{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:32:32Z","timestamp":1770503552619,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1995,6,1]],"date-time":"1995-06-01T00:00:00Z","timestamp":801964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Estrogen deficiency in rats is responsible for increased osteoclastic resorption and a subsequent rapid bone loss. TGF-\u03b2, which is known to have acute effects on bone resorption in several in vitro models, has been shown to be secreted by osteoblastic cells in vitro in response to 17\u03b2-estradiol, but little is known about its in vivo effects on bone resorption. We therefore decided to investigate the short-term effect of TGF-\u03b21 on bone resorption in ovariectomized rats. TGF-\u03b21 (0.04\u201320 ng\/injection), or vehicle, was injected daily directly into the bone marrow space, through a thin catheter implanted in the distal end of the right femur, during 4 consecutive days, starting 14 days after the ovariectomy. Bone histomorphometry was performed in the secondary spongiosa of the metaphysis of injected femurs and compared with vehicle-injected femurs of sham ovariectomized rats. Ovariectomy was associated with a marked increase in the resorption surface, a 2-fold increase in the number of osteoclasts, and no change in the number of TRAP-positive marrow cells distant from bone surfaces. Bone resorption was significantly lower in the TGF-\u03b21-injected bones of ovariectomized rats, as compared with vehicle injected bones: the osteoclast surface and the number of osteoclasts were, respectively, 11.0 \u00b1 5.1% versus 20.8 \u00b1 1.3% and 287 \u00b1 41 versus 505 \u00b1 53, in bones injected with 0.2 ng of TGF-\u03b21 as compared with vehicle-injected bones (mean \u00b1 SE, p &amp;lt; 0.05). The bone formation rate, assessed by double tetracycline labeling, was increased in ovariectomized rats, but was significantly decreased in TGF-\u03b21-injected bones (2 and 20 ng\/injection) of ovariectomized rats. The trabecular bone volume was decreased in ovariectomized animals, but was not modified in TGF-\u03b21-injected bones in this short-term experiment. We conclude that, when injected into bones of ovariectomized rats, TGF-\u03b21 attenuates the stimulation of osteoclastic resorption induced by estrogen deficiency. These data therefore suggest that a local failure of TGF-\u03b2 secretion could be partly responsible for the stimulation of bone resorption and subsequent bone loss due to the cessation of the ovarian secretion.<\/jats:p>","DOI":"10.1002\/jbmr.5650100619","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:08:42Z","timestamp":1265213322000},"page":"971-977","source":"Crossref","is-referenced-by-count":34,"title":["Short-term local injections of transforming growth factor-\u03b21 decrease ovariectomy-stimulated osteoclastic resorption in vivo in rats"],"prefix":"10.1093","volume":"10","author":[{"given":"Johann","family":"Beaudreuil","sequence":"first","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. F75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Mbalaviele","sequence":"additional","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. F75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Cohen-Solal","sequence":"additional","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. F75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Morieux","sequence":"additional","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. F75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie Christine","family":"de Vernejoul","sequence":"additional","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. F75010 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Orcel","sequence":"additional","affiliation":[{"name":"Inserm Unit\u00e9 349, Cell and Molecular Biology of Bone and Cartilage, Centre Viggo Petersen, H\u01d2pital Lariboisi\u00e8re, 6 rue Guy Patin. 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