{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:09:50Z","timestamp":1758636590896},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hubei 2011 Cooperative Innovation Center (grant 4)","award":["2011JH-2014CXTT04"],"award-info":[{"award-number":["2011JH-2014CXTT04"]}]},{"name":"Hubei Provincial Department of Education Research Project","award":["Q20142102"],"award-info":[{"award-number":["Q20142102"]}]},{"name":"New Century Excellent Talents in Wuhan University by the Ministry of Education of China","award":["NCET-10-0651"],"award-info":[{"award-number":["NCET-10-0651"]}]},{"name":"Wuhan City Science and Technology Foundation of China","award":["2013070204020046"],"award-info":[{"award-number":["2013070204020046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Two native peptides with disulfide\u2010directed hairpin (DDH) fold, LaIT1 and LITX, were recently isolated from scorpion venom, a development that offered insights into exploring the evolutionary linkage between DDH and inhibitor cystine knot (ICK) peptides. In this work, we isolated and identified the full\u2010length cDNAs of LaTI1, a representative member with DDH fold, and further determined its complete gene structure. The precursor organization of LaIT1 is similar to that of ICK peptides. The LaIT1 gene contains four exons interrupted by three unique introns and differed from ICK peptides, suggesting divergent genomic organizations of DDH peptides and ICK peptides. Phylogenetic analysis further showed that the \u201csimple\u201d DDH peptide originates from the \u201ccomplex\u201d ICK peptide, rather than the reverse. To the best of our knowledge, this is the first report on the genomic organization of DDH\u2010fold peptides, and it presents new evidence of an evolutionary linkage between ICK and DDH peptides.<\/jats:p>","DOI":"10.1002\/jbt.21686","type":"journal-article","created":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T07:09:18Z","timestamp":1428908958000},"page":"207-212","source":"Crossref","is-referenced-by-count":6,"title":["Cloning and Genomic Characterization of a Natural Insecticidal Peptide LaIT1 with Unique DDH Structural Fold"],"prefix":"10.1002","volume":"29","author":[{"given":"Jing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Hubei University of Medicine  Hubei People's Republic of China"},{"name":"State Key Laboratory of Virology College of Life Sciences Wuhan University  Hubei People's Republic of 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Sciences Wuhan University  Hubei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingliang","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology College of Life Sciences Wuhan University  Hubei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongyun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Hubei University of Medicine  Hubei People's Republic of China"},{"name":"State Key Laboratory of Virology College of Life Sciences Wuhan University  Hubei People's Republic of 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