{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:48:07Z","timestamp":1770968887234,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1993,4,1]],"date-time":"1993-04-01T00:00:00Z","timestamp":733622400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Recent findings suggest that the vitronectin receptor, a member of the integrin family, plays an important role in the attachment of osteoclasts to bone matrix. We report here the localization by in situ hybridization of the mRNA for the \u03b1 and \u03b2 subunits of the vitronectin receptor in rat bone sections. To generate the rat-specific RNA probes used in this study, we cloned cDNA fragments of integrin chains \u03b1v, \u03b23, and \u03b25 by the polymerase chain reaction from rat cDNA. These fragments share 86\u201391% homology with the respective human sequences. In situ hybridization localized the \u03b1v and \u03b23 mRNAs to regions undergoing extensive bone resorption. The histologic appearance and prestaining of bone sections for tartrate-resistant acid phosphatase (TRAP) indicated the presence of these mRNAs in osteoclasts. These observations support immunohis-tochemical findings that osteoclasts express high levels of the vitronectin receptor, confirm the identity of this receptor as integrin \u03b1v\/\u03b23, and suggest that osteoclasts may actively synthesize these molecules.<\/jats:p>","DOI":"10.1002\/jbmr.5650080404","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:47:47Z","timestamp":1265215667000},"page":"403-414","source":"Crossref","is-referenced-by-count":48,"title":["Expression of \u03b1v and \u03b23 integrin subunits in rat osteoclasts in situ"],"prefix":"10.1093","volume":"8","author":[{"given":"Doron M.","family":"Shinar","sequence":"first","affiliation":[{"name":"Department of Bone Biology and Osteoporosis Research, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azriel","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Bone Biology and Osteoporosis Research, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drora","family":"Halperin","sequence":"additional","affiliation":[{"name":"Tel-Aviv University, School of Dental Medicine, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gideon A.","family":"Rodan","sequence":"additional","affiliation":[{"name":"Department of Bone Biology and Osteoporosis Research, Merck Sharp and Dohme Research Laboratories, West Point, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miron","family":"Weinreb","sequence":"additional","affiliation":[{"name":"Tel-Aviv University, School of Dental Medicine, 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