{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:18:23Z","timestamp":1777087103188,"version":"3.51.4"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1996,3,1]],"date-time":"1996-03-01T00:00:00Z","timestamp":825638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Hoffmann Laroche Laboratories"},{"name":"Department of radioimmunologie, Lariboisiere Hospital, Paris"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Craniosynostosis is a heterogeneous disorder characterized by premature fusion of the skull bone sutures. To evaluate the pathogenesis of premature cranial suture ossification in craniosynostosis, we have evaluated the histologic indices of bone formation and the characteristics of osteoblastic cells derived from normal and affected cranial sutures in 47 infants and children, aged 3\u201318 months, with nonsyndromic craniosynostosis. The histomorphometric analysis of normal and fused sutures showed an age-related decline in the extent of endosteal bone surface covered with osteoid and osteoblasts during postnatal suture ossification. Bone formation was 20\u201350% higher at 3\u20136 months of age in fused sutures compared with normal sutures in the same patients. Cells derived from normal and fused sutures displayed characteristics of the osteoblast phenotype in culture. Analysis of [3H]thymidine incorporation into DNA from 1\u201314 days of culture showed an age-related decrease in osteoblastic cell growth in both normal and affected sutures. The proliferation of osteoblastic cells isolated from fused sutures was similar at all ages to that of cells isolated from normal sutures in the same patients. In contrast, alkaline phosphatase activity and osteocalcin production by osteoblastic cells cultured in basal conditions and after stimulation with 1,25-dihydroxyvitamin D (1,25[OH]2D3), were 53\u201374% higher in fused sutures compared with cells isolated from normal sutures in the same patients. The results indicate that bone formation activity at the suture site is locally increased in craniosynostosis, and this disorder is associated with increased in vitro parameters of osteoblastic cell differentiation, suggesting that an increased maturation of osteoblastic cells at the site of the suture leads to the premature ossification in nonsyndromic craniosynostosis.<\/jats:p>","DOI":"10.1002\/jbmr.5650110314","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T15:31:40Z","timestamp":1265211100000},"page":"401-407","source":"Crossref","is-referenced-by-count":76,"title":["Increased bone formation and osteoblastic cell phenotype in premature cranial suture ossification (craniosynostosis)"],"prefix":"10.1093","volume":"11","author":[{"given":"Cinderella","family":"de Pollack","sequence":"first","affiliation":[{"name":"INSERM U349, Cell and Molecular Biology of Bone and Cartilage, Lariboisi\u00e8re Hospital, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Renier","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurosurgery, Hopital Necker Enfants-Malades, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Hott","sequence":"additional","affiliation":[{"name":"INSERM U349, Cell and Molecular Biology of Bone and Cartilage, Lariboisi\u00e8re Hospital, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre J.","family":"Dr. Marie","sequence":"additional","affiliation":[{"name":"INSERM U349, Cell and Molecular Biology of Bone and Cartilage, Lariboisi\u00e8re Hospital, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1996,3,1]]},"reference":[{"key":"2024013114254828100_bib1","volume-title":"Craniofacial Surgery for Craniosynostosis","author":"Marchac","year":"1982"},{"key":"2024013114254828100_bib2","volume-title":"Craniosynostosis: Diagnosis, Evaluation and Management","author":"Cohen","year":"1986"},{"key":"2024013114254828100_bib3","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1002\/ajmg.1320550422","article-title":"A genetic study of non syndromic coronal craniosynostosis","volume":"55","author":"Lajeunie","year":"1995","journal-title":"Am J Med Genet"},{"key":"2024013114254828100_bib4","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1002\/ajmg.1320470507","article-title":"Sutural biology and the correlates of craniosynostosis","volume":"47","author":"Cohen","year":"1993","journal-title":"Am J Med Gen"},{"key":"2024013114254828100_bib5","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/0092-8674(93)90379-5","article-title":"A mutation in the homeodomain of the human MSX2 gene in a family affected with autosomal dominant craniosynostosis","volume":"75","author":"Jabs","year":"1993","journal-title":"Cell"},{"key":"2024013114254828100_bib6","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/ng0994-98","article-title":"Mutations in the fibroblast growth factor receptor 2 gene cause Crouzon syndrome","volume":"8","author":"Reardon","year":"1994","journal-title":"Nat Genet"},{"key":"2024013114254828100_bib7","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/ng0295-173","article-title":"Identical mutations in the FGFR2 gene cause both Pfeiffer and Crouzon syndromes phenotypes","volume":"9","author":"Rutland","year":"1995","journal-title":"Nat Genet"},{"key":"2024013114254828100_bib8","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/ng0295-108","article-title":"FGFR2 mutations in Pfeiffer syndrome","volume":"9","author":"Lajeunie","year":"1995","journal-title":"Nat Genet"},{"key":"2024013114254828100_bib9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/ng0295-165","article-title":"Apert syndrome results from localized mutations of FGFR2 and is allelic with Crouzon syndrome","volume":"9","author":"Wilkie","year":"1995","journal-title":"Nat Genet"},{"issue":"12","key":"2024013114254828100_bib10","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1002\/jbmr.5650091202","article-title":"Human osteoblastic cells: A potential tool to assess the etiology of pathologic bone formation","volume":"9","author":"Marie","year":"1994","journal-title":"J Bone Miner Res"},{"key":"2024013114254828100_bib11","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/BF03354642","article-title":"Human osteoblastic cells: Relationship with bone formation","volume":"56S","author":"Marie","year":"1995","journal-title":"Calcif Tissue Int"},{"issue":"1","key":"2024013114254828100_bib12","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3109\/10520298709107965","article-title":"Glycol methacrylate as an embedding medium for bone tissue","volume":"62","author":"Hott","year":"1987","journal-title":"Stain Tech"},{"key":"2024013114254828100_bib13","first-page":"13","volume-title":"Bone Histomorphometry","author":"Baron","year":"1983"},{"key":"2024013114254828100_bib14","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/jbmr.5650020617","article-title":"Bone histomorphometry: standardization of nomenclature, symbols and units","volume":"2","author":"Parfitt","year":"1987","journal-title":"J Bone Miner Res"},{"key":"2024013114254828100_bib15","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/BF02624601","article-title":"Culture and behavior of osteoblastic cells isolated from normal trabecular bone surfaces","volume":"25","author":"Marie","year":"1989","journal-title":"In Vitro Cell Biol Dev"},{"key":"2024013114254828100_bib16","first-page":"541","article-title":"Osteogenesis induced by osteoblastic cells derived from newborn human calvaria: A new in vitro model of human osteoblast differentiation","volume":"52","author":"de Pollak","year":"1993","journal-title":"Calcif Tissue Int"},{"key":"2024013114254828100_bib17","first-page":"244","volume-title":"Expression of the osteoblastic phenotype","author":"Rodan","year":"1990"},{"key":"2024013114254828100_bib18","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1172\/JCI115418","article-title":"Decreased DNA synthesis by cultured osteoblastic cells in eugonadal osteoporotic men with defective bone formation","volume":"88","author":"Marie","year":"1991","journal-title":"J Clin Invest"},{"key":"2024013114254828100_bib19","first-page":"73","article-title":"The structure and development of cranial and facial sutures","volume":"90","author":"Pritchard","year":"1956","journal-title":"J Anat"},{"key":"2024013114254828100_bib20","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1159\/000144486","article-title":"Embryonic development of the coronal suture in man and rat","volume":"93","author":"Markens","year":"1975","journal-title":"Acta Anat (Basel)"},{"key":"2024013114254828100_bib21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1002\/jcp.1041510202","article-title":"Age-related changes in hyaluronan, proteoglycan, collagen, and osteonectin synthesis by human bone cells","volume":"151","author":"Fedarko","year":"1992","journal-title":"J Cell Physiol"},{"key":"2024013114254828100_bib22","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1096\/fasebj.4.13.2210157","article-title":"Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation","volume":"4","author":"Stein","year":"1990","journal-title":"FASEB J"},{"key":"2024013114254828100_bib23","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1177\/40.9.1506671","article-title":"Isolation of monoclonal antibodies recognizing rat bone-associated molecules in vitro and in vivo","volume":"40","author":"Turksen","year":"1992","journal-title":"J Histochem Cytochem"},{"key":"2024013114254828100_bib24","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/S0006-291X(87)80250-4","article-title":"Tissue specificity and developmental expression of rat osteopontin","volume":"148","author":"Yoon","year":"1987","journal-title":"Biochem Biophys Res Commun"},{"key":"2024013114254828100_bib25","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1002\/jcb.240570108","article-title":"Temporal variation of c-fos proto-oncogene expression during osteoblast differentiation and osteogenesis in developing bone","volume":"57","author":"Machwate","year":"1995","journal-title":"J Cell Biochem"},{"key":"2024013114254828100_bib26","first-page":"313","article-title":"Sutural closure in rabbit and man: A morphological and histochemical study","volume":"125","author":"Persson","year":"1978","journal-title":"J Anat"},{"key":"2024013114254828100_bib27","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3171\/jns.1981.54.3.0384","article-title":"Suture pathology in craniosynostosis","volume":"54","author":"Albright","year":"1981","journal-title":"J Neurosurg"},{"key":"2024013114254828100_bib28","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1432-1033.1993.tb17660.x","article-title":"Regulation of vertebrate homeobox-containing genes by morphogens","volume":"212","author":"Mavilio","year":"1993","journal-title":"Eur J Biochem"},{"key":"2024013114254828100_bib29","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1172\/JCI113318","article-title":"Growth factors and the regulation of bone remodeling","volume":"81","author":"Canalis","year":"1988","journal-title":"J Clin Invest"},{"key":"2024013114254828100_bib30","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1038\/ng0494-348","article-title":"Msxl deficient mice exhibit cleft palate and abnormalities of craniofacial and tooth development","volume":"6","author":"Satokata","year":"1994","journal-title":"Nat Genet"},{"key":"2024013114254828100_bib31","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0167-4781(93)90086-S","article-title":"Expression of a human homeobox-containing gene is regulated bby 1,25(OH)2 D3 in bone cells","volume":"1174","author":"Hodgkinson","year":"1993","journal-title":"Biochim Biophys Acta"},{"key":"2024013114254828100_bib32","first-page":"1484","article-title":"Msx-2\/Hox 8.1: A transcriptional regulator of the rat osteocalcin promoter","volume":"8","author":"Towler","year":"1994","journal-title":"Mol Endocrinol"}],"container-title":["Journal of Bone and Mineral Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbmr.5650110314","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/11\/3\/401\/56503379\/5650110314.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jbmr\/article-pdf\/11\/3\/401\/56503379\/5650110314.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T15:02:00Z","timestamp":1710342120000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbmr\/article\/11\/3\/401-407\/7501256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3,1]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1996,3,1]]},"published-print":{"date-parts":[[1996,3,1]]}},"URL":"https:\/\/doi.org\/10.1002\/jbmr.5650110314","relation":{},"ISSN":["0884-0431","1523-4681"],"issn-type":[{"value":"0884-0431","type":"print"},{"value":"1523-4681","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1996,3,1]]},"published":{"date-parts":[[1996,3,1]]}}}