{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:43:21Z","timestamp":1774547001528,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,11,15]],"date-time":"2010-11-15T00:00:00Z","timestamp":1289779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp;amp; Molecular Tox"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2\u2010acetylpyridine and malonic acid dihydrazide, <jats:italic>N<\/jats:italic>\u2032,<jats:italic>N<\/jats:italic>\u2032<jats:sup>2<\/jats:sup>\u2010bis[(1<jats:italic>E<\/jats:italic>)\u20101\u2010(2\u2010pyridyl)ethylidene]propanedihydrazide (Cd(II)H<jats:sub>2<\/jats:sub>L), with calf thymus DNA (CT\u2010DNA) was monitored by blue shift in UV\u2013vis spectra of the complex. The binding constant of Cd(II)H<jats:sub>2<\/jats:sub>L complex with CT\u2010DNA was determined (<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub> = 1.8 \u00d7 10<jats:sup>4<\/jats:sup> M<jats:sup>\u22121<\/jats:sup>) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II)H<jats:sub>2<\/jats:sub>L complex. The Cd(II)H<jats:sub>2<\/jats:sub>L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub\u2010G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl\u20102 protein with the Cd(II)H<jats:sub>2<\/jats:sub>L complex. \u00a9 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:175\u2013182 2011; View this article online at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"wileyonlinelibrary.com\">wileyonlinelibrary.com<\/jats:ext-link>. DOI 10.1002\/jbt.20374<\/jats:p>","DOI":"10.1002\/jbt.20374","type":"journal-article","created":{"date-parts":[[2010,11,16]],"date-time":"2010-11-16T04:40:49Z","timestamp":1289882449000},"page":"175-182","source":"Crossref","is-referenced-by-count":17,"title":["A comparative study of DNA binding and cell cycle phase perturbation by the dinuclear complex of Cd(II) with the condensation product of 2\u2010acetylpyridine and malonic acid dihydrazide <i>N<\/i>\u2032,<i>N<\/i>\u2032<sup>2<\/sup>\u2010bis[(1<i>E<\/i>)\u20101\u2010(2\u2010pyridyl)ethylidene]propanedihydrazide"],"prefix":"10.1002","volume":"25","author":[{"given":"Miroslava","family":"Vuj\u010di\u0107","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Lazi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milica","family":"Milenkovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Du\u0161an","family":"Sladi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sini\u0161a","family":"Radulovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nenad","family":"Filipovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"An\u0111elkovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja981937r"},{"key":"e_1_2_5_3_2","first-page":"288","volume-title":"Bioorganic chemistry: Nucleic acids","author":"Mountzouris JA","year":"1996"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(03)00081-4"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11243-004-3173-1"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/MSF.518.513"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2007.10.013"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1974.227.5.1033"},{"key":"e_1_2_5_9_2","unstructured":"IARC.Cadmium and cadmium compounds. In IARC Monographs on evaluation of carcinogenic risks to humans. Beryllium cadmium and mercury exposures in the glass manufacturing industry Vol. 58: International agency for research on cancer Lyion France 1993. pp119\u2013237."},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(02)00412-9"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1439-7633(20010202)2:2<119::AID-CBIC119>3.0.CO;2-5"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-005-0045-x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01640a067"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00394a013"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.13.6127"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_2_5_17_2","first-page":"119","volume-title":"Flow cytometry, a practical approach","author":"Ormerod MG","year":"1994"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(97)00150-4"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ic061518s"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2308"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002800050767"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00294a054"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2707"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2004.12.018"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20248"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061029098"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.0211037"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.poly.2009.05.002"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009232311677"}],"container-title":["Journal of Biochemical and Molecular Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbt.20374","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbt.20374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T09:51:41Z","timestamp":1726739501000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbt.20374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,15]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1002\/jbt.20374"],"URL":"https:\/\/doi.org\/10.1002\/jbt.20374","archive":["Portico"],"relation":{},"ISSN":["1095-6670","1099-0461"],"issn-type":[{"value":"1095-6670","type":"print"},{"value":"1099-0461","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,15]]}}}