{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:05:07Z","timestamp":1762099507297},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,3,27]],"date-time":"2001-03-27T00:00:00Z","timestamp":985651200000},"content-version":"vor","delay-in-days":85,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mouse <jats:italic>Cyp2b\u201010<\/jats:italic> gene is inducible by treatment with estradiol as well as so\u2010called phenobarbital (PB)\u2010like inducers. To identify 5\u2032\u2010flanking elements responsible for induction by estradiol, we carried out reporter gene assays using a primary mouse hepatocyte culture system. <jats:italic>Cyp2b\u201010<\/jats:italic> gene\u2010driven luciferase activities were induced by estradiol as well as PB in this system. Deletion analysis demonstrated that the sequence contained within the region from \u22122331 bp to \u22122281 bp was responsible for the estradiol\u2010induced luciferase activity. This region corresponds to the core element of PB\u2010responsive enhancer module (PBREM). Several nucleotide mutations in the putative binding sites of the PBREM core element showed that the NR1 site was required for estradiol induction, and the same element was required for PB induction. These results indicate that estradiol induces <jats:italic>Cyp2b\u201010<\/jats:italic> gene expression via PBREM. \u00a9 2001 John Wiley &amp; Sons, Inc. 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