{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:27:09Z","timestamp":1757705229054},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[1994,11,1]],"date-time":"1994-11-01T00:00:00Z","timestamp":783648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Glaxo Group Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1994,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Monoclonal antibodies (MAb) may provide valuable tools for studying osteoblast differentiation. We therefore raised a panel of MAb reactive with cells of this phenotype using 1,25(OH)2D3-treated human trabecular osteoblast-like cells (HOBS) as the immunogen. Immunohistochemical studies on various tissues, including undecalcified cryostat sections of fetal and adult human bone, identified 11 bone cell-reactive MAb. Of these, 2 demonstrated particularly selective reactivities against osteocytes (OB\/M) and osteoblasts (OB\/L). These reactivities were also seen in developing bone from rat, rabbit, and marmoset. OB\/L and OB\/M demonstrated limited reactivity against a small number of human tissues from the extensive panel of substrates tested. Both MAb exhibited reactivity against discrete populations of cells in the large and small intestine. In addition, OB\/L reacted with cells in the basal epidermis of skin and OB\/M with cells in blood vessel walls. Both antibodies demonstrated reactivity against a variety of cultured osteoblast-like cell lines and other cultured cell types. These MAb may therefore provide a valuable means of studying osteoblast ontogeny.<\/jats:p>","DOI":"10.1002\/jbmr.5650091104","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:26:55Z","timestamp":1265214415000},"page":"1687-1696","source":"Crossref","is-referenced-by-count":19,"title":["Monoclonal antibodies with selective reactivity against osteoblasts and osteocytes in human bone"],"prefix":"10.1093","volume":"9","author":[{"given":"Susan","family":"Walsh","sequence":"first","affiliation":[{"name":"Bath Institute for Rheumatic Diseases, and University of Bath, Bath, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Dodds","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"},{"name":"Bath Institute for Rheumatic Diseases, and University of Bath, Bath, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian E.","family":"James","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"},{"name":"Bath Institute for Rheumatic Diseases, and University of Bath, Bath, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy N.","family":"Bradbeer","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"},{"name":"Bone Disease Research Group, MRC Clinical Research Centre, Harrow, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxine","family":"Gowen","sequence":"additional","affiliation":[{"name":"Department of Cellular Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania"},{"name":"Bath Institute for Rheumatic Diseases, and University of Bath, Bath, 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