{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:31:15Z","timestamp":1791307875181,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T00:00:00Z","timestamp":1225152000000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Black widow spider is one of the most poisonous spiders in the world. Up to now, there have been few systematic analyses of the spider venom components, and the mechanism of action of the venom has not been completely understood. In this work, we employed combinative proteomic strategy to analyze the venom collected from living adult spider <jats:italic>Latrodectus tredecimguttatus<\/jats:italic> by electrical stimulation. The experiments demonstrated that the venom is primarily composed of high molecular weight proteins and has high abundance proteins around 100 kDa. The content of peptides and proteins with low molecular weight is low. A total of 75 nonredundant venom proteins with distinct function were unambiguously identified. Besides the known black widow spider venom proteins including latrotoxins, a variety of hydrolases and other proteins with special activity were found in the venom, such as proteinase, phospholipase, phosphatase, nuclease, fucolectin, venom allergen antigen 5\u2010like protein and trypsin inhibitor, and so on. Their possible biological actions and relationship with latrodectism were discussed. The results help to understand the complexity and action mechanism of <jats:italic>L. tredecimguttatus<\/jats:italic> venom. \u00a9 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:328\u2013336, 2008; Published online in Wiley InterScience (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.interscience.wiley.com\">www.interscience.wiley.com<\/jats:ext-link>). DOI 10.1002\/jbt.20244<\/jats:p>","DOI":"10.1002\/jbt.20244","type":"journal-article","created":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T22:34:27Z","timestamp":1225233267000},"page":"328-336","source":"Crossref","is-referenced-by-count":30,"title":["Proteomic analysis of <i>Latrodectus tredecimguttatus<\/i> venom for uncovering potential latrodectism\u2010related proteins"],"prefix":"10.1002","volume":"22","author":[{"given":"Zhigui","family":"Duan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianchun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songping","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10,28]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Advances in spider taxonomy 1988\u20131991: with synonymies and transfers 1940\u20131980","author":"Platnick NI","year":"1993"},{"key":"e_1_2_1_3_2","first-page":"1","volume-title":"Advances in spider taxonomy 1992\u20131995: with redescriptions 1940\u20131980","author":"Platnick NI","year":"1997"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1580\/1080-6032(1997)008[0226:WSELAW]2.3.CO;2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(76)90077-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(72)90357-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb04248.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-0361-9_16"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(72)90115-8"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.68.3.462"},{"key":"e_1_2_1_11_2","first-page":"1138","article-title":"Isolation and properties of insect\u2010specific neurotoxins from venoms of the spider Lactodectus mactans tredecimguttatus","volume":"16","author":"Krasnoperov VG","year":"1990","journal-title":"Bioorg Khim"},{"key":"e_1_2_1_12_2","first-page":"1567","article-title":"A crustacean\u2010specific neurotoxin from the venom of the black widow spider Latrodectus mactans tredecimguttatus","volume":"16","author":"Krasnoperov VG","year":"1990","journal-title":"Bioorg Khim"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.87.1.297"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.88.1.59"},{"key":"e_1_2_1_15_2","first-page":"537","article-title":"Cloning and structure of gene encoded alpha\u2010latrocrustoxin from the black widow spider venom","volume":"2","author":"Danilevich VN","year":"1999","journal-title":"Bioorg Khim"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.13.7535"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)81250-R"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb17741.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)00166-6"},{"key":"e_1_2_1_20_2","first-page":"469","article-title":"Isolation and characterization of biological properties of inhibitors angiotensin\u20101\u2010converting enzyme from the spider venom Latrodectus tredecimguttatus","volume":"38","author":"Akhunov AA","year":"1992","journal-title":"Agents Actions Suppl"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0162-3109(95)00081-X"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655401300077423"},{"key":"e_1_2_1_23_2","first-page":"437","article-title":"Toxic pictures produced spiders bites in Chile: Latrodectism and loxoscelism","volume":"131","author":"Schenone H","year":"2003","journal-title":"Rev Med Chil"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.73.2.245"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.2.991"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01512"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03165785"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305552"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199210000-00008"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00199-4"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(97)00083-4"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(99)00197-X"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(01)00254-7"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2001000700002"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.07.012"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215540405200404"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200402024"},{"key":"e_1_2_1_39_2","first-page":"1","volume-title":"Animal toxins","author":"Smith DS","year":"1966"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0700192"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600785"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.20244","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.20244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T13:06:12Z","timestamp":1699967172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.20244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1002\/jbt.20244"],"URL":"https:\/\/doi.org\/10.1002\/jbt.20244","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9]]}}}