{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:05:35Z","timestamp":1710374735551},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1993,4,1]],"date-time":"1993-04-01T00:00:00Z","timestamp":733622400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"NIH","award":["AM 01244"],"award-info":[{"award-number":["AM 01244"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In Adult Cancellous Bone, Cyclic Remodeling Occurs In Discrete Locations (Bmus, Or Basic Multicellular Units). Some Aspects Of These Remodeling Cycles Are Still Not Clear. Only About Half The Osteoid Surface Normally Has Tetracycline Labels; The Remainder Is Thought To Be Immature Or In A Resting Phase. This Study Was Designed To Test The Assumption That Pauses Occur During The Bone Formation Periods. Iliac Crest Bone Biopsies Were Performed In 84 Women With Postmenopausal Osteoporosis. The Subjects Were Given Three Tetracycline Labels; The Middle Label Had A Distinctive Color. The Osteoid Surface Was 10 s\u00b1 6% Of The Bone Surface; The Range Was 0.6\u201324.4%. The Mineralizing Surface Was 42 \u00b1 26% Of The Osteoid Surface. Immature Osteoid Was 7.6 \u00b1 5.3% Of The Osteoid Surface. There Were No Bmus With The First And Third Tetracycline Label But Without The Middle Demeclocycline Label. The Expected Length Of Double 1\u20133 Label, Based On An Assumption That Unlabeled Mature Osteoid Was Temporarily In A Resting Phase, Was Significantly Higher Than The Observed Length (68 \u00b1 77 \u03bcm\/Mm2 tissue area versus 0, p &amp;lt; 0.0001). The mean active formation period was 77 \u00b1 16 days calculated by standard methods but was only 55 \u00b1 26 days when calculated by label escape (p &amp;lt; 0.001). The correlation coefficient between these two methods was only modest (r = 0.27, p &amp;lt; 0.01). The failure to find any double 1\u20133 labels despite abundant triple labels provides strong evidence against the presence of pauses in bone mineralization between 3 and 34 days. Adjusting the formation period or the mineral apposition rate by the mineralizing surface\/osteoid surface ratio may not be valid in women with osteoporosis.<\/jats:p>","DOI":"10.1002\/jbmr.5650080408","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:47:47Z","timestamp":1265215667000},"page":"443-450","source":"Crossref","is-referenced-by-count":10,"title":["Bone formation periods studied with triple tetracycline labels in women with postmenopausal osteoporosis"],"prefix":"10.1093","volume":"8","author":[{"given":"Susan M.","family":"Ott","sequence":"first","affiliation":[{"name":"Department Of Medicine, University Of Washington, Seattle"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1993,4,1]]},"reference":[{"key":"2024013113115974700_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0221-8747(82)90002-9","article-title":"The coupling of bone formation to bone resorption: A critical analysis of the concept and its relevance to the pathogenesis of osteoporosis","volume":"4","author":"Parfitt","year":"1982","journal-title":"Metab Bone Dis"},{"key":"2024013113115974700_bib2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/jbmr.5650030203","article-title":"Static and tetracycline-based bone histomorphometric data from 34 normal postmenopausal females","volume":"3","author":"Recker","year":"1988","journal-title":"J Bone Miner Res"},{"key":"2024013113115974700_bib3","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/jbmr.5650020617","article-title":"Bone histomorphometry: Standardization of nomenclature, symbols, and units","volume":"2","author":"Parfitt","year":"1987","journal-title":"J Bone Miner Res"},{"key":"2024013113115974700_bib4","first-page":"167","article-title":"Resting seams: \u201cOn\u201d and \u201cOff\u201d in lamellar bone formation centers","volume":"2S","author":"Frost","year":"1980","journal-title":"Metab Bone Dis"},{"key":"2024013113115974700_bib5","first-page":"133","volume-title":"Bone Histomorphometry: Techniques and Interpretation","author":"Frost","year":"1983"},{"key":"2024013113115974700_bib6","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/8756-3282(85)90047-X","article-title":"A classification of in vivo bone labels after double labeling in canine bones","volume":"6","author":"Hori","year":"1985","journal-title":"Bone"},{"key":"2024013113115974700_bib7","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/8756-3282(89)90062-8","article-title":"Label escape theory revisited: The effects of resting periods and section thickness","volume":"10","author":"Martin","year":"1989","journal-title":"Bone"},{"key":"2024013113115974700_bib8","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0221-8747(82)90033-9","article-title":"The label escape error: Determination of the active bone-forming surface in histologic sections of bone measured by tetracycline double labels","volume":"4","author":"Schwartz","year":"1982","journal-title":"Metab Bone Dis"},{"key":"2024013113115974700_bib9","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/BF02408616","article-title":"Tetracycline labeling of bone in vivo","volume":"30","author":"Tam","year":"1980","journal-title":"Calcif Tissue Int"},{"key":"2024013113115974700_bib10","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1002\/jbmr.5650020604","article-title":"The assessment of bone formation and bone resorption in osteoporosis. 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