{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T21:08:05Z","timestamp":1774645685616,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[1995,9,1]],"date-time":"1995-09-01T00:00:00Z","timestamp":809913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Institute of Health","award":["AR-27032"],"award-info":[{"award-number":["AR-27032"]}]},{"name":"National Institute of Health","award":["AR39737"],"award-info":[{"award-number":["AR39737"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Whether renal phosphate wasting in X-linked hypophosphatemia (XLH) results from an intrinsic renal or humoral defect remains controversial. In studies of the murine homolog of XLH, harboring the Simian Virus 40 (SV40) large T antigen, we obviated the influence of renal cell heterogeneity and impressed memory by comparing Na+-phosphate cotransport in immortalized cells from the S1 segment of the proximal tubule. Cells from SV40 transgenic normal and Hyp mice exhibit characteristics of differentiated proximal tubule cells including gluconeogenesis and alkaline phosphatase activity. Surprisingly, however, we found two distinct populations of cells from the S1 proximal tubule of both normal and Hyp mice. In one, PTH treatment increases cAMP accumulation, while in the other both PTH and thyrocalcitonin enhance cAMP production. Kinetic parameters for Na+-phosphate cotransport were similar in both subpopulations of cells from normal (Km, 0.29 \u00b1 0.03 vs. 0.39 \u00b1 0.04 mM; Vmax, 4.6 \u00b1 0.6 vs. 5.2 \u00b1 0.4 nmol\/mg\/5 minutes) and Hyp mice (0.33 \u00b1 0.02 vs. 0.26 \u00b1 0.04; 6.0 \u00b1 0.7, 4.8 \u00b1 0.6). More importantly, phosphate transport in S1 cells of either subpopulation from Hyp mice is no different than that of normals. These data indicate that renal proximal tubule cells from Hyp mice have intrinsically normal phosphate transport and support the hypothesis that a humoral abnormality underlies renal phosphate wasting in XLH.<\/jats:p>","DOI":"10.1002\/jbmr.5650100909","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:12:08Z","timestamp":1265213528000},"page":"1327-1333","source":"Crossref","is-referenced-by-count":39,"title":["Phosphate transport in immortalized cell cultures from the renal proximal tubule of normal and <i>Hyp<\/i> Mice: Evidence That the <i>HYP<\/i> gene locus product is an extrarenal factor"],"prefix":"10.1093","volume":"10","author":[{"given":"Teresa","family":"Dr. Nesbitt","sequence":"first","affiliation":[{"name":"Departments of Medicine and Cell Biology and The Sarah W. Stedman Nutrition Center, Duke University Medical Center, Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Econs","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Cell Biology and The Sarah W. Stedman Nutrition Center, Duke University Medical Center, Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong K.","family":"Byun","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Cell Biology and The Sarah W. Stedman Nutrition Center, Duke University Medical Center, Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9e","family":"Martel","sequence":"additional","affiliation":[{"name":"MRC Genetics Group, Department of Pediatrics, McGill University-Montreal Children's Hospital Research Institute, Quehec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harriet S.","family":"Tenenhouse","sequence":"additional","affiliation":[{"name":"MRC Genetics Group, Department of Pediatrics, McGill University-Montreal Children's Hospital Research Institute, Quehec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc K.","family":"Drezner","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Cell Biology and The Sarah W. Stedman Nutrition Center, Duke University Medical Center, Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1995,9,1]]},"reference":[{"key":"2024013113135970100_bib1","first-page":"3717","volume-title":"The Metabolic and Molecular Basis of Inherited Disease","author":"Rasmussen","year":"1995"},{"key":"2024013113135970100_bib2","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.1073\/pnas.73.12.4667","article-title":"Hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin D-resistant) rickets.","volume":"73","author":"Eicher","year":"1976","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024013113135970100_bib3","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1038\/ki.1978.115","article-title":"Renal handling of phosphate in vivo and in vitro by the X-linked hypophosphatemic male mouse: evidence for a defect in the 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