{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:03:41Z","timestamp":1773659021737,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,4,29]],"date-time":"2013-04-29T00:00:00Z","timestamp":1367193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Comprehensive knowledge of venom composition is very important for effective management of snake envenomation and antivenom preparation. <jats:italic>Daboia russelii<\/jats:italic> venom from the eastern region of India is the most neurotoxic among the four venom samples investigated. From the eastern <jats:italic>D. russelii<\/jats:italic> venom sample, neurotoxic peptide has been purified by combined method of ion exchange gel permeation chromatography and reversed phase high performance liquid chromatography. Molecular weight of <jats:italic>Daboia<\/jats:italic> neurotoxin III (DNTx\u2010III) found to be 6,849 Da (as measured on matrix\u2010assisted laser desorption\/ionisation\u2010time of flight mass spectrometer), and N\u2010terminal amino acid sequences is I K C F I T P D U T S Q A. Approximate LD<jats:sub>50<\/jats:sub> dosage was 0.24 mg\/kg body weight. It produced concentration\u2010 and time\u2010dependent inhibition of indirectly stimulated twitches of <jats:italic>Rana hexadactyla<\/jats:italic> sciatic nerve gastrocnemius muscle preparations. Chemical modification of DNTx\u2010III tryptophan residue(s) reduced the twitch height inhibition property of toxin, signifying the importance of tryptophan residues for the neurotoxic function. This type of neurotoxic peptide is unique to east Indian regional <jats:italic>D. russelii<\/jats:italic> venom. \u00a9 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:295\u2010304, 2013; View this article online at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.wileyonlinelibrary.com\">wileyonlinelibrary.com<\/jats:ext-link>. 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