{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:45:28Z","timestamp":1787064328148,"version":"build-2736575974"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[1995,11,1]],"date-time":"1995-11-01T00:00:00Z","timestamp":815184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA24607"],"award-info":[{"award-number":["CA24607"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI29796"],"award-info":[{"award-number":["AI29796"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bisphosphonates are potent inhibitors of bone resorption in vivo and are emerging as important and widely used drugs for the treatment of a variety of abnormal bone resorptive processes. In the current study we investigated the in vitro effects of 4-amino-1-hydroxybutylidene-1,1-bisphosphonate (alendronate), a recently developed, extremely potent bisphosphonate, on the immune functions of human peripheral blood mononuclear cells (PBMCs). PBMC proliferation induced by lectins, alloantigens, and a nominal antigen (tetanus toxoid) was inhibited in a dose-dependent manner by alendronate. Pretreatment of monocytes, but not T cells, with the compound at concentrations ranging from 10\u22124 to 10\u22128 M was inhibitory, indicating that alendronate acts selectively on antigen-presenting cells (APCs). Alendronate did not affect the viability of monocytes or T cells or the expression of cell surface molecules known to play critical roles in antigen presentation. Alendronate exhibited dose-dependent inhibition of the production of interleukin-1\u03b2 (IL-1\u03b2), interleukin-6 (IL-6), and tumor necrosis factor-\u03b1 (TNF-\u03b1) by activated monocytes. The inhibitory effect of 10\u22126 M alendronate on PBMC proliferation was reversed by 10 U\/ml recombinant rIL-1\u03b2, whereas other cytokines such as IL-6, TNF-\u03b1, and granulocyte-macrophage colony-stimulating factor (GM-CSF) had no effect. Thus, alendronate acts on monocytes to inhibit their antigen-presenting\/accessory cell functions through a mechanism that can be overcome by exogenous IL-1. The inhibitory effect of this agent on cytokine production may contribute to its inhibitory effect on bone resorption.<\/jats:p>","DOI":"10.1002\/jbmr.5650101115","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T11:02:12Z","timestamp":1265194932000},"page":"1719-1725","source":"Crossref","is-referenced-by-count":79,"title":["Inhibition of antigen-presenting cell function by alendronate in vitro"],"prefix":"10.1093","volume":"10","author":[{"given":"Paolo","family":"Sansoni","sequence":"first","affiliation":[{"name":"Clinica Medica Generale, University of Parma, Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Passeri","sequence":"additional","affiliation":[{"name":"Clinica Medica Generale, University of Parma, Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Fagnoni","sequence":"additional","affiliation":[{"name":"Clinica Medica Generale, University of Parma, Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahid","family":"Mohagheghpour","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Snelli","sequence":"additional","affiliation":[{"name":"Clinica Medica Generale, University of Parma, Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Brianti","sequence":"additional","affiliation":[{"name":"Clinica Medica Generale, University of Parma, Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar G.","family":"Dr. Engleman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California, 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