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In this study, the role of lipid rafts in ochratoxin A\u2010induced toxicity was investigated using Hepatoblastoma Cell Line HepG\u20102 cells. Disruption of cholesterol\u2010containing lipid rafts enhanced Ochratoxin A (OTA) toxicity, as shown by increased lactate dehydrogenase leakage, increased reactive oxygen species level and reduction of superoxide dismutase activity in a time\u2010dependent manner. Isobaric tags for relative and absolute quantitation\u2010based proteomics of the cell membranes showed that nearly 85.5% proteins were downregulated by OTA, indicating that OTA inhibited the membrane protein synthesis. Most of altered proteins were involved in Gene Ontology \u201ctransport\u201d, \u201ccell adhesion\u201d and \u201cvesicle\u2010mediated transport\u201d. In conclusion, lipid rafts play a key role in OTA\u2010induced cytotoxicity. This study provides insight into how OTA toxicity is regulated by the plasma membrane, especially the lipid rafts.<\/jats:p>","DOI":"10.1002\/jbt.21738","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T14:45:15Z","timestamp":1440513915000},"page":"71-79","source":"Crossref","is-referenced-by-count":14,"title":["Lipid Rafts Disruption Increases Ochratoxin A Cytotoxicity to Hepatocytes"],"prefix":"10.1002","volume":"30","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"},{"name":"Beijing Engineering and Technology Research Center of Food Additives Beijing Technology &amp; Business University (BTBU)  Beijing 100048 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhe","family":"Qi","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanjuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunbo","family":"Luo","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Science University of Maryland  College Park MD 20742 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junran","family":"Hao","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunlun","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wentao","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Food Safety, College of Food Science and Nutritional Engineering China 6 Agricultural University  Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"IARC. 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