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The purification of the enzymes was performed using 2',5'\u2010ADP sepharose 4B affinity material. The subunit and the natural molecular weights were analyzed by SDS\u2010PAGE and gel filtration. Biochemical characteristics such as the optimum temperature, pH, stable pH, and salt concentration were examined for each enzyme. Types of product inhibition and <jats:italic>K<jats:sub>i<\/jats:sub><\/jats:italic> values with <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> and <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub> values of the substrates and coenzymes were determined. According to the obtained <jats:italic>K<jats:sub>i<\/jats:sub><\/jats:italic> and IC<jats:sub>50<\/jats:sub> values, furosemide, digoxin, and dopamine showed inhibitory effect on the enzyme activities at low millimolar concentrations in vitro conditions. Dopamine inhibited the activity of these enzymes as competitive, whereas furosemide and digoxin inhibited the activity of the enzyme as noncompetitive.<\/jats:p>","DOI":"10.1002\/jbt.21793","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T14:25:20Z","timestamp":1453991120000},"page":"295-301","source":"Crossref","is-referenced-by-count":12,"title":["Purification and Characterization of Glucose 6\u2010Phosphate Dehydrogenase, 6\u2010Phosphogluconate Dehydrogenase, and Glutathione Reductase from Rat Heart and Inhibition Effects of Furosemide, Digoxin, and Dopamine on the Enzymes Activities"],"prefix":"10.1002","volume":"30","author":[{"given":"Sevki","family":"Adem","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science Cankiri Karatekin University  18000 Cankiri Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet","family":"Ciftci","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Arts and Science Faculty Bingol University  12000 Bingol Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-04-077412"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.05.006"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(03)00043-X"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/106002809502901213"},{"issue":"9","key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.17221\/5712-VETMED","article-title":"Purification and characterization of glucose 6\u2010phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes","volume":"49","author":"Ciftci M","year":"2004","journal-title":"Vet Med\u2010UZPI"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-012-9726-x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2014.04.001"},{"issue":"1","key":"e_1_2_5_9_1","first-page":"142","article-title":"Purification and characterization of glutathione reductase from camel (Camelus dromedaries) erythrocytes","volume":"48","author":"Al\u2010Senaidy AM.","year":"2010","journal-title":"Eur J Sci Res"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21592"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21606"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.3.489"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00721.2012"},{"issue":"2","key":"e_1_2_5_14_1","first-page":"64","article-title":"Purification and characterization of the tissue\u2010bound semicarbazide\u2010 sensitive amine oxidase (SSAO) from rat lung","volume":"30","author":"Yabano\u01e7lu S","year":"2005","journal-title":"Fabad J Pharm Sci"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2011.08.031"},{"key":"e_1_2_5_16_1","volume-title":"Red cell metabolism manual of biochemical methods","author":"Beutler E.","year":"1971"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(85)13062-4"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_5_19_1","doi-asserted-by":"crossref","unstructured":"Laemmli UK. 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