{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:52:18Z","timestamp":1761519138381,"version":"3.37.3"},"reference-count":17,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T00:00:00Z","timestamp":1431302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004509","name":"Department of Health of Guangdong Province","doi-asserted-by":"publisher","award":["ZA20130006"],"award-info":[{"award-number":["ZA20130006"]}],"id":[{"id":"10.13039\/501100004509","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biochem &amp; Molecular Tox"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In this study, we purified and characterized the procoagulant protein FV\u20102 from <jats:italic>Daboia russelli siamensis<\/jats:italic> (Myanmar) venom using ion\u2010exchange chromatography on CM\u2010Sephadex C\u201050 and gel filtration on Superdex<jats:sup>TM<\/jats:sup> G\u201075 column. The activation of factor X and prothrombin was determined, respectively, by specific chromogenic substrates. The fibrinogen\u2010clotting activity, thermal stability, and pH stability were also determined. The N\u2010treminal sequence was determined by the National Center of Biomedical Analysis of China. In the end, FV\u20102 was achieved with a molecular weight of 13,608.0 Da. It could activate factor X, but did not affect prothrombin or fibrinogen. The suitable pH was 6.5\u20137.5, and the suitable temperature ranged from 25 to 60\u00b0C. The N\u2010terminal sequence was Asn\u2010Phe\u2010Phe\u2010Gln\u2010Phe\u2010Ala\u2010Glu\u2010Met\u2010Ile\u2010Val\u2010Lys\u2010Met\u2010Thr\u2010Gly\u2010Lys. Taken together, our studies suggest that FV\u20102 is a factor X\u2013activating enzyme, which can activate factor X to factor Xa, but it has no effect on prothrombin and fibrinogen.<\/jats:p>","DOI":"10.1002\/jbt.21713","type":"journal-article","created":{"date-parts":[[2015,5,11]],"date-time":"2015-05-11T09:17:38Z","timestamp":1431335858000},"page":"465-471","source":"Crossref","is-referenced-by-count":2,"title":["Purification, Partial Characterizations, and N\u2010Terminal Amino Acid Sequence of a Procoagulant Protein FV\u20102 from <i>Daboia Russelli Siamensis<\/i> (Myanmar) Venom"],"prefix":"10.1002","volume":"29","author":[{"given":"Weiwei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Regenerative Medicine Ministry of Education, Jinan University  Guangzhou 510632 People's Republic of China"},{"name":"Department of Developmental and Regenerative Biology Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanlin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunjian","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Medical College Jinan University  Guangzhou 510632 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Guangdong Institute for Food and Drug Control  Guangdong Province Guangzhou 510180 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700777"},{"key":"e_1_2_5_3_1","first-page":"76","article-title":"Coagulation studies on reptilase, an extract of the venom from Bothrops jararaca","volume":"1","author":"Blomback B","year":"1958","journal-title":"Thromb Diath Haemorr"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/13423"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.12.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2007.04.012"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2011.05.014"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0840052"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"10644","DOI":"10.1016\/S0021-9258(17)34108-X","article-title":"Factor X\u2010activating glycoprotein of Russell's viper venom. 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