{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:40:16Z","timestamp":1762101616355},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,10,26]],"date-time":"2014-10-26T00:00:00Z","timestamp":1414281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Agri Ibrahim Cecen Unicersity Scientific Research Projects Unit (BAP)","award":["BAP2012\/MYO-02"],"award-info":[{"award-number":["BAP2012\/MYO-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Carbonic anhydrase (CA) was purified from A\u011fr\u0131 Bal\u0131k Lake trout gill (fCA) by affinity chromatography on a sepharose 4B\u2010tyrosine\u2010sulfanilamide column. The fCA enzyme was purified with about a 303.9 purification factor, a specific activity 4130.4 EU (mg\u2010protein)<jats:sup>\u20131<\/jats:sup>, and a yield of 79.3 by using sepharose\u20104B\u2010<jats:sc>l<\/jats:sc> tyrosine\u2010sulfanilamide affinity gel chromatography. The molecular weight determined by sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis (SDS\u2013PAGE) was found to be about 29.9 kDa. The kinetic parameters, <jats:italic>K<\/jats:italic><jats:sub>M<\/jats:sub> and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> were determined for the 4\u2010nitrophenyl acetate hydrolysis reaction. Some sulfonamides were tested as inhibitors against the purified CA enzymes. The <jats:italic>K<jats:sub>i<\/jats:sub><\/jats:italic> constants for mafenide (<jats:bold>1<\/jats:bold>), <jats:italic>p<\/jats:italic>\u2010toluenesulfonamide (<jats:bold>2<\/jats:bold>), 2\u2010bromo\u2010benzene sulfonamide (<jats:bold>3<\/jats:bold>), 4\u2010chlorobenzene sulfonamide (<jats:bold>4<\/jats:bold>), 4\u2010amino\u20106\u2010chloro\u20101\u20133 benzenedisulfonamide (<jats:bold>5<\/jats:bold>), sulfamethazine (<jats:bold>6<\/jats:bold>), sulfaguanidine (<jats:bold>7<\/jats:bold>), sulfadiazine (<jats:bold>8<\/jats:bold>), and acetozazolamide (<jats:bold>9<\/jats:bold>) were in the range of 7.5\u2013108.75 \u03bcM.<\/jats:p>","DOI":"10.1002\/jbt.21675","type":"journal-article","created":{"date-parts":[[2014,10,26]],"date-time":"2014-10-26T06:03:31Z","timestamp":1414303411000},"page":"123-128","source":"Crossref","is-referenced-by-count":8,"title":["Purification and Characterization of Carbonic Anhydrase from A\u011fr\u0131 Bal\u0131k Lake Trout Gill (<i>Salmo trutta labrax<\/i>) and Effects of Sulfonamides on Enzyme Activity"],"prefix":"10.1002","volume":"29","author":[{"given":"Ramazan","family":"Demirdag","sequence":"first","affiliation":[{"name":"School of Health Agri Ibrahim Cecen University  Agri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veysal","family":"Comakli","sequence":"additional","affiliation":[{"name":"School of Health Agri Ibrahim Cecen University  Agri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muslum","family":"Kuzu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Agri Ibrahim Cecen University  Agri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emrah","family":"Yerlikaya","sequence":"additional","affiliation":[{"name":"School of Health Siirt University  Siirt Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"\u015eent\u00fcrk","sequence":"additional","affiliation":[{"name":"Art and Science Faculty Agri Ibrahim Cecen University Chemistry Department  Agri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,26]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"61","article-title":"Carbonic anhydrase inhibitors","volume":"1","author":"Supuran CT","year":"2001","journal-title":"Curr Med Chem"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780203475300.ch1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051102"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360601176048"},{"key":"e_1_2_6_6_1","unstructured":"G\u00f6l\u00fcB 2014. 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