{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:23:31Z","timestamp":1777501411468,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T00:00:00Z","timestamp":1418169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The present study is designed to investigate the isolation and characterization of biological and biochemical active venom protein from sea snake, <jats:italic>Enhydrina schistosa<\/jats:italic>. The highest purification peaks in ion\u2010exchange chromatography on DEAE\u2010cellulose column were obtained for fraction numbers 39\u201349 when eluted with 0.35\u20130.45 M NaCl. Eighty per cent purity was obtained in the final stage of purification, and a single protein band of about 44 kDa was visualized in SDS\u2010polyacrylamide gel under reducing condition. Purified venom protein expressed as haemolytic, cytotoxicity and proteolytic activities with lethal concentration (LC<jats:sub>50<\/jats:sub>) at 2.0 \u03bcg\/mL. Venom protein exhibits enzymatic activity and hydrolyzed casein and gelatin. Gelatinolytic activity was optimal at pH 5\u20139. In conclusion, the present results suggested that the sea snake venom might be feasible sources for biologically active substances. Thus, this low molecular weight component of the venom protein could be used in potentially serve biological and pharmaceutical aspects.<\/jats:p>","DOI":"10.1002\/jbt.21678","type":"journal-article","created":{"date-parts":[[2014,12,10]],"date-time":"2014-12-10T13:24:01Z","timestamp":1418217841000},"page":"140-147","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and Characterization of Biologically Active Venom Protein from Sea Snake <i>Enhydrina schistosa<\/i>"],"prefix":"10.1002","volume":"29","author":[{"given":"Palani","family":"Damotharan","sequence":"first","affiliation":[{"name":"Centre of Advanced Study in Marine Biology Faculty of Marine Sciences Annamalai University  Parangipettai 608 502 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anguchamy","family":"Veeruraj","sequence":"additional","affiliation":[{"name":"Centre of Advanced Study in Marine Biology Faculty of Marine Sciences Annamalai University  Parangipettai 608 502 India"},{"name":"Centre for Ocean Research (SU\u2010NIOT Joint Initiative Research Centre) Rajiv Gandhi Salai, Sathyabama University, Chennai 600 119, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muthuvel","family":"Arumugam","sequence":"additional","affiliation":[{"name":"Centre of Advanced Study in Marine Biology Faculty of Marine Sciences Annamalai University  Parangipettai 608 502 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thangavel","family":"Balasubramanian","sequence":"additional","affiliation":[{"name":"Centre of Advanced Study in Marine Biology Faculty of Marine Sciences Annamalai University  Parangipettai 608 502 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00268-X"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(91)90116-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2335845100"},{"key":"e_1_2_7_5_1","volume-title":"Poisonous and venomous marine animals of the world","author":"Halsteatd BW","year":"1988"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0160-9327(93)90104-B"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)60072-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1197"},{"key":"e_1_2_7_9_1","first-page":"29","volume-title":"Snakes of the Darwin Area","author":"Gow GF","year":"1977"},{"key":"e_1_2_7_10_1","first-page":"235","volume-title":"Poisonous and venomous marine animals of the world","author":"Tu AT","year":"1988"},{"key":"e_1_2_7_11_1","first-page":"380","volume-title":"Marine toxins origin structure and molecular pharmacology","author":"Tu AT","year":"1990"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1989.tb06466.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.42.070173.001315"},{"key":"e_1_2_7_14_1","first-page":"151","volume-title":"Venoms. chemistry and molecular biology","author":"Tu AT","year":"1977"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.01.008"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_7_18_1","first-page":"333","volume-title":"Probit analysis","author":"Finney DJ","year":"1971"},{"key":"e_1_2_7_19_1","first-page":"1","volume-title":"Micro hemolytic assay: training manual on advanced techniques in marine biotechnology. 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