{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:53:06Z","timestamp":1790679186727,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[1995,11,1]],"date-time":"1995-11-01T00:00:00Z","timestamp":815184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AR 39870 (JRS)"],"award-info":[{"award-number":["AR 39870 (JRS)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AR 42358 (NSF)"],"award-info":[{"award-number":["AR 42358 (NSF)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Clinical studies indicate that as a group, osteogenesis imperfecta (OI) subjects are shorter than age- and sex-matched controls. Not only somatic growth, but also cellular growth appears to be impaired, and these may be related to defects in extracellular matrix common to this disorder. We have investigated the growth characteristics of dermal fibroblasts and trabecular osteoblasts isolated from patients with OI and control subjects of various ages. Cell growth curves and cell doubling times were determined by measuring cell number using crystal violet dye binding. Growth curves were modeled by a modified logistic function, the three parameters of which are markers for biologically relevant growth parameters: the plateau value or upper asymptote, which reflects the maximum cell density upon confluence; the maximal growth rate (\u03bcm); and the lag time. Both normal human fibroblasts and osteoblasts showed an age-dependent decrease in \u03bcm. Normal fibroblasts exhibited no age-dependence to their upper asymptote or lag time. Fibroblasts derived from patients with OI did not have significantly different upper asymptote values, \u03bcm, or lag times when compared with normal fibroblasts. Normal osteoblasts had a decrease in upper asymptote, decrease in \u03bcm, but a relatively constant lag time with increasing age. In contrast, OI osteoblast \u03bcm was decreased relative to that of normal subjects. For osteoblasts from OI patients, decreased \u03bcm appeared unrelated to the age of the subject, whereas OI fibroblasts did exhibit an age-dependent decrease in \u03bcm. The percentage of collagenase-digestible protein (a measure of collagen synthesis) produced by normal human fibroblasts correlated well with \u03bcm. Treating normal human osteoblasts with the proline analogue 3,4-dehydroproline, which destabilizes collagen triple helix formation and alters collagen synthesis, secretion, and turnover, also decreased \u03bcm. A dose response to varying concentrations of 3,4-dehydroproline was observed for normal human bone cell \u03bcm. These data suggest a link between type I collagen synthesis and cellular proliferation.<\/jats:p>","DOI":"10.1002\/jbmr.5650101113","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T16:02:12Z","timestamp":1265212932000},"page":"1705-1712","source":"Crossref","is-referenced-by-count":50,"title":["Cell proliferation of human fibroblasts and osteoblasts in osteogenesis imperfecta: Influence of age"],"prefix":"10.1093","volume":"10","author":[{"given":"N.S.","family":"Fedarko","sequence":"first","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"D'Avis","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.R.","family":"Frazier","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.J.","family":"Burrill","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Fergusson","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Tayback","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.D.","family":"Sponseller","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.R.","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Bone Metabolism Research Laboratory, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1995,11,1]]},"reference":[{"key":"2024013114210053400_bib1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0168-9525(90)90235-X","article-title":"Brittle bones\u2014fragile molecules: disorders of the collagen gene structure and expression.","volume":"6","author":"Byers","year":"1990","journal-title":"Trends Genet"},{"key":"2024013114210053400_bib2","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/348018a0","article-title":"Bone disease cracks genetics.","volume":"348","author":"Sykes","year":"1990","journal-title":"Nature"},{"key":"2024013114210053400_bib3","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1002\/jbmr.5650070809","article-title":"Extracellular matrix formation by osteoblasts from patients with osteogenesis imperfecta.","volume":"1992","author":"Fedarko","year":"1992","journal-title":"J Bone Miner Res"},{"key":"2024013114210053400_bib4","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1002\/ajmg.1320450223","article-title":"Evaluation of growth hormone axis and responsiveness to growth stimulation of short children with osteogenesis imperfecta.","volume":"45","author":"Marini","year":"1993","journal-title":"Am J Med Genet"},{"key":"2024013114210053400_bib5","first-page":"251","article-title":"The growth hormone and somatomedin axis in short children with osteogenesis imperfecta.","volume":"76","author":"Marini","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"2024013114210053400_bib6","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1111\/j.1651-2227.1993.tb12554.x","article-title":"Serum concentrations of procollagen I C-terminal propeptide, osteocalcin and insulin-like growth factor-I in patients with non-lethal osteogenesis imperfecta.","volume":"82","author":"Brenner","year":"1993","journal-title":"Acta Paediatr"},{"key":"2024013114210053400_bib7","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1172\/JCI115622","article-title":"An osteopenic nonfracture syndrome with features of mild osteogenesis imperfecta associated with the substitution of a cysteine for glycine at triple helix position 43 in the proalphal(I) chain of type I collagen?","volume":"89","author":"Shapiro","year":"1992","journal-title":"J Clin Invest"},{"key":"2024013114210053400_bib8","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/BF02557826","article-title":"Human bone cells in culture.","volume":"37","author":"Robey","year":"1985","journal-title":"Calcif Tissue Int"},{"key":"2024013114210053400_bib9","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/0003-2697(90)90454-H","article-title":"Computerized determination of growth curves and doubling times from cells in microculture.","volume":"187","author":"Reile","year":"1990","journal-title":"Anal Biochem"},{"key":"2024013114210053400_bib10","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1128\/aem.56.6.1875-1881.1990","article-title":"Modeling of bacterial growth curves.","volume":"56","author":"Zweitering","year":"1990","journal-title":"Appl Environ Microbiol"},{"key":"2024013114210053400_bib11","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1021\/bi00782a009","article-title":"Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.","volume":"10","author":"Peterkofsky","year":"1971","journal-title":"Biochemistry"},{"key":"2024013114210053400_bib12","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1083\/jcb.102.2.587","article-title":"A unique heparan sulfate in the nuclei of hepatocytes: structural changes with growth state of the cells.","volume":"102","author":"Fedarko","year":"1986","journal-title":"J Cell Biol"},{"key":"2024013114210053400_bib13","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1136\/jmg.16.2.101","article-title":"Genetic heterogeneity in osteogenesis imperfecta.","volume":"16","author":"Sillence","year":"1978","journal-title":"J Med Genet"},{"key":"2024013114210053400_bib14","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1002\/jcp.1041510202","article-title":"Age-related changes in hyaluronan, proteoglycan, collagen and osteonectin synthesis by human bone cells.","volume":"151","author":"Fedarko","year":"1992","journal-title":"J Cell Physiol"},{"key":"2024013114210053400_bib15","first-page":"591","article-title":"Recurrence of lethal osteogenesis imperfecta due to parental mosaicism for a dominant mutation in a human type I collagen gene (COLIA1).","volume":"40","author":"Cohn","year":"1990","journal-title":"Am J Hum Genet"},{"key":"2024013114210053400_bib16","first-page":"S226","article-title":"Bone histomorphometric analysis in osteogenesis imperfecta.","volume":"9","author":"Glorieux","year":"1994","journal-title":"J Bone Miner Res"},{"key":"2024013114210053400_bib17","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2106\/00004623-197355020-00003","article-title":"Osteogenesis imperfecta: a histometric analysis.","volume":"55A","author":"Falvo","year":"1973","journal-title":"J Bone Joint Surg"},{"key":"2024013114210053400_bib18","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1097\/00003086-197110000-00027","article-title":"Electron microscopic and histochemical investigation of osteogenesis imperfecta tarda.","volume":"80","author":"Doty","year":"1971","journal-title":"Clin Orthop Rel Res"},{"key":"2024013114210053400_bib19","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1096\/fasebj.4.13.2210157","article-title":"Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation.","volume":"4","author":"Stein","year":"1990","journal-title":"FASEB J"},{"key":"2024013114210053400_bib20","first-page":"241","volume-title":"Disorders of Bone and Mineral Metabolism","author":"Robey","year":"1991"},{"key":"2024013114210053400_bib21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/978-1-4684-1821-7_2","volume-title":"Aging in Cells and Tissue Culture","author":"Hay","year":"1970"},{"key":"2024013114210053400_bib22","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-1-4684-1821-7_7","volume-title":"Aging in Cells and Tissue Culture","author":"Maciera-Coelhe","year":"1970"},{"key":"2024013114210053400_bib23","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1083\/jcb.105.1.457","article-title":"Osteoblasts synthesize and respond to transforming growth factor \u03b2 (TGF\u03b2) in vitro.","volume":"105","author":"Robey","year":"1988","journal-title":"J Cell Biol"},{"key":"2024013114210053400_bib24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/346281a0","article-title":"Negative regulation of transforming growth factor \u03b2 by the proteoglycan decorin.","volume":"346","author":"Yamaguchi","year":"1990","journal-title":"Nature"},{"key":"2024013114210053400_bib25","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1096\/fasebj.7.9.8330681","article-title":"Multi-faceted regulation of cell differentiation by extracellular matrix.","volume":"7","author":"Lin","year":"1993","journal-title":"FASEB J"},{"key":"2024013114210053400_bib26","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0022-5193(82)90388-5","article-title":"How does the extracellular matrix direct gene expression?","volume":"99","author":"Bissell","year":"1982","journal-title":"J Theor Biol"},{"key":"2024013114210053400_bib27","doi-asserted-by":"crossref","first-page":"13029","DOI":"10.1016\/S0021-9258(17)38833-6","article-title":"Coordinate regulation of collagen and alkaline phosphatase levels in chick embryo chondrocytes.","volume":"260","author":"Habuchi","year":"1985","journal-title":"J Biol Chem"},{"key":"2024013114210053400_bib28","first-page":"23","volume-title":"Essays in Biochemistry","author":"Ballard","year":"1977"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650101113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/10\/11\/1705\/56497494\/5650101113.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/10\/11\/1705\/56497494\/5650101113.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T15:01:18Z","timestamp":1710342078000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/10\/11\/1705-1712\/7500858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":28,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1995,11,1]]},"published-print":{"date-parts":[[1995,11,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650101113","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1995,11,1]]},"published":{"date-parts":[[1995,11,1]]}}}