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We have purified a 7579.5 \u00b1 0.591 Da 3FTx named as Nk\u20103FTx from the venom of <jats:italic>Naja kaouthia<\/jats:italic> of North East India origin. The primary structure was determined by a combination of N\u2010terminal sequencing and electrospray ionization \u00a0liquid chromatography\u2010mass spectrometry\/mass spectrometry. Biochemical and biological characterization reveal that it is nontoxic to human cell lines and exhibit mild anticoagulant activity when tested on citrated human plasma. Nk\u20103FTx was found to affect the compound action potential (CAP) and nerve conduction velocity of isolated toad sciatic nerve. This is the first report of a non\u2010conventional 3FTx from <jats:italic>Naja kaouthia<\/jats:italic> venom that reduces CAP for its neurotoxic effect. Further studies can be carried out to understand the mechanism of action and to explore its potential therapeutic application.<\/jats:p>","DOI":"10.1002\/jbt.21734","type":"journal-article","created":{"date-parts":[[2015,8,21]],"date-time":"2015-08-21T07:53:02Z","timestamp":1440143582000},"page":"59-70","source":"Crossref","is-referenced-by-count":18,"title":["Purification and Characterization of Nk\u20103FTx: A Three Finger Toxin from the Venom of North East Indian Monocled Cobra"],"prefix":"10.1002","volume":"30","author":[{"given":"Diganta","family":"Das","sequence":"first","affiliation":[{"name":"Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology Tezpur University  Tezpur\u2010 784028 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maitreyee","family":"Sharma","sequence":"additional","affiliation":[{"name":"Molecular Toxinology Laboratory, Department of Molecular Biology and Biotechnology Tezpur University  Tezpur\u2010 784028 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