGD peptides and the use of antireceptor antibodies or the venom peptide echistatin yields potent inhibitors of vitronectin receptor-mediated adhesion in isolated rat and chick osteoclasts. It is hoped that further peptide modification will yield improved specificity and thus selective inhibitory effects upon bone resorption.<\/jats:p>","DOI":"10.1002\/jbmr.5650080215","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:46:30Z","timestamp":1265215590000},"page":"239-247","source":"Crossref","is-referenced-by-count":65,"title":["Modulation of vitronectin receptor-mediated osteoclast adhesion by Arg-Gly-Asp peptide analogs: A structure-function analysis"],"prefix":"10.1093","volume":"8","author":[{"given":"Michael A.","family":"Horton","sequence":"first","affiliation":[{"name":"ICRF Haemopoiesis Research Group, Department of Haematology, St. Bartholomew's Hospital, London, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine L.","family":"Dorey","sequence":"additional","affiliation":[{"name":"ICRF Haemopoiesis Research Group, Department of Haematology, St. Bartholomew's Hospital, London, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Nesbitt","sequence":"additional","affiliation":[{"name":"ICRF Haemopoiesis Research Group, Department of Haematology, St. Bartholomew's Hospital, London, England"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Samanen","sequence":"additional","affiliation":[{"name":"Department of Peptidomimetic Research, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadia E.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Peptidomimetic Research, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Stadel","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russel","family":"Greig","sequence":"additional","affiliation":[{"name":"Department of Cell Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miep H.","family":"Helfrich","sequence":"additional","affiliation":[{"name":"ICRF Haemopoiesis Research Group, Department of Haematology, St. Bartholomew's Hospital, London, England"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1993,2,1]]},"reference":[{"key":"2024013113121355900_bib1","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1111\/j.1600-0714.1983.tb00337.x","article-title":"The origin of the osteoclast","volume":"12","author":"Marks","year":"1983","journal-title":"J Oral Pathol"},{"key":"2024013113121355900_bib2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1136\/jcp.38.3.241","article-title":"The pathobiology of the osteoclast","volume":"38","author":"Chambers","year":"1985","journal-title":"J Clin Pathol"},{"key":"2024013113121355900_bib3","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1152\/physrev.1986.66.4.855","article-title":"Cells of bone: Proliferation, differentiation and hormonal regulation","volume":"66","author":"Nijweide","year":"1986","journal-title":"Physiol Rev"},{"key":"2024013113121355900_bib4","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1097\/00003086-198806000-00033","article-title":"Cellular biology and biochemical mechanism of bone resorption. A review of recent developments on the formation, activation and mode of action of osteoclasts","volume":"231","author":"Vaes","year":"1988","journal-title":"Clin Orthop"},{"key":"2024013113121355900_bib5","first-page":"177","article-title":"The utlrastructure of the osteoclast and its functional implications","volume":"123","author":"Holtrop","year":"1977","journal-title":"Clin Orthop"},{"key":"2024013113121355900_bib6","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1002\/jbmr.5650060806","article-title":"Kinetics of osteoclast cytoskeleton during the resorption cycle in vitro","volume":"6","author":"Lakkakorpi","year":"1991","journal-title":"J Bone Miner Res"},{"key":"2024013113121355900_bib7","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1083\/jcb.115.4.1179","article-title":"Vitronectin receptor has a role in bone resorption but does not mediate tight sealing zone attachment of osteoclasts to the bone surface","volume":"115","author":"Lakkakorpi","year":"1991","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib8","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0092-8674(87)90233-9","article-title":"Integrins: A family of cell surface receptors","volume":"48","author":"Hynes","year":"1987","journal-title":"Cell"},{"key":"2024013113121355900_bib9","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev.iy.08.040190.002053","article-title":"VLA proteins in the integrin family: Structures, functions, and their role on leukocytes","volume":"8","author":"Hemler","year":"1990","journal-title":"Annu Rev Immunol"},{"key":"2024013113121355900_bib10","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/309030a0","article-title":"Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule","volume":"309","author":"Pierschbacher","year":"1984","journal-title":"Nature"},{"key":"2024013113121355900_bib11","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/0092-8674(86)90259-X","article-title":"Arg-Gly-Asp: A versatile cell recognition signal","volume":"44","author":"Ruoslahti","year":"1986","journal-title":"Cell"},{"key":"2024013113121355900_bib12","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1126\/science.2821619","article-title":"New perspectives in cell adhesion: RGD and integrins","volume":"238","author":"Ruoslahti","year":"1987","journal-title":"Science"},{"key":"2024013113121355900_bib13","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1002\/jbmr.5650040603","article-title":"Perspectives: Adhesion receptors in bone","volume":"4","author":"Horton","year":"1989","journal-title":"J Bone Miner Res"},{"key":"2024013113121355900_bib14","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1083\/jcb.109.3.1321","article-title":"Identification and characterization of the lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin","volume":"109","author":"Wayner","year":"1989","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib15","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1083\/jcb.107.5.1881","article-title":"The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: Preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa","volume":"107","author":"Wayner","year":"1988","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib16","first-page":"741","article-title":"Current status review. Vitronectin receptor: Tissue specific expression or adaptation to culture","volume":"71","author":"Horton","year":"1990","journal-title":"Int J Exp Pathol"},{"key":"2024013113121355900_bib17","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1083\/jcb.109.4.1817","article-title":"The osteoclast functional antigen implicated in the regulation of bone resorption is biochemically related to the vitronectin receptor","volume":"109","author":"Davies","year":"1989","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib18","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1002\/jbmr.5650070315","article-title":"Integrins on rat osteoclasts: Characterisation of two monoclonal antibodies (F4 and Fll) to rat \u03b23","volume":"7","author":"Helfrich","year":"1992","journal-title":"J Bone Miner Res"},{"key":"2024013113121355900_bib19","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0014-4827(91)90386-9","article-title":"Arg-Gly-Asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit cell spreading and dentine resorption by osteoclasts","volume":"135","author":"Horton","year":"1991","journal-title":"Exp Cell Res"},{"issue":"2","key":"2024013113121355900_bib20","first-page":"A5","article-title":"Biochemical and functional characterisation of the chicken osteoclast vitronectin receptor (abstract)","volume":"46","author":"Helfrich","year":"1990","journal-title":"Calcif Tissue Int"},{"key":"2024013113121355900_bib21","first-page":"S154","article-title":"Osteoclast attachment to bone and its subsequent resorption depend upon the vitronectin receptor (abstract)","volume":"5","author":"Alvarez","year":"1990","journal-title":"J Bone Miner Res"},{"key":"2024013113121355900_bib22","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/jbmr.5650070314","article-title":"Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin via a \u03b23 integrin","volume":"7","author":"Helfrich","year":"1992","journal-title":"J Bone Miner Res"},{"key":"2024013113121355900_bib23","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1073\/pnas.87.12.4473","article-title":"Osteopontin \u2014 a possible anchor of osteoclasts to bone","volume":"87","author":"Reinholt","year":"1990","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib24","first-page":"5663","article-title":"Monoclonal antibodies to osteoclastomas (giant cell bone tumours): Definition of osteoclast-specific antigens","volume":"45","author":"Horton","year":"1985","journal-title":"Cancer Res"},{"key":"2024013113121355900_bib25","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1083\/jcb.111.4.1713","article-title":"Echistatin is a potent inhibitor of bone resorption in culture","volume":"111","author":"Sato","year":"1990","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib26","doi-asserted-by":"crossref","first-page":"3114","DOI":"10.1021\/jm00114a022","article-title":"Development of a small RGD peptide fibrinogen receptor antagonist with potent antiaggregatory activity in vitro","volume":"34","author":"Samanen","year":"1991","journal-title":"J Med Chem"},{"key":"2024013113121355900_bib27","first-page":"169","article-title":"An RGD-peptide analog with potent antithrombotic activity in vitro","volume":"14A","author":"Samanen","year":"1990","journal-title":"J Cell Biochem Suppl"},{"key":"2024013113121355900_bib28","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1055\/s-0038-1646556","article-title":"Demonstration of Ac-Arg-Gly-Asp-Ser-NH2 as an antiaggregatory agent in the dog by intracoronary administration","volume":"61","author":"Shebuski","year":"1989","journal-title":"Thromb Haemost"},{"key":"2024013113121355900_bib29","first-page":"95","article-title":"Human osteoclast-specific antigens are expressed in a wide range of non-human species","volume":"67","author":"Horton","year":"1986","journal-title":"Br J Exp Pathol"},{"key":"2024013113121355900_bib30","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1073\/pnas.86.11.4022","article-title":"Chemical synthesis of echistatin, a potent inhibitor of platelet aggregation from Echis carinatus: Synthesis and biological activity of selected analogs","volume":"86","author":"Garsky","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib31","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1073\/pnas.87.7.2471","article-title":"Platelet glycoprotein IIb-IIIa protein antagonists from snake venoms: Evidence for a family of platelet-aggregation inhibitors","volume":"87","author":"Dennis","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib32","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/path.1711360104","article-title":"Calcitonin alters behaviour of isolated osteoclasts","volume":"136","author":"Chambers","year":"1982","journal-title":"J Pathol"},{"key":"2024013113121355900_bib33","doi-asserted-by":"crossref","first-page":"209","DOI":"10.4049\/jimmunol.145.1.209","article-title":"The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor","volume":"145","author":"Biesecker","year":"1990","journal-title":"J Immunol"},{"key":"2024013113121355900_bib34","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0014-4827(85)90173-9","article-title":"Vitronectin \u2014 a major cell attachment promoting protein in fetal bovine serum","volume":"160","author":"Hayman","year":"1985","journal-title":"Exp Cell Res"},{"key":"2024013113121355900_bib35","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1083\/jcb.100.6.1948","article-title":"Detachment of cells from culture substrate by soluble fibronectin peptides","volume":"100","author":"Hayman","year":"1985","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib36","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1016\/S0021-9258(19)39696-6","article-title":"Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin \u03b1\u03b21","volume":"265","author":"Mould","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib37","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/0092-8674(87)90083-3","article-title":"Competition for related but nonidentical binding sites on the glycoprotein IIb-IIa complex by peptides derived from platelet adhesive proteins","volume":"48","author":"Santoro","year":"1987","journal-title":"Cell"},{"key":"2024013113121355900_bib38","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/0092-8674(87)90707-0","article-title":"Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis and receptor binding","volume":"48","author":"Graf","year":"1987","journal-title":"Cell"},{"key":"2024013113121355900_bib39","doi-asserted-by":"crossref","first-page":"16652","DOI":"10.1016\/S0021-9258(19)84755-5","article-title":"The elastin receptor shows structural and functional similarities to the 67-kDa tumor cell laminin receptor","volume":"264","author":"Mecham","year":"1989","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib40","first-page":"127","article-title":"Monoclonal antibodies against osteoclasts inhibit bone resorption in vitro","volume":"1","author":"Chambers","year":"1986","journal-title":"Bone Miner"},{"key":"2024013113121355900_bib41","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/0221-8747(81)90020-5","article-title":"Behavior of osteoclasts during a rapid change in their number induced by high doses of parathyroid hormone or calcitonin in intact rats","volume":"2","author":"Baron","year":"1981","journal-title":"Metab Bone Dis"},{"key":"2024013113121355900_bib42","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1083\/jcb.60.2.346","article-title":"The effects of parathyroid hormone, colchicine and calcitonin on the ultrastructure and the activity of osteoclasts in organ culture","volume":"60","author":"Holtrop","year":"1974","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib43","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0022-5320(72)90017-2","article-title":"Ultrastructural effect of calcitonin in osteoclasts in tissue culture","volume":"39","author":"Kallio","year":"1972","journal-title":"J Ultrastruct Res"},{"key":"2024013113121355900_bib44","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/path.1711450403","article-title":"The effects of calcium regulating hormones on bone resorption by isolated human osteoclastoma cells","volume":"145","author":"Chambers","year":"1985","journal-title":"J Pathol"},{"key":"2024013113121355900_bib45","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.1073\/pnas.80.13.4003","article-title":"Serum spreading factor (vitronectin) is present at the cell surface and in tissues","volume":"80","author":"Hayman","year":"1983","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib46","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1083\/jcb.104.3.585","article-title":"A cell surface receptor complex for collagen type I recognizes the Arg-Gly-Asp sequence","volume":"104","author":"Dedhar","year":"1987","journal-title":"J Cell Biol"},{"key":"2024013113121355900_bib47","doi-asserted-by":"crossref","first-page":"17294","DOI":"10.1016\/S0021-9258(18)45376-8","article-title":"Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion","volume":"262","author":"Pierschbacher","year":"1987","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib48","doi-asserted-by":"crossref","first-page":"4011","DOI":"10.1016\/S0021-9258(19)39695-4","article-title":"Modulation of vitronectin receptor binding by membrane lipid composition","volume":"265","author":"Conforti","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib49","doi-asserted-by":"crossref","first-page":"5766","DOI":"10.1073\/pnas.82.17.5766","article-title":"A 125\/115 kD cell surface receptor specific for vitronectin interacts with the arginine-glycine-aspartic acid adhesion sequence derived from fibronectin","volume":"82","author":"Pytela","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib50","doi-asserted-by":"crossref","first-page":"17703","DOI":"10.1016\/S0021-9258(18)45436-1","article-title":"Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor","volume":"262","author":"Cheresh","year":"1987","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib51","doi-asserted-by":"crossref","first-page":"11008","DOI":"10.1016\/S0021-9258(19)38549-7","article-title":"Purification and functional characterization of integrin \u03b1v\u03b23. An adhesion receptor for vitronectin","volume":"265","author":"Smith","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib52","doi-asserted-by":"crossref","first-page":"17126","DOI":"10.1016\/S0021-9258(17)44878-2","article-title":"cDNA sequence of the human integrin \u03b25 subunit","volume":"265","author":"McLean","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib53","doi-asserted-by":"crossref","first-page":"5415","DOI":"10.1073\/pnas.86.14.5415","article-title":"An alternative cytoplasmic domain of the integrin \u03b23 subunit","volume":"86","author":"van Kuppevelt","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113121355900_bib54","doi-asserted-by":"crossref","first-page":"5938","DOI":"10.1016\/S0021-9258(19)39269-5","article-title":"The integrin \u03b21 subunit associates with the vitronectin receptor \u03b1v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line","volume":"265","author":"Bodary","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib55","doi-asserted-by":"crossref","first-page":"5934","DOI":"10.1016\/S0021-9258(19)39268-3","article-title":"A novel fibronectin receptor with an unexpected sub-unit composition (\u03b1v\u03b21)","volume":"265","author":"Vogel","year":"1990","journal-title":"J Biol Chem"},{"key":"2024013113121355900_bib56","doi-asserted-by":"crossref","first-page":"19650","DOI":"10.1016\/S0021-9258(18)55042-0","article-title":"Cloning and expression of a divergent integrin subunit \u03b23","volume":"266","author":"Moyle","year":"1991","journal-title":"J Biol Chem"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650080215","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/8\/2\/239\/56492709\/5650080215.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/8\/2\/239\/56492709\/5650080215.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T14:55:35Z","timestamp":1710341735000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/8\/2\/239-247\/7500602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":56,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1993,2,1]]},"published-print":{"date-parts":[[1993,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650080215","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1993,2,1]]},"published":{"date-parts":[[1993,2,1]]}}}