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To understand these mechanisms, we measured acid\u2010induced mucin secretion in human bronchial epithelial cells. In the present study, acid induced inward currents of transient receptor potential vanilloid (TRPV)1 and mucin 5AC (MUC5AC) secretion dose dependently, which were inhibited by TRPV1 antagonist capsazepine in a concentration\u2010dependent manner. TRPV1 agonist capsaicin mediated a concentration\u2010dependent increase in TRPV1 inward currents and MUC5AC secretion. Furthermore, capsaicin enhanced acid\u2010induced TRPV1 inward currents and MUC5AC secretion. Acid\u2010induced Ca<jats:sup>2+<\/jats:sup>influx was prevented by capsazepine dose dependently and enhanced by capsaicin. Pretreatment only with capsaicin also increased the Ca<jats:sup>2+<\/jats:sup>concentration in a concentration\u2010dependent manner. These data suggest that pharmacological inhibition of calcium\u2010permeable TRPV1 receptors could be used to prevent acid\u2010induced mucin secretion, thereby providing a potential mechanism to reduce their toxicity. \u00a9 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:179\u2013186, 2012; View this article online at<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"wileyonlinelibrary.com\">wileyonlinelibrary.com<\/jats:ext-link>. 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