{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:56:26Z","timestamp":1791305786762,"version":"4.3.1"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1994,3,1]],"date-time":"1994-03-01T00:00:00Z","timestamp":762480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In many cell systems, cell-cell and cell-matrix interactions are mediated by integrins, a family of cell surface heterodimeric glycoprotein receptors. Osteoclast integrins may play a role in the process of bone resorption. Osteoclasts express the \u03b1v and \u03b23 subunits of the vitronectin receptor and adhere to a wide range of proteins in vitro, all of which contain the amino acid sequence Arg-Gly-Asp (RGD), an adhesion site recognition sequence common to many protein ligands that bind to integrins. The effect of kistrin, an RGD-containing snake venom protein, on osteoclast-mediated bone resorption was investigated in vivo and in vitro. When kistrin was infused into normocalcemic and hypercalcemic mice, serum calcium was significantly lowered at 3 and 6 h after the start of infusion, indicating an inhibitory effect on osteoclast activity in vivo. In vitro, kistrin potently inhibited bone resorption by isolated rat osteoclasts cultured on slices of bovine bone, and kistrin also inhibited the attachment of 293 cells expressing recombinant human \u03b1v\u03b23 to fibrinogen (IC50 = 1 nM). These results indicate the potential therapeutic use of RGD-containing molecules for hypercalcemia of malignancy or for other disorders associated with bone loss.<\/jats:p>","DOI":"10.1002\/jbmr.5650090313","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:30:05Z","timestamp":1265214605000},"page":"381-387","source":"Crossref","is-referenced-by-count":37,"title":["Effects of kistrin on bone resorption in vitro and serum calcium in vivo"],"prefix":"10.1093","volume":"9","author":[{"given":"Kathleen L.","family":"King","sequence":"first","affiliation":[{"name":"Department of Cell Analysis, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"D'Anza","sequence":"additional","affiliation":[{"name":"Endocrinology Section and Nuclear Medicine Service, Veterans Administration Medical Center, Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Bodary","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Pitti","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Research, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Siegel","sequence":"additional","affiliation":[{"name":"Department of Preclinical Chemistry, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Lazarus","sequence":"additional","affiliation":[{"name":"Department of Protein Engineering, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Dennis","sequence":"additional","affiliation":[{"name":"Department of Protein Engineering, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"JR.","given":"R. Glenn","family":"Hammonds","sequence":"additional","affiliation":[{"name":"Department of Protein Chemistry, Genentech, Inc., South San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhash C.","family":"Kukreja","sequence":"additional","affiliation":[{"name":"Endocrinology Section and Nuclear Medicine Service, Veterans Administration Medical Center, Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1994,3,1]]},"reference":[{"key":"2024013113130235200_bib1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0092-8674(87)90233-9","article-title":"Integrins: A family of cell surface receptors","volume":"48","author":"Hynes","year":"1987","journal-title":"Cell"},{"key":"2024013113130235200_bib2","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1083\/jcb.109.4.1817","article-title":"The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor","volume":"109","author":"Davies","year":"1989","journal-title":"J Cell Biol"},{"key":"2024013113130235200_bib3","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0014-4827(91)90386-9","article-title":"Arg-gly-asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit dentine resorption and cell spreading by osteoclasts","volume":"195","author":"Horton","year":"1991","journal-title":"Exp Cell Res"},{"key":"2024013113130235200_bib4","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/jbmr.5650070314","article-title":"Rat osteoclasts adhere to a wide range of RGD (arg-gly-asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a \u03b23 integrin","volume":"7","author":"Helfrich","year":"1992","journal-title":"J Bone Miner Res"},{"key":"2024013113130235200_bib5","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1126\/science.2821619","article-title":"New perspectives in cell adhesion: RGD and integrins","volume":"238","author":"Ruoslahti","year":"1987","journal-title":"Science"},{"key":"2024013113130235200_bib6","doi-asserted-by":"crossref","first-page":"168","DOI":"10.3181\/00379727-195-43129B","article-title":"Disintegrins: A family of integrin inhibitor proteins from viper venoms","volume":"195","author":"Gould","year":"1990","journal-title":"Proc Soc Exp Biol Med"},{"key":"2024013113130235200_bib7","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1073\/pnas.87.7.2471","article-title":"Platelet glycoprotein IIb-IIIa protein antagonists from snake venoms: Evidence for a family of platelet-aggregation inhibitors","volume":"87","author":"Dennis","year":"1990","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113130235200_bib8","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1083\/jcb.111.4.1713","article-title":"Echistatin is a potent inhibitor of bone resorption in culture","volume":"111","author":"Sato","year":"1990","journal-title":"J Cell Biol"},{"key":"2024013113130235200_bib9","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1161\/01.CIR.83.3.1038","article-title":"Kistrin, a polypeptide platelet GPIIb\/IIIa receptor antagonist, enhances and sustains coronary arterial thromobolysis with recombinant tissue-type plasminogen activator in a canine preparation","volume":"83","author":"Yasuda","year":"1991","journal-title":"Circulation"},{"key":"2024013113130235200_bib10","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1210\/endo.132.3.8440195","article-title":"Inhibition of osteoclastic bone resorption in vivo by echistatin, an \u201carginyl-glycyl-aspartyl\u201d (RGD)-containing protein","volume":"132","author":"Fisher","year":"1993","journal-title":"Endocrinology"},{"key":"2024013113130235200_bib11","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1210\/endo-129-4-1762","article-title":"A carboxyl-terminal peptide from the parathyroid hormone-related protein inhibits bone resorption by osteoclasts","volume":"129","author":"Fenton","year":"1991","journal-title":"Endocrinology"},{"key":"2024013113130235200_bib12","author":"Bodary"},{"key":"2024013113130235200_bib13","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0022-1759(84)90477-0","article-title":"Measurement of cell numbers by means of endogenous enzyme hexosaminidase. 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